One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase
A technology of balsam pear saponins and balsam pear polysaccharides, applied in chemical instruments and methods, medical preparations containing active ingredients, glycoside steroids, etc., can solve the problems of cumbersome operation, organic solvent residues, and unseen problems, and achieve simple extraction process and low cost Low, avoid the effect of low biological activity
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[0055] Example 1
[0056] Preparation of bitter melon saponins and bitter melon polysaccharides
[0057] The process flow chart for the preparation of Momordica charantia saponin and Momordica charantia polysaccharide by one-step aqueous two-phase method is as follows figure 1 Shown. It mainly includes the following steps:
[0058] (1) Prepare isopropanol / (NH 4 ) 2 SO 4 Two-phase system: weigh out 53.655g (NH 4 ) 2 SO 4 In a 500mL reaction conical flask, add 185.71g 0.01mol / L PBS buffer (pH=7.0), shake to make (NH 4 ) 2 SO 4 Dissolve, then add 110.635g isopropanol, shake and mix well, then stand for layering;
[0059] (2) Preparation of bitter melon saponins and bitter melon polysaccharide crude extract: Weigh 5g dried bitter melon powder in 350g isopropanol / (NH 4 ) 2 SO 4 In the two-phase aqueous system, shake and mix well, and use CW-2000 ultrasonic-microwave cooperative extraction / reaction apparatus for extraction. The ultrasonic power was 50W, the microwave power was 800W, the ex...
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[0069] Example 2
[0070] Comparison and evaluation of the hypoglycemic activity of momordica charantia saponins and momordica charantia polysaccharides in vitro
[0071] In this example, the samples are acarbose, momordica charantia saponins, and momordica charantia polysaccharide. Momordica charantia saponins and Momordica charantia polysaccharides in vitro hypoglycemic activity test indicators are evaluated by their inhibition rate of α-glucosidase and α-amylase activity.
[0072] 1. Alpha-glucosidase system
[0073] Enzyme activity unit definition: at 37℃, pH=6.8, α-glucosidase hydrolyzes PNPG within 1 min to release 1μmol PNP; inhibitor activity unit: under the same conditions, it needs to reduce 1 enzyme activity unit The amount of inhibitor.
[0074] Reaction principle:
[0075] Reaction process: Using 50% dimethyl sulfoxide as solvent, prepare sample bitter melon saponin solution (concentration of 0.25, 0.50, 1.00, 2.00, 4.00 mg / mL) and bitter melon polysaccharide solution (c...
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