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A kind of prebiotic composition and its preparation method and application

A composition and prebiotic technology, applied in the field of prebiotic composition and its preparation, can solve the problem of relatively few researches on the deeper regulation of intestinal flora, and achieve the promotion effect and utilization rate, rich source of raw materials, inhibiting Effects of Enteric Pathogen Growth

Active Publication Date: 2020-10-16
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the perspective of probiotics or probiotic health drinks studied in the prior art, research in this field mainly focuses on using xylooligosaccharide as a raw material formula to regulate conventional flora such as Bifidobacterium and Lactobacillus in the human intestinal flora. , there are relatively few studies on the deeper regulation of intestinal flora. Therefore, the development of prebiotics with rich raw materials, lower cost and stronger functions is of great significance to promote the health of intestinal flora in humans and animals. Human and animal use of prebiotics offers wider market options

Method used

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  • A kind of prebiotic composition and its preparation method and application
  • A kind of prebiotic composition and its preparation method and application
  • A kind of prebiotic composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of prebiotic composition

[0022] Weigh 85g of powdered xylan and 15g of powdered bamboo leaf flavone extract, mix and stir them evenly to obtain a prebiotic composition.

[0023] Source of xylan: Vetec company.

[0024] Bamboo leaf flavonoid extract: Hangzhou Youmet Technology Co., Ltd.

Embodiment 2

[0025] Example 2 investigates the regulating effect of the prebiotic composition of the present invention on human intestinal flora

[0026] Test method: In vitro simulated human intestinal glycolysis test

[0027] Fresh feces were collected from 3 volunteers. The volunteers were required to eat a normal diet, have no digestive diseases, and had not taken antibiotics for at least 3 months. An equal amount of feces was pooled from each volunteer and immediately stored in anaerobic containers. Weigh 100g of feces sample, add 5 times the volume of feces weight in pre-reduced sterile saline (NaCl 8.5g / L, cysteine ​​hydrochloride 0.5g / L) into the anaerobic workstation, immediately tighten the lid, and Vibrate fully on the vortex instrument, filter it into another sterile sample bottle with 4 layers of sterile gauze on the ultra-clean bench, and immediately tighten the cap to obtain a 20% (w / v) stool dilution.

[0028] Preparation of fermentation medium containing prebiotic compos...

Embodiment 3

[0037] 1. Detection method:

[0038] (1) Fecal microbial diversity detection: Fast DNA SPIN kit (MP Biomedicals, USA) was used to extract the DNA of the fermentation culture collected at 0h and 48h, and then commissioned Shanghai Passino Technology Co., Ltd. to analyze the culture. Microbial composition was analyzed.

[0039] (2) The concentrations of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid in short-chain fatty acids (SCFA) were detected by GC-MS method. The specific steps are: accurately measure 250 μL of the zymolysis culture supernatant into a 2 mL glass centrifuge tube. Add 750μL 0.005M NaOH aqueous solution, and 50μL DL-2-methylbutyric acid, shake and mix for 2min, let stand at 4°C for 2h, shake and mix again for 2min, centrifuge (4°C, 12 000rpm, 20min); take 500μL supernatant Transfer to a clean 15mL screw cap centrifuge tube, add 300μL ddH2O, 500μL isopropanol / pyridine solution (3:2, v / v), 100μL PCF solution for deriv...

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Abstract

The invention discloses a prebiotic composition. The prebiotic composition is prepared from the main effective components of xylan and flavone according to a mass ratio of 7:9 to 3:1. A preparation method comprises the following step of uniformly mixing the xylan and the flavone, so as to obtain the prebiotic composition. Proofed by experiment, the prebiotic composition has the advantages that theprebiotic composition can be applied to prepare medicines or health-care products for regulating intestinal flora of human or animals, medicines or health-care products for inhibiting fusobacterium in the intestinal flora of the human or animals, and medicines or health-care products for promoting proliferation of phascolarctobacterium in the intestinal flora of the human or animals; the cost islow, the preparation method is simple, and the intestinal flora structure of the human or animals can be obviously regulated; the prebiotic composition is especially suitable for obviously inhibitingthe growth of the fusobacterium and the proliferation of the phascolarctobacterium; compared with the common prebiotic composition, the better health-care function and higher use value can be realized.

Description

technical field [0001] The invention relates to the technical field of compositions, in particular to a prebiotic composition and its preparation method and application. Background technique [0002] The gastrointestinal tract of humans and animals is home to a wide variety and an astonishing number of microorganisms. The metabolic activities of intestinal microbes interact with the host, adapt to each other, and form a specific intestinal micro-ecosystem after long-term natural selection. All healthy adults share many of the same gut microbial species, a core microbiome. Intestinal microecology is closely related to human health. The imbalance of intestinal flora can lead to various diseases in the human body. Maintaining a normal intestinal flora structure is a necessary condition for maintaining the health of the host. Food functional factors can carry out body defense, disease prevention and health recovery, etc. by regulating the intestinal microecological balance. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/899A61P1/00A61P31/04A23L33/125A23L33/105
CPCA23L33/105A23L33/125A23V2002/00A61K31/715A61K36/899A61P1/00A61P31/04A61K2300/00A23V2200/3202A23V2200/30A23V2250/2116A23V2250/284
Inventor 黄菊青徐庆贤林斌郑怡官雪芳钱蕾张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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