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ICP-MS (inductively coupled plasma-mass spectrometry) detection kit for detecting elements in whole blood and application of detection kit

A technology of inductive coupling and plasma, applied in the field of medical biological detection, can solve problems such as pollution, element loss, and high detection results

Active Publication Date: 2018-06-22
SHANGHAI BIOTECAN PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of microwave digestion, due to the repeated use of quartz tubes or Teflon test tubes, it is easy to introduce contamination and cause high test results; moreover, the digestion process is cumbersome and requires high temperature heating, and elements may be lost; after digestion is completed, the cooling time is longer , which is not conducive to the detection of a large number of clinical samples

Method used

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  • ICP-MS (inductively coupled plasma-mass spectrometry) detection kit for detecting elements in whole blood and application of detection kit
  • ICP-MS (inductively coupled plasma-mass spectrometry) detection kit for detecting elements in whole blood and application of detection kit
  • ICP-MS (inductively coupled plasma-mass spectrometry) detection kit for detecting elements in whole blood and application of detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1: Detection of 32 elements in human whole blood

[0128] Embodiment 1 uses a kit to detect the content of 32 elements in whole blood, and the kit is as shown in Table 1:

[0129] Components of the kit described in Table 1 Example 1

[0130]

[0131]

[0132] Collect patient's fasting venous blood with heparin sodium anticoagulant tube, use kit among the present invention to 32 kinds of elements (comprising: lithium, beryllium, boron, magnesium, aluminum, phosphorus, calcium, titanium, vanadium, chromium, Manganese, iron, cobalt, nickel, copper, zinc, gallium, germanium, arsenic, selenium, rubidium, strontium, molybdenum, cadmium, tin, antimony, barium, platinum, mercury, thallium, lead, bismuth) for ICP-MS detection.

[0133] 1. Instrument and working parameters

[0134] The Agilent 7800ICP-MS instrument was used for elemental analysis. The instrument was equipped with an Agilent SPS4 autosampler with 240 samples. The basic parameter settings of the ins...

Embodiment 2

[0164] With higher concentrations of TritonX-100 and HNO 3 Diluent for pretreatment of whole blood

[0165] Under the instrument parameter of embodiment 1 and experimental condition, adopt 0.1% (v / v) TritonX-100-0.1% (v / v) HNO 3 The diluent is used to dilute the human whole blood sample and shake it well to obtain the sample solution to be tested. Use the standard solution, tuning solution and internal standard solution in the kit to establish a standard curve, then detect 32 elements on the whole blood quality control product and the whole blood sample to be tested, and perform 6 consecutive detections to calculate the RSD of each element , the RSD of each element is shown in Table 6. The test results show that the RSDs of various elements are significantly greater than the RSDs of the corresponding elements in Example 1. When the concentration of the diluent exceeds the concentration range of the diluent of the kit, for example, use 0.1% (v / v) TritonX-100-0.1% (v / v)HNO 3...

Embodiment 3

[0171] With a low concentration of TritonX-100 and a range of concentrations of HNO 3 Diluent for pretreatment of whole blood

[0172] Under the instrument parameter of embodiment 1 and experimental condition, adopt 0.01% (v / v) TritonX-100-0.06% (v / v) HNO 3 The diluent is used to dilute the human whole blood sample and shake it well to obtain the sample solution to be tested. Use the standard solution, tuning solution and internal standard solution in the kit to establish a standard curve, and then perform the detection of 32 elements on the whole blood quality control substance and the whole blood sample to be tested in sequence, 6 consecutive detections, and calculate the RSD of each element , the RSD of each element is shown in Table 7. During the experiment, it was found that precipitation gradually occurred in the diluted sample solution, and the RSDs of various elements were also significantly greater than the RSDs of the corresponding elements in Example 1. When the c...

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Abstract

The invention relates to an ICP-MS (inductively coupled plasma-mass spectrometry) detection kit for detecting elements in whole blood and an application of the detection kit and particularly providesa method and the kit for detecting elements in whole blood with CIP-MS. The method and the kit can detect the content of heavy metal elements in the whole blood of the human body, the content of microelements essential to the human body and the content of other elements, have the advantages that the sample dosage is small, pretreatment is simple, the cost is low, the detection accuracy and precision are high, the stability is high and the like and provide important reference for clinical heavy metal diagnosis and treatment and necessary nutrition support.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to an inductively coupled plasma mass spectrometry (ICP-MS) detection kit for elements in whole blood and an application thereof. Background technique [0002] Human body elements, especially the changes in the content of elements in the blood, are directly related to the physiological and pathological conditions of the human body. Determination of whole blood elements can be used as a reliable basis for health care, disease diagnosis and observation of curative effects. Human body elements such as magnesium, calcium, iron, manganese, copper, zinc, cobalt, vanadium, molybdenum, nickel, etc. are important components of biomacromolecules such as enzymes, hormones, and vitamins, and play important physiological and biochemical functions. There is a causal connection. Magnesium, calcium, iron, manganese, copper, zinc, selenium, chromium, boron, cobalt, vanadium, ru...

Claims

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Application Information

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IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 霍志远许骋黄永富荆莲艳闻颖俞东堃楼敬伟
Owner SHANGHAI BIOTECAN PHARMA