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A method for extracting oil from microalgae

A technology of microalgae oil and microalgae, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of limited effect of alcohol dissolution, achieve complete crushing, shorten reaction time, and simple process

Active Publication Date: 2020-08-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, a short-chain alcohol solvent is added after the microalgae are subjected to microwave treatment to destroy the cells. Although the short-chain alcohol solvent has a certain dissolution effect on the cell membrane phospholipid layer after the cell destruction, the effect of alcohol dissolution is limited, and the short-chain alcohol Mainly involved in base-catalyzed reactions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The microalgae is chlorella vulgaris, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.82g / L (dry weight basis). Take 5L of the microalgae liquid and carry out centrifugation, control the centrifugal rotation speed to 4000 rpm, and centrifuge for 3 minutes to obtain the microalgae mud. Add 0.03mol / L Na to the microalgae mud 2 CO 3 solution 45ml and 10% sodium hydroxide solution 45ml. After mixing, place the above system in a microwave generator, set the power of the microwave generator to 5kW, microwave frequency to 2450MHz, heating temperature to 50°C, and heat for 20min to carry out cell disruption and oil saponification. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1mol / L Na to the liquid system 2 CO 3 Solution 90ml, make the fatty acid salt precipitate, filter and collect the solid; dissolve the...

Embodiment 2

[0030] The microalgae is botrytis, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.78g / L (dry weight basis). Take 5L of microalgae liquid for centrifugation, control the centrifugation speed to 4000rpm, and centrifuge for 5 minutes to obtain microalgae mud, dry and grind the microalgae mud to obtain 8.9g of microalgae powder. Add 0.02mol / L NaNO to the microalgae powder 3 Solution 58ml and 30% sodium hydroxide solution 29ml. After mixing, place the above system in a microwave generator, set the power of the microwave generator to 10kW, microwave frequency to 915MHz, heating temperature to 55°C, and heat for 30min to carry out cell disruption and oil saponification. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1.5mol / L NaNO to the liquid system 3 Solution 45ml, make fatty acid salt precipitate, filter and col...

Embodiment 3

[0032] The microalgae is botrytis, which was cultured in a 10L photobioreactor for 10 days. At the end of the culture, the concentration of algae cells was analyzed to be 1.92g / L (dry weight basis). Take 5L of microalgae liquid for centrifugation, control the centrifugation speed to 4000rpm, and centrifuge for 5min to obtain microalgae mud, dry and grind the microalgae mud to obtain 9.6g of microalgae powder. Add 0.03mol / L of KH to the microalgae powder 2 PO 4solution 40ml and 10% potassium hydroxide solution 40ml. After mixing, place the above system in a microwave generator, set the power of the microwave generator to 15kW, microwave frequency to 915MHz, heating temperature to 65°C, and heat for 40min to carry out cell disruption and oil saponification. Then use a membrane with a pore size of 10 -1 μm microfiltration membrane to remove algae cells and debris. Continue to add 1.5mol / L of KH to the liquid system 2 PO 4 Solution 48ml, make fatty acid salt precipitate, fil...

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Abstract

The invention discloses a method of extracting microalgae fat, which includes the following steps: (1) adding certain amounts of an inorganic salt solution and an alkaline solution to collected microalgae, uniformly mixing the components, and performing radiation heating in a microwave generator, so that cell walls are dissolved and ruptured by means of cooperative effects of alkali, salt and microwave; (2) continuously performing a saponification reaction to alkali and fatty acid esters released from the cells to generate fatty acid salts; (3) filtering the liquid to remove microalgae cell debris; (4) continuously adding the inorganic salt solution to the reaction system to separate the fatty acid salt solution out, and filtering the liquid to collect a solid; and (5) adding inorganic acid to the fatty acid salts to acidify the fatty acid salts to obtain free fatty acid, and collecting the fatty acid product. In the invention, the microalgae fat is extracted through a water-phase method, so that the method is simple in operation process, is high in yield of the microalgae fat and is environment-friendly.

Description

technical field [0001] The invention relates to a method for extracting oil from microalgae, in particular to a method for extracting oil from microalgae using an aqueous phase method. Background technique [0002] Microalgae are a class of lower plants that grow in water and are diverse and widely distributed. This type of organism has an efficient photosynthetic reaction system, which can 2 Light energy is converted into chemical energy and stored in the body cells in the form of organic matter such as oil or starch. With the pressure of shortage of resources in human society and the increasingly serious environmental problems, the development of biodiesel and some fossil energy substitutes using microalgae has become a research hotspot. [0003] The use of microalgae for biodiesel production is a complex system engineering, covering multiple technical links, including screening and cultivation of microalgae species, large-scale cultivation of microalgae and induction of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 李晓姝高大成师文静廖莎孙启梅王鹏翔
Owner CHINA PETROLEUM & CHEM CORP
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