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Method for removing lignin through biological treatment of plant fibrous material hydrolysate

A plant fiber and biological treatment technology, applied in the field of comprehensive utilization of biomass, can solve the problems of high sugar loss rate, poor lignin removal selectivity, low removal efficiency of low molecular weight dissolved lignin, etc., so as to reduce the loss of sugar and improve the removal efficiency. rate effect

Active Publication Date: 2018-04-20
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the process of separation and purification of sugar in the hydrolyzate of existing plant fiber raw materials, when cationic polymers are used to remove lignin in the hydrolyzate, the removal efficiency of low molecular weight dissolved lignin is low and the removal selectivity of lignin is poor, which leads to sugar loss rate Big problem, the present invention provides a method for removing lignin by biological treatment of plant fiber raw material hydrolyzate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0043] A biological enzyme treatment method for plant fiber raw material hydrolyzate, the steps are as follows:

[0044] (1) The hydrolyzate of poplar wood self-hydrolyzed, the sugar content is 10.2g / L, the lignin content is 4.7g / L, the hydrolyzate is treated with horseradish peroxidase, the treatment conditions: the enzyme dosage is 10000U / L hydrolyzate, over The amount of hydrogen oxide is 8g / L hydrolyzate, the temperature is 30°C, the time is 6h, and the pH is 4.0.

[0045] (2) The hydrolyzed solution after the horseradish peroxidase treatment was allowed to stand for 8 hours, decanted to remove the precipitate, and the obtained upper layer hydrolyzed solution was treated with a cationic polymer. The cationic polymer had a molecular weight of 250,000 and a positive charge density of 7.0meq / L Cationic polymer polyallyl dimethyl ammonium chloride, cationic polymer dosage 200mg / L hydrolyzate, treatment temperature room temperature, pH 4.0, time 10 minutes. After the cationic ...

Embodiment 2

[0050] A method for biological treatment of plant fiber raw material hydrolyzate, the steps are as follows:

[0051] (1) The hydrolyzate after acid hydrolysis of maple wood, the sugar content is 18.2g / L, the lignin content is 8.6g / L, the hydrolyzate is first treated with horseradish peroxidase, the treatment conditions: the enzyme dosage is 15000U / L hydrolyzate, The amount of hydrogen peroxide is 10g / L hydrolyzate, the temperature is 25°C, the time is 10h, and the pH is 4.0.

[0052] (2) After horseradish peroxidase treatment, use polyethyleneimine with a molecular weight of 150,000 and a positive charge density of 6.5meq / g to treat with an amount of 150mg / L hydrolyzate, at room temperature, pH 4.0, for 15 minutes . After cationic polymer treatment, centrifuge for 15 minutes to remove the precipitate and obtain the purified hydrolyzate of the upper layer. After being treated with horseradish peroxidase-cationic polymer, the lignin removal rate was 58%, and the sugar loss rat...

Embodiment 3

[0055] A method for biological treatment of plant fiber raw material hydrolyzate, the steps are as follows:

[0056] (1) The hydrolyzate after self-hydrolysis of wheat straw, the sugar content is 8.7g / L, the lignin content is 3.7g / L, the hydrolyzate is first treated with horseradish peroxidase, the treatment conditions: the enzyme dosage is 15000U / L hydrolyzate , the amount of hydrogen peroxide is 10g / L hydrolyzate, the temperature is 30°C, the time is 12h, and the pH is 5.0.

[0057] (2) After horseradish peroxidase treatment, the polyamine with a molecular weight of 120,000 and a positive charge density of 6.6meq / g was used for treatment, with an amount of 160 mg / L hydrolyzate, at room temperature, pH 5.0, and for 20 minutes. After the cationic polymer was treated, it was left to stand for 6 hours, and the precipitate was removed by decantation to obtain the purified hydrolyzate of the upper layer. After being treated with horseradish peroxidase-cationic polymer, the lignin...

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Abstract

The invention relates to a method for removing lignin through biological treatment of plant fibrous material hydrolysate. According to the method, horse radish peroxidase treatment and then cationic polymer flocculation treatment are performed on the plant fibrous material hydrolysate, horse radish peroxidase treatment can ensure that low molecular weight dissolved lignin in the hydrolysate is polymerized into macromolecular lignin, and a part of lignin is removed through precipitation; after the lignin is subjected to horse radish peroxidase treatment, even if the part of the lignin which isnot separated out through precipitation is easier to remove in the subsequent cationic polymer flocculation treatment because of polymerization and increasing of molecular weight, and then cationic polymer treatment is adopted, so that residual lignin is further removed. The method can greatly improve the total removal rate of lignin through the combination of horse radish peroxidase treatment andcationic polymer treatment, and meanwhile can further obviously reduce the loss of sugar during the cationic polymer treatment.

Description

technical field [0001] The invention relates to a method for separating components of a hydrolyzate, in particular to a method for removing lignin by biological treatment of a plant fiber raw material hydrolyzate, and belongs to the field of comprehensive utilization of biomass. Background technique [0002] Hemicellulose is one of the most abundant natural polymers in nature, and lignocellulosic raw materials including wood and non-wood, such as bagasse, reed and various grasses contain a large amount of hemicellulose glycans. Hemicellulose has many uses, such as the production of furfural, hydroxymethylfurfural and other chemicals. High molecular weight hemicellulose can be used in the preparation of biomaterials such as food packaging films, hydrogels, surfactants and antibacterial materials. In addition, hemicellulose sugar can also be fermented to produce biofuels such as ethanol and methanol. Therefore, how to effectively utilize these hemicellulose glycans is of grea...

Claims

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Application Information

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IPC IPC(8): C08B37/14
CPCC08B37/0003
Inventor 李宗全王浩伟邱程龙高吉杰刘芹陈建松
Owner QILU UNIV OF TECH
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