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Preparation and application of a polymer nanoparticle capable of ratiometric fluorescence detection of hypochlorous acid

A technology for the detection of hypochlorous acid and fluorescent polymers, used in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve problems such as limited applicability, reduced feasibility, etc. The effect of convenient post-processing and stable dispersion

Active Publication Date: 2020-07-31
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when it comes to small molecule sensors, its unique defects cannot be ignored. First, most small molecule sensors work in pure organic solvents or mixed solvents, because the biological toxicity of organic solvents limits its applicability; secondly, Small molecule sensors in water can cause aggregation-induced fluorescence quenching (ACQ) due to π-π interactions between them, further limiting its applicability.
This also leads to a reduction in the feasibility of using this type of sensor in actual detection.

Method used

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  • Preparation and application of a polymer nanoparticle capable of ratiometric fluorescence detection of hypochlorous acid
  • Preparation and application of a polymer nanoparticle capable of ratiometric fluorescence detection of hypochlorous acid
  • Preparation and application of a polymer nanoparticle capable of ratiometric fluorescence detection of hypochlorous acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing polymer nanoparticles with ratiometric fluorescence detection of hypochlorous acid, comprising the following steps:

[0038] (1) Esterification to synthesize 4-(1,2,2-triphenylvinyl)phenyl undecylenate.

[0039] Weigh 0.2g 4-(1,2,2-tristyryl)phenol (TPE-OH), 0.4g n-undecylenoyl chloride, 0.25g N,N-diisopropylethylamine (DIEA), in Dissolve TPE-OH and DIEA with 10mL of dichloromethane in a 25mL single-necked bottle, then dissolve n-undecyl chloride with 1mL of dichloromethane, slowly add the dissolved n-undecyl chloride into the single-necked bottle with a syringe, and react at room temperature for 24 hours After the reaction, the desired product was obtained by silica gel column chromatography separation method, and then the solvent was removed with a rotary evaporator, and after vacuum drying, the desired 4-(1,2,2-triphenylethenyl)phenyl Undecylenate (Product 1).

[0040] (2) Miniemulsion polymerization to prepare fluorescent polymer nanoparticl...

Embodiment 2

[0045] A method for preparing polymer nanoparticles with ratiometric fluorescence detection of hypochlorous acid, comprising the following steps:

[0046] (1) Esterification to synthesize 4-(1,2,2-triphenylvinyl)phenyl undecylenate.

[0047] Weigh 0.2g 4-(1,2,2-tristyryl)phenol (TPE-OH), 0.46g n-undecylenoyl chloride, 0.3g N,N-diisopropylethylamine (DIEA), in Dissolve TPE-OH and DIEA with 10mL of dichloromethane in a 25mL single-necked bottle, then dissolve n-undecyl chloride with 1mL of dichloromethane, slowly add the dissolved n-undecyl chloride into the single-necked bottle with a syringe, and react at room temperature for 24 hours After the reaction, the desired product was obtained by silica gel column chromatography separation method, and then the solvent was removed with a rotary evaporator, and after vacuum drying, the desired product 4-(1,2,2-triphenylethenyl)benzene could be obtained Undecylenate (Product 1).

[0048] (2) Miniemulsion polymerization to prepare fluo...

Embodiment 3

[0053] A method for preparing polymer nanoparticles with ratiometric fluorescence detection of hypochlorous acid, comprising the following steps:

[0054] (1) Esterification to synthesize 4-(1,2,2-triphenylvinyl)phenyl undecylenate.

[0055] Weigh 0.2g 4-(1,2,2-tristyryl)phenol (TPE-OH), 0.6g n-undecylenoyl chloride, 0.4g N,N-diisopropylethylamine (DIEA), in Dissolve TPE-OH and DIEA with 10mL of dichloromethane in a 25mL single-necked bottle, then dissolve n-undecyl chloride with 1mL of dichloromethane, slowly add the dissolved n-undecyl chloride into the single-necked bottle with a syringe, and react at room temperature for 24 hours After the reaction, the desired product was obtained by silica gel column chromatography separation method, and then the solvent was removed with a rotary evaporator, and after vacuum drying, the desired product 4-(1,2,2-triphenylethenyl)benzene could be obtained Undecylenate (Product 1).

[0056] (2) Miniemulsion polymerization to prepare fluor...

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Abstract

The invention discloses preparation and application for polymer nanoparticles capable of detecting hypochloric acid by ratio fluorescence. The polymer nanoparticles use methyl methacrylate, 2-aminoethyl methacrylate hydrochloride, and 4-(1,2,2-tristyryl) benzene butyl undecylenate as a monomer, use rhodamine B isothiocyanate as a response group, and are prepared from the combination of a one-stepfine emulsion polymerization method and particle surface modification technology. Compared with the prior art, the polymer nanoparticles of the invention can perform high sensitivity and high selectivity detection on the hypochloric acid in water, have a simple synthesis route, are convenient to use, are suitable for amplification synthesis and practical production application, and have a great application prospect in the fields of bioanalytical chemistry, cell biology, medical science and environment detection.

Description

technical field [0001] The invention relates to the technical field of material preparation and biological and environmental ion detection, in particular to the preparation of a polymer nanoparticle that can be used for the recognition function of hypochlorous acid, and the application of the polymer nanoparticle to detect hypochlorous acid in water. Background technique [0002] Reactive oxygen species (ROS) is a very important species that widely exists in biological cells, and it plays a very important role in signal transduction, differentiation, migration and cellular immunity in biological cells. However, ROS all have the common characteristics of high activity, low content, and uneven distribution in organisms. How to explore their functions in organisms has become particularly important. In addition, HClO is used in the defense system by various organisms in nature as a bacterial killer. As a highly effective bactericidal weapon, its mechanism is that bacteria must ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/14C08F220/34C08F220/16C08F8/30G01N21/64
CPCC08F8/30C08F220/14G01N21/643C08F220/34C08F220/16
Inventor 陈建薛明菊王宏余茂林任俊宇刘杰张培盛
Owner HUNAN UNIV OF SCI & TECH
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