Preparation method of olive leaf extract
A technology of olive leaf extract and olive leaf, applied in the direction of pharmaceutical formula, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of large pollution, large solvent consumption, difficulty in large-scale application, etc., and achieve reduction Energy consumption and cost reduction effects
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[0017] Embodiment: the present invention comprises the following steps,
[0018] a, select olive leaves, select fresh, undamaged olive leaves;
[0019] b. Soak the selected olive leaves in salt water for 1-2 hours;
[0020] c, drying, the olive leaves after soaking are dried;
[0021] d, after drying, pulverize and grind into fine powder;
[0022] e. Add 3-8 times of water to the raw material, then add cellulase: alkaline protease compound enzyme, the amount of compound enzyme is 0.8%-1.5% of the weight of the raw material, and keep it at 30-50 degrees Celsius for enzymatic hydrolysis of 0.5-1.5 h;
[0023] f. Filtrating after enzymolysis to obtain an enzymolysis solution;
[0024] g. The enzymatic solution directly passes through the neutral alumina column, and the resin column diameter-to-height ratio is 1:4-1:13, and is eluted with 2-3 times column volume water and 0.8-1.5 times column volume 90% ethanol solution respectively. Miscellaneous, elute with 7-9 times column...
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