A method for preparing tissue engineering scaffold material by using degradable synthetic polymer material

Abstract

The invention discloses a method for preparing a tissue engineering scaffold material by using a degradable synthetic polymer material, which comprises the following steps: (1) forming a polymer sample through molding technology, and then placing it in a high-pressure container; (2) Use a supercritical fluid to replace the gas in the high-pressure container; (3) heat and pressurize the high-pressure container to saturate the high-molecular polymer sample in a high-temperature and high-pressure supercritical fluid atmosphere, and make the high-pressure container The molecular polymer sample is melted at a temperature higher than the critical temperature or critical pressure of the supercritical fluid, eliminating polymer crystallization; (4) cooling down to the foaming temperature; (5) rapid pressure release. The invention melts the crystal region of the high-molecular polymer through high temperature, eliminates polymer crystallization, solves the foaming problem of the linear linear high-crystallinity biological high-molecular polymer, and finally obtains a structure-controllable tissue engineering scaffold material.

Description

technical field [0001] The invention relates to a method for preparing a tissue engineering support material by using a degradable synthetic polymer material. Background technique [0002] In the prior art, tissue engineering scaffold materials refer to materials that can be combined with living cells of tissues and implanted into different tissues of organisms, and can replace the functions of tissues according to specific conditions. In order to proliferate and differentiate seed cells, it is necessary to provide a cell scaffold composed of biomaterials, which is equivalent to artificial extracellular matrix. Tissue engineering scaffold materials include: tissue scaffold materials for bone, cartilage, blood vessels, nerves, skin and artificial organs, such as liver, spleen, kidney, bladder, etc. [0003] At present, tissue engineering scaffold materials are classified according to their sources: (1) Natural biodegradable polymer materials, natural biodegradable polymer ma...

AI Technical Summary

Problems solved by technology

However, this spinning method has the following problems: (1) The prepared fiber scaffold does not have compression resistance; (2) The shear of the screw during the melt spinning process may cause material degradation, thereby reducing the mechanical properties of the finished product; (3) For polymers with low viscoelasticity, such as polyglycolide, it is impossible to prepare inorganic filler-modified spinning; (4) Electrospinning uses organic solvents to prepare polymer solutions, which may affect cell viability

Many high molecular polymers have a linear straight-chain structure, which leads to high crystallinity in the solid state, but low viscoelasticity in the molten state; therefore, the use of solid-state foaming will result in too small pore size of the foamed material and poor porosity. Very low, poor connectivity, making it unsuitable for tissue engineering applications

In the process of melt foaming, such as injection molding or extrusion foaming, the shear of the screw will cause the linear molecular chain to break, and the cells of the foamed material will collapse, which is not suitable for tissue engineering applications.

Images