Preparation and application of polyunsaturated fatty acid containing rich conjugated linoleic acid

A technology of unsaturated fatty acid and conjugated linoleic acid, which is applied in the field of preparation of polyunsaturated fatty acids, can solve the problems of low utilization rate of raw materials, low extraction efficiency of conjugated linoleic acid, etc., achieves improved bioavailability, is beneficial to The effect of human digestion and absorption, improving storage stability

Inactive Publication Date: 2017-11-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main disadvantages of existing conjugated linoleic acid extraction methods are low raw material utilization rate and low extraction efficiency of conjugated linoleic acid
In addition, in many existing technologies, there is no report on the preparation of polyunsaturated fatty acids rich in conjugated linoleic acid using different milk fats as raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Extracting polyunsaturated fatty acids rich in conjugated linoleic acid from yak milk cream

[0035](1) Take an appropriate amount of solid yak milk cream and heat it at 37°C to obtain liquid yak milk cream; dissolve lipase Candida antarctica lipase B (Novozyme 435) with a neutral buffer solution (disodium hydrogen phosphate dihydrogen phosphate Sodium buffer, pH = 7.0) dissolved, according to the amount of enzyme 1000U g -1 Add it to the above-mentioned yak milk cream, react in a constant temperature magnetic stirrer at 50°C for 4 hours, remove lipase by suction filtration to terminate the reaction, cool to room temperature, and obtain a mixture containing mixed fatty acids;

[0036] (2) According to the ratio of 95% ethanol: urea = 3: 1, stir and mix evenly under the condition of 60 ° C, after the urea is completely dissolved, add the mixed fatty acid obtained in step (1) according to the ratio of urea: mixed fatty acid = 3: 1 A mixture of fatty acids, mix...

Embodiment 2

[0038] Example 2: Preparation of polyunsaturated fatty acid microcapsules rich in conjugated linoleic acid

[0039] (1) According to the ratio of gum arabic: casein: β-cyclodextrin = 3:1:4, prepare an aqueous wall material solution with a concentration of 5%-20% at 50°C and pH7.0;

[0040] (2) Take safflower oil as raw material, prepare unsaturated fatty acid according to the steps and parameters in Example 1, and make the polyunsaturated fatty acid rich in conjugated linoleic acid as capsule core, according to the mass ratio of wall material capsule core Add 7:3 to the above-mentioned wall material aqueous solution to make the solid content reach 40%, perform shear emulsification at 8000rpm with a high-shear emulsifier for 2min, and then homogenize at 45°C and 25MPa;

[0041] (3) Spray-dry the above-mentioned homogeneous emulsion with an inlet temperature of 230°C and an outlet temperature of 80°C to ensure continuous feeding. The feed rate is 300mL / h, the spray pressure is 0...

Embodiment 3

[0043] Example 3: Preparation of polyunsaturated fatty acid emulsion rich in conjugated linoleic acid

[0044] (1) Select whey protein and sodium caseinate to be used as emulsifier in combination, the ratio of the two is 1:1, and the total addition amount is 2%. Dissolve whey protein and sodium caseinate in water and keep the temperature at 50°C Stir evenly in a magnetic stirrer, hydrate overnight at 4°C, and then return to room temperature; dissolve carrageenan in water at 1% mass fraction, fully stir and hydrate, mix with emulsifier solution, and stir evenly;

[0045] (2) Using suet oil as raw material, prepare unsaturated fatty acid according to the steps and parameters in Example 1, add the prepared polyunsaturated fatty acid rich in conjugated linoleic acid to the mixed solution of emulsifier and polysaccharide at 50°C Saturated fatty acid, the addition amount is 10% of the mass of the mixed solution of emulsifier and polysaccharide, high-speed shear emulsification under ...

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Abstract

The invention discloses preparation and application of polyunsaturated fatty acid containing rich conjugated linoleic acid and belongs to the technical field of polyunsaturated fatty acid extraction and steady-state treatment of grease. A preparation method comprises the following steps: (1) preparing mixed fatty acid by utilizing an enzymolysis method; (2) enriching the polyunsaturated fatty acid by utilizing a urea-inclusion method; (3) enriching the conjugated linoleic acid by combining a super-critical CO2 extraction method and an alkali isomerization method to finally obtain the polyunsaturated fatty acid containing the rich conjugated linoleic acid, wherein the content of the conjugated linoleic acid reaches 90 percent or more and a product contains other polyunsaturated fatty acid at the same time so that the structure of the product is more reasonable. By adopting a method for preparing microcapsules or emulsion, the polyunsaturated fatty acid containing the rich conjugated linoleic acid is subjected to stabilization treatment, so that the storage stability of the product is improved and the storage period can reach 24 months; meanwhile, the bioavailability of the polyunsaturated fatty acid is improved and the polyunsaturated fatty acid is more easily digested and absorbed by human bodies.

Description

technical field [0001] The invention belongs to the technical field of polyunsaturated fatty acid extraction and steady-state treatment of oil, and in particular relates to the preparation and application of a polyunsaturated fatty acid rich in conjugated linoleic acid. Background technique [0002] Conjugated linoleic acid refers to the general term for a series of geometric isomers and positional isomer mixtures of linoleic acid containing conjugated double bonds. It is less abundant in nature and mainly exists in rumen animals, such as cattle, It also exists in sheep milk fat and meat products, vegetable oil and other non-ruminant animals, and the content in seafood is very small. A large number of research results have confirmed that conjugated linoleic acid has various biological activities such as inhibiting the formation of cancer and tumors, losing weight, anti-atherosclerosis, improving immune function, lowering cholesterol and promoting growth. At the same time, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B7/00C11C3/00C11C3/14A23D9/007A61K31/201A61K9/50A61K9/107A61P3/06A61P1/16
CPCA23D9/007A61K9/107A61K9/5052A61K31/201A61K47/36C11B7/00C11C3/00C11C3/14C12P7/6427Y02P20/54
Inventor 毛学英王婷婷赵丽丽刘泽朋
Owner CHINA AGRI UNIV
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