Biopesticide preparation method
A technology of biological pesticides and strains, applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of poor control effect, easy death and inactivation of microorganisms, and short storage time. To achieve the effect of improving antibiotic production, high insecticidal activity, and promoting cell division
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[0023] A preparation method of biopesticide, the preparation method comprises the steps of:
[0024] (1) Take Streptomyces venezuela and inoculate 2~5% of the inoculum into the activation medium, and culture it at 25~28°C for 5~7 days to obtain a culture. Take the culture and add normal saline at a mass ratio of 1:8. Cultivate at 200r / min, 25~28°C for 2~3h, filter with sterile absorbent cotton, take the filtrate and mix it with sterile water at a mass ratio of 1:6~8 to obtain a mixture;
[0025] (2) Take the mixed solution and place it at a distance of 30cm from a 15W ultraviolet lamp to irradiate for 40~45s to obtain the pretreated bacterial solution. Take the pretreated bacterial solution and dilute the pretreated bacterial solution with normal saline to 10 -6 According to the dilution level, the dilution was obtained, and the dilution was inoculated into the mutagenesis medium at an inoculation amount of 2-4%, and cultured at 25-28°C in the dark for 3-5 days to obtain seed ...
Embodiment 1
[0030] Streptomyces venezuela: Preserved on a slope at 4°C in the laboratory.
[0031] Ivermectin: purchased from Zhoukou Jinshi Chemical Co., Ltd.
[0032] Activation medium: in parts by mass, take 20 parts of soluble starch, 1 part of potassium nitrate, 0.3 parts, 0.3 parts of magnesium sulfate, 0.008 parts of ferrous sulfate, 1000 parts of water, pH 7.2, sterilize at 121°C for 20 minutes, that is have to.
[0033] Mutagenesis medium: in parts by mass, take 20 parts of soluble starch, 1 part of potassium nitrate, 0.3 parts, 0.3 parts of magnesium sulfate, 0.008 parts of ferrous sulfate, 3 parts of lithium chloride, 20 parts of agar, 1000 parts of water, pH 7.2, sterilized at 121°C for 20 minutes, ready to use.
[0034] Fermentation medium: in parts by mass, take 20 parts of soybean cake powder, 8 parts of glucose, 10 parts of starch, 0.3 parts of dipotassium hydrogen phosphate, 2 parts of calcium carbonate, 1000 parts of distilled water, pH 8.0, sterilize at 121°C for 20 m...
Embodiment 2
[0042] Streptomyces venezuela: Preserved on a slope at 4°C in the laboratory.
[0043] Ivermectin: purchased from Zhoukou Jinshi Chemical Co., Ltd.
[0044] Activation medium: in parts by mass, take 23 parts of soluble starch, 2 parts of potassium nitrate, 0.4 parts, 0.4 parts of magnesium sulfate, 0.009 parts of ferrous sulfate, 1000 parts of water, pH 7.3, sterilized at 121°C for 20 minutes, namely have to.
[0045] Mutagenesis medium: in parts by mass, take 23 parts of soluble starch, 2 parts of potassium nitrate, 0.4 parts, 0.4 parts of magnesium sulfate, 0.009 parts of ferrous sulfate, 4 parts of lithium chloride, 23 parts of agar, 1000 parts of water, pH 7.3, sterilized at 121°C for 20 minutes, ready to use.
[0046] Fermentation medium: in parts by mass, take 23 parts of soybean cake powder, 10 parts of glucose, 13 parts of starch, 0.4 parts of dipotassium hydrogen phosphate, 3 parts of calcium carbonate, 1000 parts of distilled water, pH 8.0, sterilized at 121°C for ...
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