Transformed fungus having enhanced ergothioneine productivity and method for producing ergothioneine

Abstract

The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) An enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5'-phosphate as a coenzyme.

Description

[0001] Cross-reference of related applications

[0002] This application claims the priority of Japanese Patent Application No. 2015-17328 filed on January 30, 2015 and Japanese Patent Application No. 2015-157444 filed on August 7, 2015 , and is incorporated herein by reference in its entirety. technical field

[0003] The present invention relates to a fungus with recognized ergothioneine production capacity and a method for preparing ergothioneine using the fungus. Background technique

[0004] The following formula [II]:

[0005]

[0006] The indicated ergothioneine is a sulfur-containing amino acid and is a biological substance that has been confirmed to exist in organs such as the liver or blood of animals including humans.

[0007] Ergothioneine is known to have antioxidant activity, and is reported to have, for example, a hydroxyl radical trapping action, a hydroxyl radical generation inhibitory action from iron or copper-dependent hydrogen peroxide, a copper-dep...

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