Method for producing diosgenin by means of converting Tiegun rhizoma dioscoreae by aid of aspergillus oryzae

A technology of diosgenin and iron stick yam, which is applied in the biological field, can solve the problems of low transformation rate of Rhizopus oryzae, complicated method, long fermentation time and the like, and achieves the effects of short time, simple operation and low fermentation cost.

Inactive Publication Date: 2018-05-25
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many patents on the production of diosgenin by microbial transformation, and there are some defects, such as the low conversion rate of Rhizopus oryzae, ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of Aspergillus oryzae transformation medium: Add Tiegon yam powder as the main carbon source for the growth of Aspergillus oryzae, with a content of 3.00 g / L, wherein the Tiegon yam powder contains various saponins for the transformation of Aspergillus oryzae. Tetracycline is added in the medium to prevent infection with miscellaneous bacteria, and the content is 0.02g / L; vitamins, biotin, biological small molecules, inorganic salts, and minerals that can support the growth of Aspergillus oryzae are added in the medium; the medium contains 3.00 g / L sodium nitrate, 1.00g / L potassium dihydrogen phosphate, 0.50g / L magnesium sulfate heptahydrate, 0.50g / L potassium chloride, 0.01g / L iron sulfate heptahydrate, 3.00g / L iron rod yam powder, 0.02g / L tetracycline, 0.1g / L vitamin B 12 , 5.5g / L glycine, 0.5g / L cellulase, 0.5g / L α-amylase;

[0029] (2) Inoculate Aspergillus oryzae on the plate primary screening medium, culture in a biochemical incubator at 28°C for ...

Embodiment 2

[0034] (1) Prepare Aspergillus oryzae transformation medium. Add the iron stick yam powder as the main carbon source for the growth of Aspergillus oryzae, the content is 3.00g / L, wherein the iron stick yam powder contains a variety of saponins for the transformation of Aspergillus oryzae. Tetracycline is added in the medium to prevent infection with miscellaneous bacteria, and the content is 0.02g / L; vitamins, biotin, biological small molecules, inorganic salts, and minerals that can support the growth of Aspergillus oryzae are added in the medium; the medium contains 3.00 g / L sodium nitrate, 1.00g / L potassium dihydrogen phosphate, 0.50g / L magnesium sulfate heptahydrate, 0.50g / L potassium chloride, 0.01g / L iron sulfate heptahydrate, 3.00g / L iron rod yam powder, 0.02g / L tetracycline, 0.1g / L vitamin B 12 , 5.5g / L glycine, 0.5g / L cellulase, 0.5g / L α-amylase;

[0035] (2) Inoculate Aspergillus oryzae on the plate primary screening medium, culture in a biochemical incubator at 28...

Embodiment 3

[0040] (1) Prepare Aspergillus oryzae transformation medium. Add the iron stick yam powder as the main carbon source for the growth of Aspergillus oryzae, the content is 3.00g / L, wherein the iron stick yam powder contains a variety of saponins for the transformation of Aspergillus oryzae. Tetracycline is added in the medium to prevent infection with miscellaneous bacteria, and the content is 0.02g / L; vitamins, biotin, biological small molecules, inorganic salts, and minerals that can support the growth of Aspergillus oryzae are added in the medium; the medium contains 3.00 g / L sodium nitrate, 1.00g / L potassium dihydrogen phosphate, 0.50g / L magnesium sulfate heptahydrate, 0.50g / L potassium chloride, 0.01g / L iron sulfate heptahydrate, 3.00g / L iron rod yam powder, 0.02g / L tetracycline, 0.1g / L vitamin B 12 , 5.5g / L glycine, 0.5g / L cellulase, 0.5g / L α-amylase;

[0041] (2) Inoculate Aspergillus oryzae on the plate primary screening medium, culture in a biochemical incubator at 28...

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PUM

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Abstract

The invention belongs to the field of biotechnologies, and discloses a method for producing diosgenin by means of converting Tiegun rhizoma dioscoreae by the aid of aspergillus oryzae. The method includes inoculating the aspergillus oryzae into culture media with added Tiegun rhizoma dioscoreae powder, and carrying out culture at the fermentation temperature of 30 DEG C for the fermentation time of 6 d. The pH (potential of hydrogen) of the culture media is 6, and the inoculation amount of the aspergillus oryzae is 6%. The conversion rate of the Tiegun rhizoma dioscoreae powder which is converted into the diosgenin can reach 71.57% under the fermentation conditions. The method has the advantages that the diosgenin is high in yield, acidic organic wastewater pollutants can be prevented, environments can be protected in diosgenin production procedures, pollution can be prevented in the diosgenin production procedures, and the method is environmentally friendly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing diosgenin by transforming Aspergillus oryzae into yam. Background technique [0002] Diosgenin is an important raw material for the synthesis of cardiovascular disease drugs, steroid hormone drugs and contraceptive drugs. It has a wide range of applications, and more and more studies have shown that diosgenin can induce colon cancer and osteosarcoma cell apoptosis. , anti-hyperlipidemia, anti-platelet aggregation, anti-AIDS, anti-arteriosclerosis and other effects. At present, the acid hydrolysis method is mainly used to produce diosgenin in the industry. In this method, the glycoside bond of diosgenin is hydrolyzed with strong acid first, and then the diosgenin is extracted with an organic solvent. However, in the traditional process of production, a large amount of acidic wastewater is produced, which seriously pollutes the environment. In this ...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12R1/69
CPCC12P33/20
Inventor 钟先锋黄雯丽冯结铧黄桂东唐素婷吴子蓥
Owner FOSHAN UNIVERSITY
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