Broad-spectrum bacteriostatic Lactobacillus plantarum

A technology of Lactobacillus plantarum and strains, applied in the field of lactic acid bacteria, can solve the problems of insignificant inhibition effect and the like

Active Publication Date: 2018-06-29
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Baek et al found that Leuconostoc citreum and Weisella confuse could effectively inhibit the growth of Cladosporium sp YS1, Neurospora sp YS3 and Penicilliumcrustosum YS2 in rice cakes, and the antifungal substances were tested to be lactic acid and acetic acid; The lactic acid bacteria and the effect of inhibiting various Penicillium were studied, and it was confirmed that most of the Lactobacillus showed different degrees of inhibitory effects on Penicillium discolor and A. growth of P.commune and P.roqueforti, the inhibitory effect is relatively insignificant, and the inhibitory effect only occurs below pH 5.5

Method used

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  • Broad-spectrum bacteriostatic Lactobacillus plantarum
  • Broad-spectrum bacteriostatic Lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Isolation and screening of the strain MG-1

[0015] Take 10g of self-made natural fermented cabbage in Changsha City, dilute with sterile normal saline, mix it with 50℃ MRS agar medium containing calcium carbonate and pour the plate, culture at 38℃, select the large single colony of calcareous circle after 48h and repeat purification.

Embodiment 2

[0017] Example 2 Determination of antibacterial spectrum

[0018] Preparation of fermentation supernatant: After activation of the preserved strain, inoculate 2% into MRS liquid medium, incubate at 37°C for 18h, centrifuge at 12000r / min for 20min, filter and sterilize the supernatant through a 0.22μm filter, and store at 4°C .

[0019] Method: According to the Oxford Cup double-layer plate method, make indicator plates containing different indicator bacteria, the final concentration of bacteria in the upper semi-solid medium is 10 6 CFU / mL, fungus, yeast cell or spore 10 4 Pcs / mL, take 60μL of the fermentation supernatant of the strain and add it to the well, and cultivate under the optimum growth temperature of each indicator bacteria. Use sterile water as a negative control, and use hymexazol (diluted 1000 times as the original solution when used) as positive Control, when the negative control grows to the edge of the aperture, measure the diameter of the inhibition zone, and rep...

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Abstract

Broad-spectrum bacteriostatic Lactobacillus plantarum is disclosed, and the preservation number of the Lactobacillus plantarum MG-1 is CCTCC NO: M2018029. The strain MG-1 and a fermentation broth thereof have obvious inhibitory effects on bacteria and molds, can effectively inhibit grape Botrytis cinerea, grape Coniella diplodiella, Altenaria brassicae, Colletotrichum capsici, Diaporthe Kiwifruit,Capsicum southern blight pathogen, potato southern blight pathogen, Escherichia coli and Staphylococcus aureus, and has broad-spectrum bacteriostasis.

Description

Technical field [0001] The invention relates to a lactic acid bacterium with a broad spectrum and strong antibacterial activity, in particular to a plant Lactobacillus strain. Background technique [0002] As probiotics, lactic acid bacteria can produce some metabolites that can inhibit the growth of other microorganisms, such as bacteriocins, peptides, acid products (such as acetic acid, lactic acid and other organic acids), etc. These products have an inhibitory effect on spoilage microorganisms and pathogenic microorganisms. . Up to now, there are more than 300 kinds of lactic acid bacteria bacteriocins, which are produced by Lactobacillus, Lactococcus, Pediococcus, Leuconococcus, Enterococcus and other genus strains. Chemical preservatives widely used in the food industry seriously affect human health, and metabolites such as lactic acid bacteria bacteriocins and peptides are non-toxic, safe, and stable. Therefore, they are used as natural food preservatives to control certa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/25
CPCC12N1/20C12N1/205C12R2001/25
Inventor 易有金胡光耀夏菠曹熙
Owner HUNAN AGRICULTURAL UNIV
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