Kineococcus cavernae sp. and application thereof

A technology of dynamic coccus and cave, applied in the field of microorganisms, can solve the problem of no public report of dynamic coccus cave, and achieve the effects of expanding the richness, rounding the smoke, and enriching the aroma of the smoke

Inactive Publication Date: 2017-09-15
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Zoonococcus burrowing bacteria provided by the present invention was isolated for the first time, and was identified as a new species of microorganisms. At present, there is no public report about the Zoonidiococcus burrowing bacteria and related applications.

Method used

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  • Kineococcus cavernae sp. and application thereof
  • Kineococcus cavernae sp. and application thereof
  • Kineococcus cavernae sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] ——Isolation of Kinetococcus burrowing bacteria SYSUZL-1

[0022] Soil samples were collected from karst caves adjacent to the Yinyanzhuang tobacco field in Shilin County, Yunnan Province, sealed in plastic bags, and stored at 4°C until use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake at 30°C and 180rpm for 30min. Dilute to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Concentration, respectively take 0.2mL spread on LB plate culture medium, set 3 replicates for each. After culturing at 30°C for 48 hours, different single colonies were picked from the plates of each soil sample, purified on the corresponding solid medium, added glycerol at 20% of the volume of the bacterial solution, and stored in a -80°C refrigerator for later use.

[0023] The isolated microorganisms were respectively inoculated into LB liquid medium, shaken and cultivated at 30°C and 160 rpm on a shaker, and the bacteria were cultured overnight until the concentr...

Embodiment 2

[0025] ——Identification of Kinetococcus burrowing bacteria SYSUZL-1

[0026] The isolated and purified SYSUZL-1 was identified by morphology, physiology, biochemistry and molecular biology. The classification of SYSUZL-1 was named Kineococcus cavernae sp.nov.

[0027] The micrographs and electron micrographs of SYSUZL-1 on R2A medium are attached figure 1 .

[0028] SYSUZL-1 grows well on most media, and the hyphae in the substrate are reddish brown to brown. Gelatin liquefaction, milk coagulation and peptonization are positive, starch hydrolysis and nitric acid reduction are negative, hydrogen sulfide is not produced, and melanin is formed. Can utilize glucose, galactose, fructose xylose, raffinose, rhamnose, melibiose, sweet alcohol, glycerin, mannitol, sorbitol, sodium acetate. The cell wall contains L-diaminopimelic acid and glycine. (Table 1-3)

[0029] Table 1 Culture characteristics of SYSUZL-1

[0030]

[0031] Table 2 Physiological and biochemical characteri...

Embodiment 3

[0040] ——Application of Kinetococcus burrowing bacteria SYSUZL-1 in the preparation of tobacco flavor by fermentation

[0041] The application of the above-screened Kinetococcus burrowing bacteria SYSUZL-1 in the fermentative production of tobacco spices, the specific steps are as follows:

[0042] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000ml, pH7.2; culture temperature is 28°C, culture time is 7d.

[0043] (2) Seed culture: pick part of the mycelium from the slope and put it into the seed liquid, and shake the flask for 36 hours. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃.

[0044] (3) Fermentation culture: Inoculate 10% of the inoculum into the fermentation medium, and culture in shake flasks for 6 days. Medium: soybean powder 10g; g...

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Abstract

The invention discloses a kineococcus cavernae sp. and an application thereof. The strain is named kineococcus cavernae sp. SYSUZL-1, and is preserved in the China Center For Type Culture Collection (CCTCC) on December 9, 2016, and the preservation number is M2016738. A microbiological characteristic analysis and a 16SrRNA gene sequence analysis indicate that the strain is a novel bacterium. The strain can produce benzaldehyde and volatile compounds including geosmin, elemene, elemol and Alpha-eudesmol, and after fermentation, a tobacco flavor can be prepared. The obtained flavor has unique fragrance, can enhance a cigarette style, and can enrich cigarette fragrance, so that the fragrance is fuller, moreover, the flavor has antibacterial and antifungal activity, and the effect is stable and persistent. In addition, the strain also has a tremendous potential in the development of novel, highly effective, nontoxic and natural medicines.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a zoonococcus. At the same time, the present invention also relates to the application of the Zoonococcus burrowing bacteria. Background technique [0002] There are many types of microorganisms. For a long time, people have used pure culture technology to investigate, research, develop and utilize microorganisms in the environment. With the development of science and technology, the importance of microbial resources in scientific research and biological industry has become increasingly prominent. Many countries in the world are collecting and preserving various microbial resources, and their continuous enrichment will lay a solid material foundation for the development of my country's biotechnology. [0003] Flavoring is a key link in the cigarette process, and it is one of the effective means to effectively improve the smoking quality of cigarettes and form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B3/12C12R1/01
CPCA24B3/12C12N1/205C12R2001/01
Inventor 段焰青陈兴李源栋汪显国李文均雷声曾熠程
Owner CHINA TOBACCO YUNNAN IND
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