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Artificial blood vessel based on porcine small intestinal submucosa and preparation method of artificial blood vessel

A technology of artificial blood vessels and submucosa, applied in coatings, medical science, prostheses, etc., can solve the problems of low long-term patency rate, slow blood flow, thrombosis, etc., and achieve excellent antithrombotic performance, good effect, Resistant to the effect of blood washout

Inactive Publication Date: 2017-11-03
苏州期佰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the simple SIS material is implanted in the body, it is very easy to receive host cells, and the trauma reaction caused by the implantation operation often leads to excessive proliferation of fibroblasts, and finally forms a stricture with scar repair.
[0005] At present, large and medium-caliber vascular grafts have achieved good clinical results, and long-term patency is ideal. However, small-caliber (inner diameter less than 6 mm) artificial blood vessels are prone to thrombosis or endocrine disorders due to their slow blood flow and low blood pressure. Membrane dysplasia, resulting in low long-term patency after transplantation
Among them, the mismatch between the compliance of the host blood vessel and the artificial blood vessel will lead to the change of the hemodynamic state of the blood, which will cause thrombus formation and lead to the failure of the artificial blood vessel transplantation.

Method used

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  • Artificial blood vessel based on porcine small intestinal submucosa and preparation method of artificial blood vessel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 1.0g of freeze-dried SIS sample, grind it into powder with a grinder, transfer it to a test tube, add magnetron and 90mL of purified water, stir and mix evenly, add 500mg of trypsin, and stir the reaction at 50 degrees After 5 hours of enzymatic hydrolysis, boil to inactivate the enzyme, and obtain the degradation solution.

[0025] At room temperature, add polyurethane particles into tetrahydrofuran, and stir at a speed of 100 rpm for 2 hours; place the polyurethane solution in an environment with a vacuum of 0.3Mpa for defoaming; after standing for 3 hours, take out the polyurethane solution and squeeze it. The polyurethane solution produced is pretreated by a coagulation bath, and then the filamentary polyurethane is pulled out from the traction device, and then the filamentary polyurethane is wound on the rotating cylindrical mold; the cylindrical mold wound with the filamentary polyurethane Soak in absolute ethanol for 36 hours for coagulation bath qualitativ...

Embodiment 2

[0029] Weigh 1.0g of freeze-dried SIS sample, grind it into powder with a grinder, transfer it to a test tube, add magnetron and 95mL of purified water, stir and mix evenly, add 400mg of trypsin, and stir at 37 degrees to react After 24 hours, after the end of enzymatic hydrolysis, boil to inactivate the enzyme to obtain the degradation solution.

[0030]At room temperature, add polyurethane particles into tetrahydrofuran, and stir at a speed of 100 rpm for 2 hours; place the polyurethane solution in an environment with a vacuum of 0.3Mpa for defoaming; after standing for 3 hours, take out the polyurethane solution and squeeze it. The polyurethane solution produced is pretreated by a coagulation bath, and then the filamentary polyurethane is pulled out from the traction device, and then the filamentary polyurethane is wound on the rotating cylindrical mold; the cylindrical mold wound with the filamentary polyurethane Soak in absolute ethanol for 36 hours for coagulation bath q...

Embodiment 3

[0034] Weigh 1.0g of freeze-dried SIS sample, grind it into powder with a grinder, transfer it to a test tube, add magnetron and 90mL of purified water, stir and mix evenly, add 600mg of trypsin, and stir the reaction at 40 degrees After 12 hours of enzymatic hydrolysis, boil to inactivate the enzyme and obtain the degradation solution.

[0035] At room temperature, add polyurethane particles into tetrahydrofuran, and stir at a speed of 100 rpm for 2 hours; place the polyurethane solution in an environment with a vacuum of 0.3Mpa for defoaming; after standing for 3 hours, take out the polyurethane solution and squeeze it. The polyurethane solution produced is pretreated by a coagulation bath, and then the filamentary polyurethane is pulled out from the traction device, and then the filamentary polyurethane is wound on the rotating cylindrical mold; the cylindrical mold wound with the filamentary polyurethane Soak in absolute ethanol for 36 hours for coagulation bath qualitativ...

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Abstract

The invention discloses an artificial blood vessel based on porcine small intestinal submucosa as well as a preparation method and application of the artificial blood vessel. The preparation method comprises the following steps: adding the crushed porcine small intestinal submucosa into water, then adding trypsinase when the pH is 7.5-8.5 and performing enzyme inactivation after enzymatic hydrolysis to obtain degradation liquid; and coating the inner wall of the artificial blood vessel with the degradation liquid and drying to obtain the artificial blood vessel based on the porcine small intestinal submucosa. The artificial blood vessel as well as the preparation method and the application thereof disclosed by the invention has the benefits that the SIS degradation liquid is creatively used for modifying the biological artificial blood vessel, so that the biological artificial blood vessel with anti-thrombosis is constructed, and the problem of an existing small-diameter artificial blood vessel that thrombosis is easy to generate is effectively solved; and moreover, the preparation process is simple and reasonable, and the clinical application is facilitated.

Description

technical field [0001] The invention relates to a medical material, in particular to an artificial blood vessel based on the submucosa of pig small intestine and a preparation method thereof. Background technique [0002] When the blood vessels in a certain part of the human body cannot guarantee the normal blood supply to the human body due to reasons such as aging, arteriosclerosis, embolism or damage, surgical operations such as artificial blood vessel replacement, bypass or intervention are required for treatment. Ideal artificial blood vessels have good histocompatibility, antithrombotic properties, physical stability, anti-infection and ease of use, while excellent mechanical properties and good blood histocompatibility are the key to breakthroughs in artificial blood vessel technology . [0003] A large number of studies have shown that the epithelial cells of the small intestinal mucosa of normal animals show that the mucus layer contains a large amount of mucin, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/28A61L27/18A61L27/50A61L27/54
Inventor 尹华清方帆
Owner 苏州期佰生物技术有限公司
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