Recombinant transaminase as well as preparation method and application of recombinant transaminase

A transaminase and amino acid technology, applied in the field of bioengineering, can solve problems such as low product yield and chiral purity, harsh reaction conditions, and long synthetic routes, and achieve excellent stereoselectivity, high reaction conversion rate, and high product yield. The effect of mild reaction
CN106801043AActive Publication Date: 2017-06-06JIANGSU ALPHA PHARM CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU ALPHA PHARM CO LTD
Publication Date
2017-06-06

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Abstract

The invention discloses recombinant transaminase as well as a preparation method and application of the recombinant transaminase. An amino acid sequence of a high-activity recombinant transaminase mutant is as shown in SEQ ID NO.2 and a coding gene is as shown in SEQ ID NO.1. The method for preparing the recombinant transaminase compries the steps of fermenting and cultivating a gene engineering bacterium containing the coding gene, and collecting and preparing the recombinant transaminase. The recombinant transaminase is applied to asymmetric synthesis of a chiral amine compound, and particularly is applied to synthesis of a sitagliptin midbody (R)-3-amino-4-(2,4,5-trifluoromethyl phenyl)-methyl butyrate. The related transaminase has excellent stereoselectivity, regioselectivity and catalytic activity, the catalytic reaction is mild, the reaction conversion rate and the yield of a product are high, and the recombinant transaminase has relatively good application prospect.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a recombinant transaminase and its preparation method and application. Background technique

[0002] Diabetes is a systemic chronic progressive disease caused by the body's own metabolic disorder, which is caused by insufficient insulin secretion or insulin action disorder. Diabetes is usually accompanied by complications such as coronary heart disease, cerebrovascular disease, nephropathy, and necrosis of lower extremities. It is a complex and frequently-occurring disease. At present, the number of adults with diabetes in the world is 422 million, and it is estimated that by 2025, more than 700 million people worldwide will suffer from diabetes.

[0003] Diabetes is mainly divided into type Ⅰ and type Ⅱ. Type Ⅱ diabetes is non-insulin-dependent diabetes, which is caused by insulin resistance or impaired function of pancreatic β cells. Its patients account for...

Claims

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