A Desulfurization and Denitrification Paracoccus and Its Application
A technology for denitrifying paracocci and colonies, which is applied in the direction of bacteria, separation methods, biological water/sewage treatment, etc., and can solve the problems of slow growth, slow desulfurization rate, and difficulty in obtaining
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Embodiment 1
[0028] Embodiment 1: strain screening
[0029] Take an appropriate amount of sludge from the aerobic aeration tank of Chengdu No. 2 Sewage Treatment Plant, and add it into a reactor containing 5L of sodium thiosulfate medium at an inoculum size of 20%. Under the conditions of enrichment culture for 7 days, sodium sulfide medium was used for plate separation. After 3 to 4 days, colonies were formed. Pick a single colony and insert it into the plate for culture. Repeat the streak separation for more than 4 times to obtain pure bacteria.
Embodiment 2
[0030] Embodiment 2: bacterial strain identification
[0031] The strain was inoculated in sodium thiosulfate medium according to the same inoculum size, and cultured in a shaker at different temperatures (20°C, 25°C, 30°C, 40°C) for 30 hours, and the growth temperature of the strain was determined to be 20°C~ 35°C, the optimum growth temperature is about 30°C; take the initial pH values of 5.0, 5.5, 6.0, 7.0, 8.0, 8.5, and 9.0 to culture for 30 hours at the optimum growth temperature, and determine that the growth pH of the bacteria is 5.5-9.0. Optimum growth pH is 7.0.
[0032] The isolated and purified bacterial strain was observed under a scanning electron microscope (SEM), and the results were as follows: figure 1 As shown; the bacterium is spherical, 0.2-0.8 μm long, 0.2-0.4 μm wide, without flagella; the bacterium is positive by Gram staining.
[0033] The entire genome of the pure strain was extracted using a bacterial whole genome rapid extraction kit, PCR amplifi...
Embodiment 3
[0035] Embodiment 3: desulfurization bottle test
[0036] 200ml sodium sulfide medium solution (800mg / L S 2- ) was sterilized under ultraviolet light for 30 minutes, put it into a sterilized 500ml Erlenmeyer flask under aseptic conditions, then add 50ml of denitrifying paracoccus bacteria liquid cultivated for 30h, stopper the bottle mouth with an aerobic stopper, and place it on a shaker Medium oscillation (30°C, 200r / min), the initial sulfide concentration was determined to be 582mg / L S 2- ; The sodium sulfide medium formula is: Na 2 S.9H 2 O 10.0g, KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, NH 4 Cl 0.2g, MgCl 2 0.2g, NaHCO 3 1.0g, 1ml of trace element solution, 1ml of vitamin solution, adjust the pH to neutral with 1mol / L HCl solution.
[0037] The same amount of sterile water was added to the control group, and three replicates were set up in each experimental group, and the sulfide concentration and elemental sulfur concentration were measured by sampling every 30 minu...
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