A cell culture dish used for staining observation, and a cell culture staining and observing method
A technology of cell culture and petri dish, which is applied in the field of observation, cell culture dish, and cell culture staining. It can solve the problems of cells deviating from normal cell shape, poor control of strength, and floating slides, etc., so as to facilitate cell staining and follow-up observation. , Conducive to observation and photography, the effect of smooth surface
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] combine Figure 1 to Figure 5, a cell culture dish, comprising a culture dish body 3, a culture dish cover 2, a handle 1 is provided on one side of the culture dish body 3, the culture dish body 3 includes a base plate 33, and several base plate grooves 31 are provided on the base plate 33 The upper periphery of the bottom plate 33 is provided with a closed vertical sealing plate 32, the handle 1 is connected to the bottom plate 33, and the lower end surface of the culture dish cover 2 is matched with the upper end surface of the vertical sealing plate 32.
[0042] The vertical sealing plate 32 is provided with a sealing plate groove outside the upper part where it connects with the bottom plate 33 , and the sealing plate groove is an arc-shaped groove 34 or an inclined surface 35 from top to bottom. By providing a sealing plate groove on the outside of the vertical sealing plate 32, the thickness of the sealing plate groove portion becomes smaller, and the vertical sea...
Embodiment 2
[0050] A cell culture staining and observation method, specifically comprising the following steps:
[0051] 1) Culture medium preparation
[0052] Add 50-60 ml of fetal bovine serum (FBS), 3-5 ml of penicillin-streptomycin mixture, 0.01-0.02 g of type I collagen to every 450-500 ml of cell culture medium, and add appropriate types and concentration of growth factors or energy substances, and then filtered through a 0.22 micron filter membrane and then packed into sterilized centrifuge tubes, 40-45 ml per tube, stored at 4°C and used up within two weeks; before cell culture 0.5-1 Place the culture medium in a 37°C water bath to preheat;
[0053] 2) Cell culture
[0054] After 80-90% of the cells cultured in vitro adhere to the wall, add PBS solution to the culture bottle to wash once, then add 0.25% trypsin containing 0.05% EDTA and digest in a 37°C incubator for 3-5 minutes, then add Gently blow the culture medium into a single free cell, adjust the cell density to 2-5×10 ...
Embodiment 3
[0060] A cell culture staining and observation method, specifically comprising the following steps:
[0061] (1) Preparation of culture medium
[0062] Add 50 ml of FBS, 3 ml of penicillin-streptomycin mixed solution, 0.01 g of type I collagen, and 0.1 g of taurine to each 450 ml of DMEM / F12 solution, then filter through a 0.22-micron filter membrane and pack into a sterilized centrifuge. tubes, 40ml per tube, store at 4°C and use up within two weeks. Preheat the culture medium in a 37°C water bath 0.5 hours before cell culture.
[0063] (2) Bovine fat cell culture
[0064] After 80%-90% of bovine adipocytes cultured in vitro adhere to the wall, add 2 ml of PBS solution to the T25 culture flask to wash once, then add 1 ml of 0.25% trypsin (containing 0.05% EDTA) and place in a 37°C incubator Digest within 4 minutes, then add the culture medium and blow gently to form a single free cell, adjust the cell density to 3×104 cells / ml, add 4 ml of cell suspension in a special cell...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com