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Activating-protein PeaT1 nanoparticles and application thereof to aspect of disease resistance and growth promotion of crops

A technology of nanoparticles and activating proteins, which is applied in the fields of application, plant growth regulators, plant growth regulators, etc., can solve the problems of unfavorable long-term storage and affecting long-term use, achieve good ecological and social benefits, and increase farmers Economic income, the effect of promoting seedling growth

Active Publication Date: 2018-07-24
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not conducive to long-term preservation, and also affects its long-term use

Method used

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  • Activating-protein PeaT1 nanoparticles and application thereof to aspect of disease resistance and growth promotion of crops
  • Activating-protein PeaT1 nanoparticles and application thereof to aspect of disease resistance and growth promotion of crops
  • Activating-protein PeaT1 nanoparticles and application thereof to aspect of disease resistance and growth promotion of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Synthesis and characterization of activator protein PeaT1 nanoparticles

[0017] (1) Synthesis of nanoparticles

[0018] Nanoparticles of silica were prepared by hydrolysis. Add 70mL deionized water, 0.8mL ammonia water, 15mL ether, 5mL ethanol to the flask, add 0.5g CTAB powder and stir at room temperature for 30min, then add 2.5mL TEOS and 0.1mL APES mixed solution dropwise, stir at room temperature for 4h, add 1mL 37 % hydrochloric acid to stop the reaction, centrifuge at 4200rpm for 12min, wash the precipitate with water and ethanol, and evenly disperse in ethanol to obtain the crude product of nanoparticles. Purify by gradient centrifugation, add 15mL 37% hydrochloric acid and 120mL ethanol, react at 70°C for 24h to remove the CTAB template, wash with centrifugation, disperse in PBS solution, add PeaT1 protein aqueous solution and magnetically stir for 1h to obtain nanoparticles loaded with activated protein PeaT1.

[0019] Nanoparticles loaded with act...

Embodiment 2

[0027] Example 2 Effects of Activating Protein Nanoparticles on Wheat Seed Germination and Seedling Growth

[0028] Spring wheat was selected as plant material, and the wheat was cultivated by hydroponics. First soak the seeds in 0.1% KMnO 4 solution for 20 minutes to disinfect the surface, then rinse with sterile water 3 times to wash. Afterwards, all seeds were divided into 4 groups and soaked overnight (approximately 10 hours). The first group (CK) used deionized water as a control, the second group (NPs) used blank nanoparticle aqueous solution, the third group (P) used protein aqueous solution, and the fourth group (PNPs) used activated protein nanoparticle aqueous solution . Afterwards, remove the seeds and wipe off any residual solution on the surface.

[0029]Cut a new filter paper into a circle with a diameter of about 5 cm, and soak it with deionized water. The seed arrangement is then placed in the middle of the paper and covered with plastic film. Afterwards,...

Embodiment 3

[0035] Example 3 The effect of activating protein nanoparticles on disease prevention and growth promotion of wheat seedlings

[0036] A biocontrol test of activating protein nanoparticles to control wheat root rot was carried out. Surface disinfection of wheat seeds with 0.1% KMnO 4 The solution was soaked for 30 minutes, divided into 5 groups and soaked overnight. B: blank group, soaked in sterile water without inoculation; CK: control group, soaked in sterile water; NPs: soaked in blank nanoparticle aqueous solution; P: soaked in PeaT1 protein aqueous solution; PNPs: activated protein nanoparticles Soak in aqueous solution. Except the first group (B), after soaking, the last four groups were planted after soaking with the spore suspension of Helminthiasis leaf blight for 30 minutes, and the planting method was the same as in Example 2. After two weeks of growth, the evaluation of the control effect of activin nanoparticles was carried out from the aspects of wheat morpho...

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Abstract

The invention discloses activating-protein PeaT1 nanoparticles and application thereof to the aspect of disease resistance and growth promotion of crops. The activating-protein PeaT1 nanoparticles arecharacterized in that silicon dioxide (SiO2) or amphiphilic copolymer mPEG-PLGA (Methoxy Poly(Ethylene Glycol)-(Poly(Lactic-co-Glycolic Acid) is adopted as nanocarriers, and the shape is roughly round, the diameter is about 200nm, the surface zeta potential is about -16.5mV, the encapsulation efficiency of activating protein PeaT1 in the nanocarriers is about 30.7%, and protein can be continuously released outwards with diffusion and degradation of the nanocarriers, so that the own immune systems of the crops such as wheat, rice, lettuce and cucumber and the like are continuously excited, theexpression and synthesis of own stress-resistant related substances of plants can be promoted, the growth and development can be promoted, the resistance can be improved and certain broad-spectrum controlling effect for diseases can be achieved. The pollution-free and high-efficiency activating-protein nanoparticles provided by the invention have the beneficial effects that the growth of the crops can be well promoted, the yield and the income can be increased and simultaneously the damage caused by disease to the crops can be controlled.

Description

technical field [0001] The invention relates to a novel activating protein PeaT1 nano particle and application thereof, belonging to the field of biotechnology. Background technique [0002] For a long time, crop yield increase and disease resistance mainly depend on the use of chemical fertilizers, pesticides and other reagents. The long-term use of these chemical reagents will not only pollute the environment, but also cause plant pathogens to develop resistance to fungicides, and even damage human health. The use of induced disease resistance produced by plants to prevent and control diseases, as well as the research and development of corresponding inducer pesticides, are developing rapidly and have broad prospects because of their green and high-efficiency characteristics. Compared with traditional pesticides and other methods, this new type of pesticide is a protein preparation, which is a new, safe and efficient "new concept" plant immune inducer, derived from natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/08A01N25/10A01N25/26A01N61/00A01P21/00A01P3/00
CPCA01N25/08A01N25/10A01N25/26A01N61/00Y02A40/10
Inventor 付玉明高寒刘红郭蓉
Owner BEIHANG UNIV