Preparation method and application of a fluorescent probe based on self-assembled vesicles
A technology of vesicles and fluorescence, which is applied in the field of nanomaterials, can solve the problems of complex preparation reaction process of fluorescent vesicles, unfavorable production and application, adverse effects on organisms, etc., achieve good application prospects, reduce energy consumption, and easy to obtain raw materials Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] This example prepares fluorescent vesicles by the following method
[0044] Butyric acid with a concentration of 100mM was dissolved in a mixed solvent of water and methanol, wherein the mass fraction of methanol was 50%, and then acetate buffered salt was added to adjust the pH of the solution to be 5. After the solution was mixed evenly, it was placed under the light of 400nm wavelength , the stirring rate was 200r / min for the reaction, and the fluorescent vesicles were obtained after 12 hours of reaction.
[0045] The prepared vesicles were observed by electron microscope, as shown in figure 1 shown. It can be seen that the shape of the vesicles is regular, stable and uniform.
Embodiment 2
[0047] This example prepares fluorescent vesicles by the following method
[0048]2-oxooctanoic acid with a concentration of 200mM is dissolved in a mixed solvent of water and ethanol, wherein the mass fraction of ethanol is 50%, then adding phosphate buffered saline to adjust the pH of the solution to be 8, adding a photosensitizer, and mixing the solution uniformly Under light with a wavelength of 700nm, the reaction was carried out at a stirring rate of 300r / min, and fluorescent vesicles were obtained after 16 hours of reaction.
[0049] The prepared vesicles were observed by electron microscope, as shown in figure 2 shown. It can be seen that the shape of the vesicles is regular, stable and uniform. Fluorescent observation of the prepared fluorescent vesicles, such as image 3 , Figure 4 shown. As can be seen image 3 Medium fluorescent vesicles appear blue, Figure 4 Medium fluorescent vesicles appear green and have stable fluorescence properties.
Embodiment 3
[0051] This example prepares fluorescent vesicles by the following method
[0052] Dissolve nonanoic acid with a concentration of 50 mM in a mixed solvent of water and acetonitrile, wherein the mass fraction of acetonitrile is 50%, then add phosphate buffer to adjust the pH of the solution to be 5, and after the solution is uniformly mixed, under the light with a wavelength of 100 nm, The reaction was carried out at a stirring rate of 300 r / min, and fluorescent vesicles were obtained after 48 hours of reaction.
PUM
| Property | Measurement | Unit |
|---|---|---|
| wavelength | aaaaa | aaaaa |
| wavelength | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More