Preparation method of hydrophobic chitosan, product thereof, and use of product thereof for preparing hemostatic material
A technology of hemostatic material and chitosan, which is applied in the field of hydrophobic chitosan, preparation of hydrophobic chitosan, and preparation of hemostatic materials, can solve the problems of delayed wound healing, poor tissue adhesion, easy skin burns, etc., and achieves the preparation method Quick and easy, mild reaction process, improving the effect of long process
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preparation example 1
[0030] The preparation of preparation example 1 hydrophobic chitosan
[0031] (1) Dissolve chitosan with a viscosity-average molecular weight of 400,000 and a deacetylation degree of not less than 95% in an aqueous acetic acid solution with a concentration of 1% (ml / ml) until completely dissolved, and then perform suction filtration; wherein the aforementioned hydrophobic chitosan The weight to volume ratio of sugar and the aforementioned aqueous acetic acid solution is 1g:100ml;
[0032] (2) Add 0.3 g of lauric anhydride to the solution obtained in step (1), and react at a temperature of 55° C. for 2 to 24 hours, adjust the pH value to 11 with sodium hydroxide (NaOH), and filter the solution After washing to neutrality and drying, hydrophobic chitosan is obtained.
[0033] The aforementioned hydrophobic chitosan and general chitosan are further analyzed by infrared spectroscopy and nuclear magnetic resonance to obtain figure 1 and figure 2 .
[0034] figure 1 It is the ...
Embodiment 1
[0053] Example 1 In vitro coagulation test
[0054] (1) 1 g of hydrophobic chitosan with a degree of substitution of 5% to 20% is dissolved in 1% (ml / ml) acetic acid solution to prepare a hydrophobic chitosan acetic acid solution.
[0055] (2) Plasma preparation: Take 4.5ml of blood from rabbit vein, add 0.5ml of anticoagulant solution (38mg / ml), mix well, centrifuge at 1000r / min for 10min, and separate the plasma for later use.
[0056] (3) Determination of plasma recalcification time Take five test tubes, add 0.1ml of mixed plasma and normal saline to each tube, put them in a 37°C water bath and incubate for 1 minute, then add 0.1ml of calcium chloride solution (0.025mol / L) . After mixing, put it into a 37°C water bath, and start timing at the same time. After 1 minute, slowly tilt the test tube once every 15 seconds. Record self-added Ca 2+ From the time required for the fibrin to form and the liquid level not to move, calculate the average value of each test tube, whic...
Embodiment 2
[0062] The hemostatic effect test of embodiment 2 hydrophobic chitosan hemostatic sponge
[0063] (1) Hemostatic effect in vitro (rabbit ear arteriovenous hemorrhage model)
[0064] Nine male rabbits were randomly divided into three groups (hydrophobic chitosan hemostatic sponge, commercially available gelatin sponge, and medical gauze), with 3 rabbits in each group. Anesthetized with 10% (g / ml) chloral hydrate, injected intraperitoneally at a rate of 1-1.5ml / kg, depilated the ears after anesthesia, and performed wound treatment (2cm 2 ), in which the arteries and veins are transected, but the ear is not cut through. After the wound surface is filled with blood, three hemostatic materials including hydrophobic chitosan sponge, commercially available sponge, and medical gauze are used immediately, and then pressurized with a 50g weight. Record the hemostasis time (observed every 30s), and calculate the amount of bleeding.
[0065] The results are shown in Table 2 below. From...
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