One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase

Abstract

The invention relates to a double-aqueous-phase one-step extraction method for bitter gourd saponin and polysaccharide with hypoglycemic activity. The double-aqueous-phase one-step extraction method comprises the steps: uniformly mixing dried bitter gourd powder with an isopropanol / (NH4)2SO4 double-aqueous-phase system, carrying out ultrasonic-microwave synergistic extraction, and then, separatinga crude bitter gourd saponin extracting solution serving as an upper phase and a crude bitter gourd polysaccharide extracting solution serving as a lower phase; carrying out rotary evaporation untilthe crude bitter gourd saponin extracting solution has no alcohol smells to prepare a bitter gourd saponin extracting solution, carrying out static adsorption and purification by using AB-8 type macroporous resin to obtain a refined bitter gourd saponin extracting solution, and carrying out freeze-drying to prepare a finished product of bitter gourd saponin; and carrying out absolute ethyl alcoholprecipitation, acetone washing and dialysis on the crude bitter gourd polysaccharide extracting solution to obtain a refined bitter gourd polysaccharide extracting solution, and carrying out freeze-drying to obtain a finished product of bitter gourd polysaccharide. The crude bitter gourd saponin extracting solution and the crude bitter gourd polysaccharide extracting solution are prepared at onestep by using an extraction method in which an ultrasonic-microwave assisted double aqueous phase system is adopted, the extraction process is simple, the cost is low, the extraction time can be effectively shortened, the extraction purity can be increased, the extraction steps can be reduced, and the effect on inhibiting alpha-glucosaccharase and alpha-amylase is relatively strong.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a two-phase aqueous phase one-step extraction method of bitter melon saponin and bitter melon polysaccharide and an in vitro hypoglycemic activity investigation. Background technique [0002] Bitter gourd, also known as bitter gourd, is a climbing plant of the genus Bitter gourd in the family Cucurbitaceae. It is widely planted in tropical and subtropical regions. It is a common vegetable in people’s lives. It has the effects of treating diabetes and so on, so it is known as "medicinal vegetable". Modern studies have shown that saponins and polysaccharides in bitter gourd are the main active components of its hypoglycemic effect. Therefore, the establishment of extraction technology for saponins and polysaccharides in bitter gourd is of great significance for guiding the deep processing of bitter gourd and the development of functional foods. At prese...

AI Technical Summary

Problems solved by technology

At present, most of the extraction processes for bitter melon saponins and bitter melon polysaccharides are separately extracted, and there is no report on the simultaneous extraction process of bitter melon saponins and polysaccharides, and it usually uses common organic solvent hot water bath or hot alcohol bath extraction, ultrasonic or microwave extraction, Macroporous adsorption resin extraction, enzyme extraction and other methods are mostly used alone or in combination, but there are still problems such as high energy consumption, long extraction time, low extraction purity, cumbersome operation, and organic solvent residues.

At present, there is no report on the one-step extraction process using ultrasonic-microwave-assisted two-phase system

In addition, there are many previous reports on the hypoglycemic effect of bitter melon, but most of them are researches on the hypoglycemic effect and mechanism of bitter melon saponins and polysaccharides in vivo through the establishment of type Ⅰ / Ⅱ diabetes animal models in rats, and bitter melon saponins and polysaccharides have no effect on blood sugar. There are few in vitro hypoglycemic studies on the inhibitory effect of α-glucosidase activity, especially the comparative study on the activity of Momordica saponin and polysaccharides in inhibiting α-glucosidase

In addition, the research on the activity of Momordica saponins and polysaccharides inhibiting α-amylase has not been reported yet.

Images