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Preparation method of unpolished rice fermented raw solution for cosmetics and product of preparation method

A technology of cosmetics and brown rice, which is applied in the field of preparation of high-activity brown rice fermentation stock solution for cosmetics, can solve the problems of low extraction rate of active ingredients, high acidity, and difficulty in controlling the intermediate process, so as to shorten the fermentation cycle, improve fermentation efficiency, The effect of increased conversion efficiency

Active Publication Date: 2018-07-27
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are (1) the fermentation cycle of seed liquid fermentation is long and it is not suitable for scale-up, and it is difficult to realize large-scale production; (2) it is difficult to accurately control the fermentation metabolic pathway by using two-step fermentation process metabolism, and the product stability is difficult to guarantee; (3) Two-step fermentation using acetic acid bacteria also has the problem of high acidity
[0006] Chinese patent CN1957752A discloses "a brown rice fermented extract", in which the brown rice is fermented twice by yeast to obtain a fermented liquid. The disadvantage is that the first step of fermentation does not inoculate yeast, lacks fermentation metabolism control, and is easy to be infected with pathogenic bacteria. safety cannot be guaranteed
[0007] Chinese patent CN1386423A discloses "a fermented rice beverage and its preparation method". After gelatinization, the rice is inoculated with microorganisms and fermented for 1-5 days, and then filtered and sterilized to obtain the finished product. The disadvantage is that the raw material is peeled rice, and the rice bran is wasted. In addition, the active ingredients of its fermented products do not involve the content of GABA, which is related to the fact that the fermentation strains are purchased from the market and have no ability to convert GABA; meanwhile, the fermentation period is longer, up to 5 days
[0008] Chinese patent CN103705436 discloses "a kind of germinated brown rice herbal skin care product and its preparation method". Brown rice powder is gelatinized, enzymatically hydrolyzed and then compounded with herbal essence to obtain a finished product. The extraction rate is not high
[0012] 3. Most fermented rice products often use the evaluation methods in the food field to evaluate the fermentation process only from the physical characteristics such as appearance and state, while ignoring the most important guidance from the active ingredients of the product, especially the content of γ-aminobutyric acid The fermentation process and the lack of product efficacy data, which are very important factors in the field of cosmetics;
[0013] 4. The existing rice fermentation patents lack the intermediate control of fermentation, and most of them adopt multi-step fermentation. The disadvantage of multi-step fermentation is that the intermediate process is difficult to control, which makes the process difficult to scale up, and the product stability is difficult to guarantee. On the other hand, the fermentation cycle is generally long ,low efficiency

Method used

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  • Preparation method of unpolished rice fermented raw solution for cosmetics and product of preparation method
  • Preparation method of unpolished rice fermented raw solution for cosmetics and product of preparation method
  • Preparation method of unpolished rice fermented raw solution for cosmetics and product of preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of Yeast Fresh Cells

[0048] Pick Hansenula spp. strains from the test tube and inoculate them in 500mL sterile seed culture solution containing 4-8%wt of glucose, 0.5-1%wt of peptone, and 0.1-0.5%wt of dipotassium hydrogen phosphate, and the shaking table rotates at 150rpm. After culturing at 30°C for 13 hours, when it reached the logarithmic growth phase, it was inoculated again and expanded into 5L seed medium. After the second culture for 10 hours, it reached the logarithmic phase, and was obtained by centrifuging at 10,000 rpm for 30 minutes and discarding the supernatant.

Embodiment 2

[0050] (1) Weigh 10kg of brown rice and soak it in 20kg of water for 24 hours, then dry it at 60°C for 10 hours, and grind it into brown rice flour with a moisture content of 5.2%;

[0051] (2) Take 8.5kg of brown rice flour in step (1) and add 100L of water to mix it into rice milk, then add 100mL of α-amylase solution and α-glucoamylase solution in total, enzymatically hydrolyze, measure the glucose content every 0.5h, until no longer When changing, the enzymatic hydrolyzate of rice milk was obtained, and its residual sugar content was 58.5g / L;

[0052] (3) Add 5g / L bean cake powder to the above rice milk enzymatic hydrolysis solution as a nitrogen source, 1g / L dipotassium hydrogen phosphate as a culture medium, insert the yeast cells in Example 1, and add 1g biotin at the same time , add 55g of glutamic acid powder in the middle to keep the pH between 5.0-5.5, ferment at 30°C for 20 hours, after there is no residual sugar, the fermentation ends, and the fermentation liquid ...

Embodiment 3

[0055] (1) Weigh 3kg of brown rice and immerse it in 4.5kg of water for 18 hours, then dry it at 80°C for 5 hours, and grind it into brown rice flour with a moisture content of 6.2%;

[0056] (2) Take 2.2kg of brown rice flour in step (1) and add 30L of water to mix it into rice milk, then add 25ml of α-amylase solution α-glucoamylase solution, enzymatically hydrolyze, measure the glucose content every 0.5h, until no change , the rice milk enzymatic hydrolyzate was obtained, and its residual sugar content was 63.1g / L;

[0057] (3) Add 5g / L yeast powder to the above-mentioned rice milk enzymolysis solution as a nitrogen source, 1g / L dipotassium hydrogen phosphate as a culture medium, insert the yeast cells in Example 1, and add 0.3g tobacco Add 15g of glutamic acid powder in the middle to keep the pH between 5.0-5.5, and ferment at 32°C for 16 hours. After no residual sugar, the fermentation ends and the fermentation liquid is obtained;

[0058] (4) The fermentation broth was ...

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Abstract

The invention provides a preparation method of an unpolished rice fermented raw solution for cosmetics. The preparation method comprises the following steps: after accelerating germination of unpolished rice, crushing the unpolished rice into unpolished rice powder; then preparing the unpolished rice powder into rice milk; after carrying out enzymolysis, adding saccharomycetes and fermenting; removing impurities and bacteria from a fermentation solution to obtain a finished product. According to the preparation method provided by the invention, a fermentation process can be controlled and active substances in the unpolished rice is efficiently enriched within extremely short time; the preparation method has the advantages of short period, controllable process, easiness for amplifying a technology and stable product; the solution has high content of active substances and a good effect, is easy to absorb, is safe and healthy and meets the requirements of cosmetic raw materials.

Description

technical field [0001] The invention relates to the field of cosmetic raw materials, in particular to a method for preparing a high-activity brown rice fermented stock solution for cosmetics. Background technique [0002] my country is a big country of rice production and consumption, with an annual output of more than 200 million tons. Brown rice is different from ordinary polished rice in that the chaff has not been removed. Brown rice not only contains the endosperm, but also retains the bran layer that was removed during the whitening process. , and the bran layer is the main nutrient enrichment place in rice, which contains more active ingredients, such as vitamins, phytosterols, phenolic acids, γ-aminobutyric acid, etc., of which γ-aminobutyric acid has the highest content. γ-aminobutyric acid is an inhibitory neurotransmitter in the central nervous system. It has extremely important physiological functions. It has good anti-oxidation and whitening effects on human skin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61K8/44A61Q19/00A61Q19/08A61Q19/02
CPCA61K8/44A61K8/97A61K2800/85A61Q19/00A61Q19/02A61Q19/08
Inventor 陆震魏玉洁孙元军石艳丽
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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