Preparation method of olive leaf extract

Abstract

The invention discloses a preparation method of an olive leaf extract. The preparation method comprises the following steps: a, selecting olive leaves; b, putting the selected olive leaves into saline water, and soaking for 1-2 hours; c, drying; d, after drying, grinding into fine powder; e, adding 3-8 times of water into the raw material, and then adding cellulase; f, after enzymatic hydrolysis, filtering to obtain an enzymatic hydrolysate; g, directly enabling the enzymatic hydrolysate to pass through a neutral aluminum oxide column with the diameter-height ratio of 1:4 to 1:13, eluting for removing impurities by using water and an ethanol solution with the concentration of 90% respectively, eluting by using a mixture of glacial acetic acid with the concentration of 0.2-0.5% and an ethanol solution with the concentration of 45%, and collecting an eluate, wherein the volume of water is 2-3 times of the column volume, the volume of the ethanol solution with the concentration of 90% is 0.8-1.5 times of the column volume, and the volume of the mixture is 7-9 times of the column volume; h, volatilizing the glacial acetic acid and the ethanol in the eluate, and performing microwave vacuum drying to obtain the olive leaf extract. By the preparation method, active components can be dissolved out more effectively, and energy consumption in the extraction process is greatly reduced and thus the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of natural plant extraction, and in particular relates to a preparation method of olive leaf extract. Background technique [0002] Olive leaves mainly contain secoiridoids and their glycosides, flavonoids and their glycosides, biflavones and their glycosides, low-molecular tannins and other components, and secoiridoids are the main active ingredients. Oleuropein is the bitter element in olive leaves. It is a bimolecular structure of phenols and flavonoids with a molecular weight of 540.52 and a melting point of 87-89°C. It is easy to absorb moisture and is easily soluble in ethanol, acetone, pyridine, etc. , butanol, ethyl acetate, etc., almost insoluble in ether, petroleum ether, chloroform, etc. According to reports, oleuropein has important health functions such as natural anti-oxidation, anti-tumor, anti-aging, enhancing cardiovascular function, treating chronic prostatitis, enhancing immunity, mediati...

AI Technical Summary

Problems solved by technology

However, organic solvent extraction has the disadvantages of high impurity content, large solvent consumption, high cost, and large pollution.

Supercritical carbon dioxide extraction has the advantages of high extraction content and no pollution, but it has a large investment and high cost

Although ultrasonic and microwave extraction have the advantages of energy saving and high efficiency, it is difficult to achieve large-scale application in production