Preparation method for guided tissue regeneration membrane for periodontitis repair

A technology for guiding tissue regeneration and periodontitis, applied in the field of preparation of guiding tissue regeneration membrane, can solve the problems of restricting the use of regeneration membrane, unfavorable bone repair, matching the degradation speed of bone tissue repair speed, etc. Responsiveness, enhanced adhesion and proliferation, improved biocompatibility

Inactive Publication Date: 2017-06-06
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Among polylactic acid polymer materials, although PLA has high strength, it degrades too slowly (the larger the molecular weight, the slower the degradation rate), it is easy to cause delayed tissue reactions (such as swelling at the implantation site, aseptic sinus formation, etc.) etc.), it is not conducive to bone repair, and its processing plasticity is also poor, which limits its use as a guided regeneration membrane
The modified PLGA film has strong plasticity and better biocompatibility, but the degradation rate is faster
The degradation speed of the two cannot match the speed of bone tissue repair, which greatly interferes with bone repair and is prone to adverse reactions

Method used

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  • Preparation method for guided tissue regeneration membrane for periodontitis repair
  • Preparation method for guided tissue regeneration membrane for periodontitis repair
  • Preparation method for guided tissue regeneration membrane for periodontitis repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Composite membrane preparation

[0027] (1) Add 4.17g serine, 1250g lactic acid and 1g catalyst stannous isooctanoate in the reaction vessel, react at 150°C under vacuum and reduced pressure until no water is distilled out, and the prepared molecular weight is 1955, and the intrinsic viscosity is 0.138 Low molecular weight lactic acid-serine polymer (PLAS), the synthetic PLAS is 4000~500cm -1 Infrared spectroscopy scans in the wavenumber range. In the infrared spectrum, 3340cm -1 The sharp absorption peak at represents the stretching vibration of the amino group in the polymer; 2943cm -1 The absorption peak at indicates that there is a saturated C-H stretching vibration in the polymer, and at 1361cm -1 There is an absorption peak at , indicating the presence of methyl-CH in the polymer 3 ;1746cm -1 The absorption peak at represents the existence of functional group C=O; 1266cm -1 The absorption peaks indicated the presence of C-O structure; these abs...

Embodiment 2

[0030] Embodiment 2: the biological barrier effect test of composite film

[0031] Use a punching machine to cut each group of membrane materials into small discs with a diameter of 6mm, all are fine 60 Co irradiated for 24 hours to sterilize, and irradiated with ultraviolet light for 30 minutes before use. Put the material into a 96-well culture plate 1 day before the experiment, pre-wet it with the culture medium, and set it aside.

[0032] Take the human gingival fibroblasts cultured until the cell density reaches about 80% of the culture bottle, digest with trypsin, count, and adjust the concentration of the cell suspension to 5×l0 3 / ml. Take 100 μL and inoculate it on the pre-wetted membrane material in the 96-well plate of each group, mark the side of the membrane material inoculated with cells as the front side, and the other side as the reverse side. 2 1. Cultivate under saturated humidity conditions, add 100 μL of culture solution to each well after 3 hours, conti...

Embodiment 3

[0035] Embodiment 3: In vitro degradation test of composite membrane

[0036] Through the in vitro degradation experiment of the composite film, its biodegradation behavior was studied, and whether it could meet the requirement that the oral barrier film needs to exist in the body for 4-6 months.

[0037] The experimental steps of the degradation test are as follows: ① prepare phosphate buffered saline; ② vacuum desiccate the sample to constant weight. The sample is cut into 2*5mm strips, divided into 20 parts, each with a mass of about 0.3000g, and recorded W 0 ;③ Add 15ml of buffer solution to 20 15ml screw-top bottles and number them, and put the samples in order; , 8, and 16 weeks to take out three samples; ⑤ vacuum-dry the filter paper to constant weight and record Wc, each sample is filtered by quantitative filter paper; ⑥ measure and record the mass Wd of the clean and dry centrifuge tube; ⑦ add the filtrate to the centrifuge tube After centrifuging for 10 minutes, ca...

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Abstract

The invention relates to a preparation method for a guided tissue regeneration membrane for periodontitis repair, wherein the preparation method comprises the following steps: (1) adding serine, lactic acid and a catalyst stannous isocaprylate to a reaction vessel, under a vacuum reduced pressure condition, carrying out a reaction at the temperature of 150 DEG C until no water is steamed out, to prepare a low-molecular-weight PLAS; (2) adding lactide, glycolide and serine in the reaction vessel, under the action of an initiator stannous isocaprylate, carrying out a reaction for 3 hours in vacuum oil bath at the temperature of 150 DEG C, to obtain a high-molecular-weight PLGAS; and (3) mixing evenly the PLAS and the PLGAS, crushing, adding a solvent, stirring until the components are completely dissolved, to obtain a solution A, and brushing to form a membrane by a membrane coating device. The guided tissue regeneration membrane prepared by the method has the advantages of good biocompatibility, basically consistent degradation speed and bone tissue repair, little interference on bone repair, difficult appearing of delayed tissue response, and excellent physical properties, and can be applied in skeleton pads for periodontitis repair.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a guided tissue regeneration film used for periodontitis repair. Background technique [0002] Periodontitis is an oral disease caused by related microorganisms. Its main clinical manifestations include swelling and bleeding of the gums, formation of periodontal pockets, resorption of root bone, and loose teeth. In addition, some systemic diseases such as cardiovascular disease and diabetes are also closely related to periodontitis. Periodontitis often leads to the loss of surrounding bone tissue and loose teeth. Traditional periodontal treatment often cannot effectively improve the food impaction and aesthetic problems caused by periodontal bone defect. Therefore, how to repair the defective periodontal bone tissue and reduce the Tooth loss has become a hot spot in periodontal disease treatment. The guided bone regeneration (GBR) technology that has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/56A61L27/26
CPCA61L27/50A61L27/26A61L27/56A61L2430/02C08L77/12
Inventor 赵海兵居保国金志敏
Owner ZHEJIANG UNIV OF TECH
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