Method for detecting ofloxacin in milk by combination of magnetic solid-phase microextraction and carbon quantum dot fluorescence sensibilization
A technology of carbon quantum dots and ofloxacin, applied in the field of magnetic solid-phase microextraction combined with carbon quantum dots fluorescence sensitization detection of ofloxacin in milk, to achieve fast separation speed, good specificity, and fast magnetic separation speed Effect
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Embodiment 1
[0027] Embodiment 1: the assay steps of ofloxacin in milk are as follows:
[0028] (1) Preparation of water-soluble nitrogen-doped fluorescent carbon quantum dots (N-CQDs): Weigh 10.0 g of polyethyleneimine liquid, add it to 90 mL of ultrapure water, and ultrasonically mix it thoroughly for 5 minutes, and transfer it to a polymer Tetrafluoroethylene reaction kettle, heated at 200°C for 5 hours, cooled naturally to room temperature, first filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500Da for 24 hours to obtain water-soluble nitrogen-doped fluorescence carbon quantum dots;
[0029] (2) The preparation methods of magnetic nanomaterials include:
[0030] Fe 3 o 4 Preparation: Take 2.05g of ferrous ammonium sulfate and 1.41g of ferric chloride, dissolve them in 50mL of deionized water, and heat them in a water bath to 80°C under the protection of nitrogen, add 5mL of ammonia water with a...
Embodiment 2
[0037] Embodiment 2: the assay steps of ofloxacin in milk are as follows:
[0038] (1) Preparation of water-soluble nitrogen-doped fluorescent carbon quantum dots: Weigh 12.0 g of polyethyleneimine liquid, add it to 90 mL of ultrapure water, ultrasonically mix it thoroughly for 10 min, and transfer it to a polytetrafluoroethylene reactor , heated at 250°C for 6 hours, cooled to room temperature naturally, filtered with a filter membrane with a pore size of 0.22 μm, and then dialyzed with a dialysis bag with a molecular weight cut-off of 3000 Da for 24 hours to obtain water-soluble nitrogen-doped fluorescent carbon quantum dots;
[0039] (2) The preparation methods of magnetic nanomaterials include:
[0040] Fe 3 o 4 Preparation: Take 2.20g of ferrous ammonium sulfate and 1.65g of ferric chloride, dissolve them in 50mL of deionized water, and heat them in a water bath to 75°C under nitrogen protection, then add 6mL of ammonia water with a concentration of 28% by volume and ...
Embodiment 3
[0046] Embodiment 3: the assay steps of ofloxacin in milk are as follows:
[0047] (1) Weigh 15.0g of polyethyleneimine liquid, add it to 90mL of ultrapure water, mix it thoroughly by ultrasonication for 8min, transfer it to a polytetrafluoroethylene reactor, heat at 220°C for 10h, and cool down to room temperature naturally , first filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3200 Da for 24 hours to obtain water-soluble nitrogen-doped fluorescent carbon quantum dots;
[0048] (2) The preparation methods of magnetic nanomaterials include:
[0049] Fe 3 o 4 Preparation: Take 2.50g of ferrous ammonium sulfate and 2.00g of ferric chloride, dissolve them in 50mL of deionized water, and heat them in a water bath to 85°C under the protection of nitrogen, add 7mL of ammonia water with a concentration of 28% by volume, and continue stirring React for 30 minutes, cool to room t...
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