Ulva pertusa polysaccharide separation product and separation and purification method thereof

A technique for separating and purifying Ulva pore polysaccharides, which is applied in the field of polysaccharide extraction, can solve the problems of slow onset of blood lipid-lowering effect and large dosage of medication, and achieve the effect of enhancing blood lipid-lowering effect and reducing the burden of medication for patients

Inactive Publication Date: 2017-05-31
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with the blood lipid-lowering drugs commonly used in clinical practice, Ulva polysaccharides have a slow onset of blood lipid-lowering effect and a large dosage

Method used

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  • Ulva pertusa polysaccharide separation product and separation and purification method thereof
  • Ulva pertusa polysaccharide separation product and separation and purification method thereof
  • Ulva pertusa polysaccharide separation product and separation and purification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Extraction method of Ulva polysaccharide

[0031] (1) Pretreatment: Wash the Ulva algae body with tap water to remove silt, weeds and other attachments; then dry it naturally, pass through a 60-mesh sieve after crushing, and obtain the Ulva algae body powder, which is packaged for later use;

[0032] (2) Degreasing treatment: Weigh the Ulva pore algae body powder in step (1), reflux three times with 80% ethanol, centrifuge, and dry under reduced pressure at 40° C. to obtain degreasing Ulva pore algae body;

[0033] (3) Hot water extraction: extract the degreased Ulva pore algae bodies obtained in step (2) 3 times under the condition of a water bath at 125° C., extract 2 hours each time, filter, and combine the filtrates three times, then carry out rotary evaporation and concentration of the filtrates, and use 75% Precipitate with ethanol for 24h, filter out the precipitate;

[0034] (4) Drying: the precipitate filtered out in step (3) was dehydrated twice with absolute...

Embodiment 2

[0040] Separation and purification method of Ulva pore polysaccharide separation product

[0041] (1) Ultrasonic dissolution: Accurately weigh 10 g of the crude Ulva polysaccharide (prepared in Example 1) and place it in a 1000 ml beaker, add 1000 ml of distilled water, ultrasonically treat for 1 hour with 400W power, and stir intermittently to dissolve it;

[0042] (2) Centrifugal treatment: centrifuge the polysaccharide solution obtained in step (1) in a high-speed centrifuge at 8000r / min for 8min, and collect the supernatant;

[0043] (3) Ion-exchange column chromatography: use DEAE-Sepharose Fast Flow ion-exchange packing to pack into a 5cm×20cm column bed, equilibrate with 10 times of distilled water, and then pass 1L of the supernatant obtained in step (2) through DEAE-Sepharose FastFlow Ion exchange column, eluted with distilled water, 0.5mol / L sodium chloride solution, and 1mol / L sodium chloride solution in sequence, the elution flow rate is 10ml / min, collected in sect...

Embodiment 3

[0049] Scavenging effect of isolated products F1, F2 and F3 on superoxide anion radicals

[0050] The removal experiment reaction system contains polysaccharide samples with different concentrations and 435mol L -1 Reduced coenzyme I (NADH) 1mL, 150mol L -1 Nitro blue tetrazolium (NBT) 1mL, 72mol L -1 Phenazine methyl sulfate (PMS) solution 1mL, each of the above solutions is Tris-HCl buffer solution (16mmol L -1 , pH 8.0) preparation, after standing at room temperature for 5min, the absorbance was measured at a wavelength of 560nm. The blank group did not add sample and NADH solution, and the control group did not add sample solution. Clearance calculation formula:

[0051] Clearance (%) = (A 对照 -A 样品 ) / (A 对照 -A 空白 )100%

[0052] The clearance results are shown in Table 3.

[0053] Table 3 Scavenging effect of isolated products F1, F2 and F3 on superoxide anion free radicals

[0054]

[0055] The experimental results show that the F1, F2 and F3 sample solutions ...

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Abstract

The invention relates to the technical field of polysaccharide extraction and particularly relates to an ulva pertusa polysaccharide separation product which contains at least one of a separation product F1, a separation product F2 and a separation product F3. The separation product F1 is prepared from rhamnose, xylose and glucose according to a proportion of molar ratio of 1 : (0.29 to 0.35) : (0.13 to 0.15); the separation product F2 is prepared from rhamnose, xylose and glucose according to a proportion of molar ratio of 1 : (0.38 to 0.46) : (0.26 to 0.28); the separation product F3 is prepared from rhamnose and xylose according to a proportion of molar ratio of 1 : (0.36 to 0.44). The invention further relates to a separation and purification method of the separation product. The separation and purification method comprises the following steps of extracting ulva pertusa polysaccharide through a water extraction and alcohol precipitation method, performing ultrasonic dissolving treatment, performing centrifugal treatment, performing ion-exchange column chromatography treatment, performing dialysis treatment and freezing and drying to obtain the separation product F1, the separation product F2 and the separation product F3. The three separation products disclosed by the invention have high-efficiency antioxidant activity and establish base for researches on enhancing a blood fat reducing effect, reducing drug use burden of a patient and the like.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to an Ulva pore polysaccharide separation product and a separation and purification method thereof. Background technique [0002] Ulva pertusa (Ulva pertusa) belongs to Chlorophyta (Chlorophyta) Ulvaceae (Ulvaceae) Ulva genus (Ulva), is a kind of edible large green algae, which is widely distributed in the western part of the North Pacific Ocean, Korea, Japan, The coast of some sea areas of China and CIS Asia. In my country, it is mainly distributed in the coastal areas of Liaoning, Hebei, Shandong, Jiangsu and other provinces. Its larvae are green, and the adult color is dark green. [0003] Ulva pore polysaccharides are intercellular products and exist in the form of heteropolysaccharides in algae. Modern pharmacological studies have proved that Ulva polysaccharides have many physiological activities such as anti-oxidation, blood lipid regulation, anti-tumor an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 綦慧敏李伟达刘顺梅常新涛蒋南芳
Owner WEIFANG MEDICAL UNIV
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