Preparation of glucose sensor based on graphene and graphene oxide composite material

A glucose sensor and composite material technology, which is applied in the direction of analyzing materials, electrochemical variables of materials, and material analysis through electromagnetic means, can solve the problems of reduced electrode stability, increased preparation complexity, and increased preparation costs, etc., to achieve improved stability performance, rapid electrochemical determination, and the effect of improving electrode stability

Active Publication Date: 2017-12-05
INST OF METAL RESEARCH - CHINESE ACAD OF SCI +1
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Problems solved by technology

The disclosed graphene oxide sensor usually adopts depositing gold or platinum nanoparticles on graphene to improve the conductivity of the electrode, such as: Chinese invention patent application (patent publication numbers are respectively: CN102507693A and CN104833714A), metal nanoparticles on graphene If the combination is not strong, the stability of the electrode will be reduced
At the same time, it will also increase the preparation cost and increase the complexity of the preparation, which is not conducive to practical application.

Method used

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  • Preparation of glucose sensor based on graphene and graphene oxide composite material
  • Preparation of glucose sensor based on graphene and graphene oxide composite material
  • Preparation of glucose sensor based on graphene and graphene oxide composite material

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preparation example Construction

[0035] The concrete steps of this preparation method are as follows:

[0036] (1) Pretreatment of the glassy carbon electrode: first, the glassy carbon electrode Use A1 with particle size of 1.0μm, 0.3μm and 0.05μm in turn 2 o 3 The suspension was polished to a mirror surface on the suede, and finally ultrasonically cleaned with absolute ethanol and double distilled water respectively, and set aside;

[0037] (2) Preparation of modified glassy carbon electrode:

[0038] a) Transfer the film-like graphene prepared by CVD to the surface of the polished glassy carbon electrode by bubbling or etching, which can significantly improve the electrical conductivity of the electrode. The substrate for growing graphene can be copper or It can be a sheet of metal such as nickel or platinum. In order to achieve ideal conductivity, the transfer times of the graphene film can be 1 to 5 times, and 2 times are commonly used.

[0039] Alternatively, transfer the foamed graphene prepared b...

Embodiment 1

[0045] The single-layer graphene grown on the copper foil by CVD is transferred from the substrate copper foil by the bubbling method (see Chinese invention patent, publication number CN102719877A), and fixed on the surface-cleaned glassy carbon electrode superior. First prepare the chitosan acetic acid solution (concentration of acetic acid solution is 2wt%) that concentration is 10mg / ml respectively, concentration is the chitosan acetic acid solution of the graphene oxide of 1mg / ml, the glucose oxidase phosphoric acid that concentration is 10mg / ml Salt buffer solution (the concentration of phosphate buffer solution is 0.1mol / L). Then, the glassy carbon electrode whose surface is modified with CVD graphene was modified, and 10 μl of graphene oxide chitosan acetic acid solution was added dropwise on the electrode. After the electrode was dried at room temperature, 10 μl of glucose oxidase phosphate buffered saline solution was added dropwise. After the electrode was dried, t...

Embodiment 2

[0047] The single-layer graphene grown on copper foil by CVD method was transferred from the substrate copper foil by bubbling method, and fixed on a diameter of on the glassy carbon electrode. In order to obtain a better conductive effect, the above operation is repeated once, that is, double-layer graphene is modified on the surface of the glassy carbon electrode. Other treatment methods and electrochemical experiments are the same as in Example 1. The experimental results show that the redox peak position and the redox peak current on the obtained cyclic voltammetry curve are basically the same as those in Example 1. It is proved that modifying a layer of graphene on the working electrode can obtain the ideal conductivity enhancement effect.

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Abstract

The invention belongs to the field of biosensors and specifically relates to a preparation method of a glucose biosensor based on a graphene and graphene oxide composite material. Graphene and graphene oxide are compounded as a modification layer of an electrode. Excellent conductivity of graphene is helpful for realizing direct electron transfer between glucose oxidase and the electrode; and a carboxyl group on graphene oxide can be combined with an amino group on glucose oxidase to achieve the effects of immobilizing glucose oxidase and raising electrode stability. A sensor with the above glucose oxidase-supported graphene modified electrode as a working electrode, a calomel electrode as a reference electrode and a platinum gauze electrode as a counter electrode can realize rapid electrochemical determination of glucose. The prepared graphene composite material is beneficial to immobilization of enzyme and also can achieve direct electron transfer with the electrode. The biosensor has high sensitivity, good stability, wide linear range and high anti-interference capability, and can be widely used for monitoring blood sugar and urine sugar for diabetic patients.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a method for preparing a glucose biosensor based on graphene and graphene oxide composite materials. Background technique [0002] Diabetes is a common and frequently-occurring disease with a high incidence in my country and the world. With the prolongation of the diabetes patient's illness time, if the metabolic disorder in the body is not well controlled, it can lead to chronic complications in tissues and organs such as eyes, kidneys, nerves, blood vessels and heart, and eventually blindness, lower limbs, etc. Gangrene, uremia, stroke or myocardial infarction, even life-threatening. So far, the number of diabetic patients in the country has exceeded 50 million, becoming the third "health killer" after cardiovascular and tumors. Therefore, the early detection and diagnosis of diabetes is of great significance for diabetic patients to control blood sugar and reduce the harm of diabet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
CPCG01N27/308G01N27/327G01N27/48
Inventor 英哲张睿杨卫东郝亚斌孙宝明宋纯曾尤成会明
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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