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Water-soluble fullerene nanomaterial and its preparation method and anti-oxidation application

A nanomaterial, fullerene technology, applied in the field of biomedicine, can solve the problems of destroying the π-π conjugated system, complex chemical synthesis process, easy introduction of organic reagents, etc., to achieve improved solubility, good physical and chemical stability , save the effect of complicated process

Inactive Publication Date: 2020-04-21
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more preparation methods are to modify multiple strong polar groups such as -OH, -COOH and -NH on the fullerene carbon cage through chemical reactions. 2 etc. to enhance its hydrophilicity, but the chemical synthesis process is complex, easy to introduce organic reagents, and chemical modification will destroy the π-π conjugated system of the fullerene carbon cage and reduce its biological activity

Method used

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  • Water-soluble fullerene nanomaterial and its preparation method and anti-oxidation application
  • Water-soluble fullerene nanomaterial and its preparation method and anti-oxidation application
  • Water-soluble fullerene nanomaterial and its preparation method and anti-oxidation application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Fullerene C 60 - Preparation of polydopamine-glutathione complex

[0031] 1) Take 100mg C 60 Add it into 30 mL of Tris-HCl buffer solution (pH=8.5) with a concentration of 10 mmol / L, and sonicate for 20 minutes to disperse evenly. Then, 200 mg of dopamine hydrochloride was added to the above system, and stirred for 10 h at 25° C. in the dark, at a speed of 1000 r / min.

[0032] 2) The above product was centrifuged at 8000r / min for 5 minutes, washed with distilled water and absolute ethanol, respectively, until the upper centrifuge was transparent, and the lower precipitate was placed in a vacuum drying oven for 24 hours, and the temperature was set at 40°C.

[0033] 3) Take the above dried fullerene C 60 - Add 100 mg of polydopamine product and 200 mg of glutathione (molecular weight: 600) to 20 mL of 0.1 mol / L sodium hydroxide solution, stir and react at 25° C. for 10 h, and rotate at 1000 r / min.

[0034] 4) After adding 15 mL of distilled water to the pro...

Embodiment 2

[0037] Example 2 Fullerene C 60 - Free radical scavenging performance test of polydopamine-glutathione complex.

[0038] For the water-soluble fullerene material obtained by the method, the free radical removal effect was measured by the spin trapping method (ESR). The specific operation is: for the free radicals generated by the reaction of hydrogen peroxide and ferrous ions, use DMPO (5,5-dimethyl-1-pyrroline-N-oxide) as a spin trapping agent to detect DMPO and hydroxyl free radicals The signal of the product DMPO-OH formed by the radical reaction.

[0039] 1) Blank test: the concentration of ferrous sulfate heptahydrate is 0.4mmol / L, the pH value of PBS is 7.4, the concentration of DMPO is 0.4mol / L, and the mass concentration of hydrogen peroxide is 5%. Take 50 μL of each of the above four solutions respectively Mix well, and finally add 50 μl of deionized water to the above mixed solution to carry out ESR test, and obtain the ESR signal of DMPO-OH in the blank test witho...

Embodiment 3

[0041] Example 3 Fullerene C 60 - Cytotoxicity and antioxidant properties tests of polydopamine-glutathione complexes.

[0042] 1 Fullerene C 60 - Toxicity test of polydopamine-glutathione complex on HEK-α of human epidermal cells

[0043] The cytotoxicity test is tested by the CCK-8 (Cell Counting Kit-8) kit, and the specific steps include:

[0044] 1) Firstly, the HEK-α of human epidermal cells was diluted to 5×10 with DMEM medium (modified Duchenne’s Eagle medium). 4 cells / ml, added to 96-well plate, 200 μl per well, incubated overnight;

[0045] 2) Change the medium and add different concentrations (25, 50, 100, 150 and 200 μg / mL) of fullerene C 60 - DMEM solution of polydopamine-glutathione complex;

[0046] 3) Incubate overnight, add CCK-8 indicator, and measure its light absorption value at 450nm with a microplate reader;

[0047] 4) Normalize the control group (control group) without drug addition, and calculate the cell viability of the drug addition group at di...

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Abstract

The invention discloses a water-soluble fullerene nano material. The water-soluble fullerene material is a fullerene C60-polydopamine-glutathione compound and is characterized in that fullerene and adopamine alkaline aqueous solution are subjected to mixing reaction directly to obtain a fullerene modified material of which the surface is attached with a polydopamine crosslinking composite layer,and the obtained fullerene modified material and water-soluble polypeptide glutathione with sulfhydryl radicals are subjected to Michael addition to obtain a water-soluble product. The invention alsodiscloses a preparation method and anti-oxidation application of the water-soluble fullerene nano material. The water-soluble fullerene nano material disclosed by the invention has good biocompatibility, good physical and chemical stability and mild and controllable preparation conditions; and as a scavenging agent and an antioxidant for free radicals in vivo, the water-soluble fullerene nano material shows very high potential values in the application fields of biomedicine and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a water-soluble fullerene nano material, a preparation method thereof and an anti-oxidation application. Background technique [0002] Fullerene is a new type of carbon-rich nanomaterial, which is an atomic cluster with a closed structure composed of different numbers of carbon atoms. Due to its unique structure and physical and chemical properties, it has very broad application prospects in the field of biomedicine. have I h Symmetrical C 60 The molecule is an outstanding representative of the fullerene family, which consists of 12 five-membered rings and 20 six-membered rings in a thirty-dodecahedron structure, with 30 conjugated double bonds, providing the opportunity to prepare different chemical or biological compounds, Known as the "Chemical pin cushion" in drug design. Since fullerene is a non-polar molecule, almost all life activities of biological syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/44A61K47/59A61K47/64A61P39/06A61K8/84A61K8/64A61K8/19A61Q19/08
CPCA61K8/19A61K8/64A61K8/84A61K33/44A61K2800/57A61Q19/08
Inventor 马艺函张孝焱陈小随
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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