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Tylosin degrading bacterium and application thereof

A technology of tylosin and degrading bacteria, which is applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., and can solve the problems of tylosin environmental residues, etc.

Active Publication Date: 2018-01-09
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: how to provide a fast and efficient strain for degrading tylosin, so as to solve the problem of tylosin residues in the environment

Method used

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  • Tylosin degrading bacterium and application thereof
  • Tylosin degrading bacterium and application thereof
  • Tylosin degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The collection of embodiment 1 bacterial strain

[0017] Bacteria sample collection and processing

[0018] Collect the soil, weigh 5g of the soil, add it to a Erlenmeyer flask filled with 95mL of sterilized distilled water (250mL, containing glass beads), shake it on a constant temperature shaker at 30°C, 200r / min for 30min, take it out and let it stand still;

[0019] Strain Isolation

[0020] Take 100uL of soil extract, spread it on the beef extract peptone solid medium plate, and culture it under the condition of 30°C in shade for 2 days. The composition of beef extract-peptone solid medium is: 3g beef extract, 5g peptone, 5g sodium chloride, 18g agar, distilled water to 1000mL, adjust pH to 7.0-7.2 with 1mol / L sodium hydroxide, and then sterilize.

[0021] Strain purification

[0022] Take the single colony isolated on the plate, streak it on the beef extract peptone solid medium plate, further separate and purify; this step can be repeated 2 to 3 times until a ...

Embodiment 2

[0042] Example 2 Degradation Efficiency Determination of Tylosin Degrading Strains

[0043] Add 100 mg / L tylosin to the inorganic salt liquid medium, shake well, inoculate a single colony, and place it at 30°C, 150r / min, and culture in shading. Chromatographic conditions: Sunfire C18 chromatographic column (150mm×4.6mm, 3.5um, Waters, USA); mobile phase is 0.01% formic acid aqueous solution (A) and acetonitrile (B); flow rate is 1.0mL / min; column temperature is 40°C; The injection volume is 10uL; the detection wavelength is 274nm. Elution program: 0~6min, 80%~0%A, 20%~100%B; 6~7min, 0%~80%A, 100%~20%B; 7~10min, 80%A, 20% b. Take 1 mL of the liquid to be tested, add 5 mL of methanol, shake well, centrifuge at 10,000 r / min for 15 min, take the supernatant, filter it with a 0.22 μm syringe filter to obtain the filtrate, and then measure it with a high-performance liquid chromatograph, such as image 3 , according to the linear regression equation to calculate the degradation r...

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Abstract

The invention discloses a tylosin degrading bacterium and application thereof and belongs to the technical field of microbial degradation of antibiotic residues. The tylosin degrading bacterium LZ1 isachromobacter SP. and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 25, 2017, the preservation number is CGMCC No. 14071, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 courtyard, Beichen west road, Chaoyang District, Beijing. According to the tylosin degrading bacterium disclosed by the invention, soil is utilized as a screened initial raw material; an obtained bacterial strain and a prepared microbial inoculum can be directly applied to treating of waste of tylosin enterprises and possibly can be applied to renovating of peripheral soil and water environment which is polluted by tylosin.

Description

technical field [0001] The invention belongs to the field of microorganism degrading antibiotics, and in particular relates to a tylosin degrading bacterium and application thereof. Background technique [0002] Tylosin, also known as Tylosin or Tynon, was obtained by American scientists in 1959 in the study of a strain called Streptomycesfradiae (Streptomycesfradiae). Macrolide antibiotics. Tylosin is a white plate-like crystal, slightly soluble in water and alkaline. The molecular formula of Tylosin is: C 46 h 77 NO 17 , relative molecular mass: 916.1, chemical structural formula: [0003] [0004] Tylosin is a broad-spectrum antibiotic with special effect on mycoplasma, strong antibacterial effect on a variety of G+ bacteria, and inhibitory effect on some G- bacteria, Campylobacter, spirochetes and coccidia. [0005] At present, the environmental residue problem of tylosin has become one of the hot environmental issues that are widely concerned at home and abroad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/025C02F101/38
Inventor 李兆君孟应宏冯瑶文贻荣文贻宣
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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