Red monascus and application to preparation of fat-reducing medicines

A Monascus, red technology, applied in the field of traditional Chinese medicine (materials), can solve the problems of failing to meet national or foreign standards, high citrinin content, etc., to achieve no pollution, suitable for large-scale production, and high quality stability Effect

Active Publication Date: 2018-07-31
FUJIAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that Monacolin, the main functional component of Monascus purple, produces little or no substances in the series of monacolin, while the content of citrinin is relatively high, and most of them fail to meet the national or foreign standards.

Method used

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  • Red monascus and application to preparation of fat-reducing medicines
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  • Red monascus and application to preparation of fat-reducing medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Identification of Monascus ruberus FWB13

[0032] A Monascus strain isolated from Monascus which can produce MK but not produce citrinin was identified mainly by morphological and gene sequence analysis methods.

[0033] (1) Morphological identification of Monascus ruberus FWB13

[0034] 1. Medium

[0035] (1) Wort agar medium (Wa): 10 Bx wort juice 1000mL, agar 15g.

[0036](2) Saccharomyces tsarzia extract agar medium (CYA): NaNO 3 33.0g, K 2 HPO 4 1.0g, KCl 0.5g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, yeast extract 5.0g, glucose 20g, agar 15g, add distilled water to 1000 mL.

[0037] (3) Glycerol nitrate agar medium (G25N): add 25% glycerol (w / w) to CYA medium.

[0038] (4) Potato dextrose agar medium (MEA) medium: 20g malt extract, 1.0g peptone, 20g glucose, 15g agar, add distilled water to 1000 mL.

[0039] (5) Potato dextrose agar medium (PDA) medium: Peel 200g potatoes, cut into small pieces, add 1000mL distilled water and boil for 20min...

Embodiment 2

[0077] Embodiment 2, the preparation of Monascus ruberus FWB13 solid-state fermentation product and the detection of MK and citrinin

[0078] (1) Medium and culture conditions

[0079] 1. Slant medium and slant culture: 2% soluble starch, 6% maltose, 2% peptone, and 2% agar. Put the slant medium into a test tube, sterilize it, and place it on an inclined plane to cool. Monascus red fungus FWB13 was inoculated on the slant medium, and cultured at 30°C for 5 days until the hyphae covered the slant.

[0080] 2. Seed liquid culture medium and shake flask culture: rice flour 2%, glucose 2.0%, peptone 2.5%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.1%, sodium nitrate 0.5%, according to the size of the container, fill 1 / 5 liquid medium, sterilized and cooled. Wash the slant culture fluid obtained after washing the slant spores with 10 mL of sterile water, and adjust the spore concentration to 10 6 cfu / mL, inoculum size 5%, cultured on a rotary shaker at 30°C and 1...

Embodiment 3

[0089] Embodiment three, zoological function evaluation

[0090] (1) Test materials

[0091] 1. Test drug: sample Aa, sample Ab, sample Ac, sample Ad, sample Ae. Wherein except that the Ac sample is the self-made red yeast rice sample of Example 2 of the present invention, all the others are red yeast rice raw materials with lipid-lowering function sold on the market.

[0092] 2. High-fat feed: Add 20.0% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium cholate, appropriate amount of casein, calcium hydrogen phosphate, stone powder, etc. to the maintenance feed. Except for crude fat, the moisture, crude protein, crude fat, crude fiber, crude ash, calcium, phosphorus, calcium: phosphorus of the model feed all reached the national standard for maintenance feed. Mix well, press and bake into strips, freshly prepared within 1 week, and stored at 4°C.

[0093] 3. Serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein...

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Abstract

The invention belongs to the technical fields of pharmacy, microorganisms and fermentation thereof and provides red monascus and application to preparation of fat-reducing medicines. The bacterium isthe red monascus (M.ruber) FWB13, and is registered and collected in General Microbiological Center of China Microbiological Culture Collection Management Committee on March 6th, 2017, and the collection number is CGMCCNo.13685. Proved by experiments, after by fermentation culture of red monascus FWB13, the monascus traditional Chinese medicine with high MK content can be obtained, and no citrininis detected out (the detection limit is 16mu g/kg), so that the market prospect is good.

Description

technical field [0001] The present invention relates to the technical fields of pharmacy, microorganism and fermentation thereof, in particular to Monascus ( Monascus ) strains, traditional red yeast rice Chinese medicine process, Chinese medicinal materials with blood lipid-lowering function, traditional Chinese medicine decoction pieces and their capsules and tablets Chinese medicine [hereinafter referred to as Chinese medicine (material)]. Background technique [0002] Red yeast rice is cultivated by inoculating Monascus fungus on rice. According to its function, it is divided into three types: brewing yeast, color yeast and functional red yeast rice. It has a history of more than one thousand years and is China's "national song". It is the birthplace and main producing area of ​​red yeast rice. Monacolin K, MK, an inhibitor of cholesterol synthesis in red yeast rice, was obtained from the Monascus red fungus of the genus Monascus ( M. ruber ) No.1005 strain was found ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/062A61P3/06C12R1/645
CPCA61K36/062A61P3/06C12N1/14C12N1/145C12R2001/645
Inventor 林风吴丽云李志强刘颖
Owner FUJIAN INST OF MICROBIOLOGY
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