Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of broad-spectrum antibacterial amphiphilic biological carbon material and its preparation method and application

A technology of biophilic and carbon materials, which is applied in the fields of broad-spectrum antibacterial amphiphilic biocarbon materials and their preparation methods and uses, can solve the problems of complex and cumbersome surface modification processes, and achieve antibacterial lasting stability, reusability, and wide application. Field of application, effect of strong antibacterial effect

Active Publication Date: 2018-10-09
郑州欧姆康生物材料有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the surface of these hydrophilic polymers can induce the formation of a hydration film on the surface to prevent / repel the approach of proteins and the attachment of bacteria through strong hydrogen bonds or ionic interactions between the hydrophilic polymer and water, however, the hydrophilic polymer The antifouling performance of materials is highly dependent on surface hydration, highly dependent on the physical and chemical properties of the surface (material surface properties, roughness, graft density and thickness of hydrophilic polymers on the material surface, etc.), resulting in a complex and difficult surface modification process. redundant

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of broad-spectrum antibacterial amphiphilic biological carbon material and its preparation method and application
  • A kind of broad-spectrum antibacterial amphiphilic biological carbon material and its preparation method and application
  • A kind of broad-spectrum antibacterial amphiphilic biological carbon material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of amphiphilic bio-carbon material with yeast as raw material

[0041] Weigh 10g of dry beer yeast into a beaker, add 100mL of distilled water to shake and disperse, transfer the gray-white bacterial suspension in the upper layer to another beaker, centrifuge at 8000r / min for 5min, discard the waste liquid, and wash with 30ml of acetone for 5 minutes (shake for 10 min before each centrifugation), then disperse the substrate in 2% glutaraldehyde aqueous solution, pour it into a high-temperature and high-pressure reactor, and react for 10 h at 160 ° C. After the reaction, remove the dark brown insoluble film , Pour off the yellow-brown solution in the upper layer, disperse the precipitate in the lower layer with distilled water, wash it repeatedly with ultrasonic waves, and finally place it in an oven to dry.

Embodiment 2

[0042] Example 2 Preparation of amphiphilic bio-carbon material with Escherichia coli as raw material

[0043] Centrifuge the bacteria solution containing Escherichia coli, and then wash it 3 times with acetone (shaking for 15 minutes before each centrifugation), then disperse the substrate in 2% glutaraldehyde aqueous solution, pour it into a high-temperature and high-pressure reaction kettle, and React at 180°C for 8 hours. After the reaction, remove the dark brown insoluble film, pour off the upper layer of yellowish brown solution, disperse the lower layer of precipitate with distilled water, repeatedly ultrasonically wash, and finally place it in an oven to dry.

Embodiment 3

[0044] Embodiment 3 prepares quaternary ammonium salt antibacterial amphiphilic biological carbon material

[0045] (1) Amphiphilic biocarbon materials modified with allylamine (ACPs-AM)

[0046] Weigh 150 mg of dried amphiphilic bio-carbon material and disperse it in 25 mL of PB buffer (pH 5.5), add 75 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide in sequence Hydrochloride, N-hydroxysuccinimide, and allylamine hydrochloride were fully activated on a shaker for 12 hours. Centrifuge after the reaction is over, discard the supernatant, add distilled water and ultrasonically wash repeatedly several times, and air-dry at 30°C for 12 hours.

[0047] (2) Continuous grafting of poly-4-vinylpyridine (ACPs-AM-P4VP) on the surface of amphiphilic biocarbon materials

[0048] Dissolve 150mg of N,N-methylenebisacrylamide ultrasonically in 20mL of acetonitrile, and dissolve a small amount of initiator azobisisobutyronitrile in 5mL of acetonitrile, combine the two solutions, add 6mL ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a broad-spectrum antibacterial amphiphilic biological carbon material, and a preparation method and an application thereof. Through a covalent coupling method and a distillation-precipitation polymerization method, a broad-spectrum antibacterial agent of quaternary ammonium salt is uniformly fixed to the surface of an amphiphilic biological carbon material to obtain a quaternary-ammonium-salt-fixed amphiphilic biological carbon material, which is the broad-spectrum antibacterial amphiphilic biological carbon material. The broad-spectrum antibacterial amphiphilic biological carbon material has advantages of broad-spectrum antibacterial property, antibacterial persistence and stability, and reutilization, and has a preferable antibacterial effect on gram-positive bacterium and gram-negative bacterium. The invention further discloses the preparation method of the broad-spectrum antibacterial amphiphilic biological carbon material.

Description

technical field [0001] The invention relates to a broad-spectrum antibacterial amphiphilic biological carbon material and its preparation and application in antibacterial coating materials. Background technique [0002] Surface nonspecific adsorption / adhesion of proteins / bacteria is an important reason for surface biofouling. Biofouling is a serious hazard for applications in the fields of chips, textiles, food packaging and transportation. For example: biological fouling on the surface of the chip will reduce its detection sensitivity; biological fouling on the surface of biochemical separation membranes and water treatment membranes will block membrane pores and reduce membrane separation or purification efficiency. [0003] In view of the hazards of biofouling, in order to fundamentally solve the problem of fouling, experts and scholars at home and abroad have done a lot of research work. For example, people have designed an anti-fouling surface with protein enzymatic a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/40A01N25/08A01P3/00A01P1/00C09D5/14C08K9/04C08K3/04
Inventor 吴强李旭鹏杨凯
Owner 郑州欧姆康生物材料有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com