Preparation method of EMPLGA/Gel-HA hydrogel-microsphere bionic composite support

A composite scaffold and hydrogel technology, applied in the field of preparation of biomimetic composite scaffolds, can solve the problems of low mechanical strength, difficult disinfection, and poor shape retention ability of hydrogel porous materials, achieve superior biological properties, and expand the scope of regulation. , the effect of high mechanical strength

Inactive Publication Date: 2017-05-10
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, porous hydrogel materials have disadvantages such as low mechanical strength, poor shape retention ability, and difficulty in sterilization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Add 78.0g of dried D,L-lactide and 22.0g of glycolide into the dried ampoule, and then add 0.015g of dehydrated stannous octoate (Sn(Oct) 2 ); After vacuum drying for 12 h, seal the tube with an alcohol blowtorch under the condition of 4.0 Pa. Put the sealed ampoule into a thermostat, react at 140°C for 24 hours, break the ampoule, and take out the crude product PLGA. Product M w =1.76×10 5 Da, M w / M n =1.21.

[0034] The crude product was dissolved in trichloromethane to prepare a 2.0 wt% solution, dropped into 3 times the volume of ethanol and precipitated 3 times, and dried in vacuum. The purified PLGA was mixed with 6.0 g of maleic anhydride and 0.20 g of dibenzoyl peroxide, placed in a three-necked flask, and reacted for 24 hours at a pressure of 0.084 MPa and 100° C. to obtain a crude MPLGA polymer. The grafted crude polymer was placed in a Soxhelt extractor, and chloroform was used as a solvent to extract for 8 hours. The extracted residue was vacuum dried and dis...

Embodiment 2

[0041] Add 76.0g of dried D,L-lactide and 24.0g of glycolide into the dried ampoule, and add 0.022g of dehydrated stannous octoate (Sn(Oct) 2 ); After vacuum drying for 12 h, seal the tube with an alcohol blowtorch under the condition of 5.0Pa. Put the sealed ampoule into a thermostat and react for 36 h at 120°C. Break the ampoule to obtain the crude product PLGA. Product M w =1.45×10 5 Da, M w / M n =1.31.

[0042] The crude product was dissolved in chloroform to prepare a 3.0wt% solution, dropped into 4 times the volume of ethanol and precipitated 4 times, and dried in vacuum. The purified PLGA was mixed with 6.5g maleic anhydride and 0.25g dibenzoyl peroxide, placed in a three-necked flask, and reacted for 24h under the conditions of a pressure of 0.065MPa and 100°C to obtain a crude MPLGA polymer. Place the grafted crude polymer in a Soxhelt extractor and extract with chloroform as a solvent for 8 hours. The residue after extraction is vacuum dried and dissolved in chloroform...

Embodiment 3

[0049] Add 73.0g of dried D,L-lactide and 27.0g of glycolide into the dried ampoule, and then add 0.025g of dehydrated stannous octoate (Sn(Oct) 2 ); After vacuum drying for 12 h, seal the tube with an alcohol blowtorch under the condition of 6.0 Pa. Put the sealed ampoule in a thermostat, react at 130°C for 36 h, break the ampoule, and take out the crude product PLGA. Product M w =1.85×10 5 Da, M w / M n =1.23.

[0050] The crude product was dissolved in chloroform to prepare a 4.0wt% solution, dropped into 5 times the volume of ethanol and precipitated 5 times, and dried in vacuum. The purified PLGA was mixed with 6.8 g maleic anhydride and 0.18 g dibenzoyl peroxide, placed in a three-necked flask, and reacted for 24 hours at a pressure of 0.076 MPa and 100° C. to obtain a crude MPLGA polymer. The grafted crude polymer was placed in a Soxhelt extractor, and chloroform was used as a solvent to extract for 8 hours. The residue was vacuum dried and dissolved in chloroform to prepa...

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Abstract

The invention provides a preparation method of an EMPLGA/Gel-HA hydrogel-microsphere bionic composite support. The preparation method comprises the following steps: preparing high-molecular weight poly lactide-glycolide acid (PLGA) through melt polymerization under high vacuum, reducing the pressure and grafting maleic anhydride at a temperature of 100 DEG C to obtain maleinized poly lactide-glycolide acid (MPLGA); conducting reaction on quadrol and MPLGA for 30-60 minutes in a chloroform solution at a room temperature, precipitating, vacuum-drying to obtain a quadrol-modified MPLGA polymer and preparing a porous MPLGA hard support by using a pore-forming method; adding a prepared mixed aqueous solution of gelatin/hyaluronic acid into liquid paraffin containing span-80, performing glutaraldehyde cross-linking and solidifying into a sphere; respectively adding gelatin and hyaluronic acid into a glacial acetic acid solution for dissolution, uniformly mixing, and adding a Gel/HA microsphere into a mixed solution to obtain a composite system; soaking the EMPLGA hard support material in the composite system, vacuum-pumping for 20-30 minutes, taking out, freezing, drying, soaking with an ethanol solution containing carbodiimide and ethanol, and vacuum-drying to obtain the EMPLGA/Gel-HA hydrogel-microsphere bionic composite support. The bionic composite support prepared by the preparation method provided by the invention has a natural tissue environment and is good in mechanical strength.

Description

Technical field [0001] The invention relates to the technical field of production of a degradable soft / hard bionic composite cartilage tissue scaffold material, in particular to a method for preparing a bionic composite scaffold with a natural tissue-like environment and good mechanical strength, and is expected to be applied to cartilage tissue engineering The field of stent materials. Background technique [0002] Cartilage defect caused by mechanical damage or arthritis is a long-term medical problem that is difficult to overcome. Adult cartilage repair ability is limited, cartilage defect diameter less than 3mm can be repaired spontaneously, but when the diameter is greater than 3mm, the defect site cannot be repaired by itself. Tissue engineering technology provides a new method for the repair of bone defects. It has the advantages of little damage to the donor, no antigenicity, can be made into three-dimensional, accurate anatomical structure, etc., so it has broad applica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/56A61L27/52A61L27/18A61L27/22A61L27/20C08G63/08
CPCA61L27/58A61L27/18A61L27/20A61L27/222A61L27/52A61L27/56A61L2430/06C08G63/08C08L89/00C08L67/04C08L5/08
Inventor 周智华张巧刘文娟潘一峰周虎刘清泉王卫
Owner HUNAN UNIV OF SCI & TECH
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