One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase

A technology of balsam pear saponins and balsam pear polysaccharides, applied in chemical instruments and methods, medical preparations containing active ingredients, glycoside steroids, etc., can solve the problems of cumbersome operation, organic solvent residues, and unseen problems, and achieve simple extraction process and low cost Low, avoid the effect of low biological activity

Active Publication Date: 2020-07-17
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the extraction processes for bitter melon saponins and bitter melon polysaccharides are separately extracted, and there is no report on the simultaneous extraction process of bitter melon saponins and polysaccharides, and it usually uses common organic solvent hot water bath or hot alcohol bath extraction, ultrasonic or microwave extraction, Macroporous adsorption resin extraction, enzyme extraction and other methods are

Method used

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  • One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase
  • One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase
  • One-step extraction method of Momordica saponin and polysaccharide with hypoglycemic activity in two-phase aqueous phase

Examples

Experimental program
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Example Embodiment

[0055] Example 1

[0056] Preparation of bitter melon saponins and bitter melon polysaccharides

[0057] The process flow chart for the preparation of Momordica charantia saponin and Momordica charantia polysaccharide by one-step aqueous two-phase method is as follows figure 1 Shown. It mainly includes the following steps:

[0058] (1) Prepare isopropanol / (NH 4 ) 2 SO 4 Two-phase system: weigh out 53.655g (NH 4 ) 2 SO 4 In a 500mL reaction conical flask, add 185.71g 0.01mol / L PBS buffer (pH=7.0), shake to make (NH 4 ) 2 SO 4 Dissolve, then add 110.635g isopropanol, shake and mix well, then stand for layering;

[0059] (2) Preparation of bitter melon saponins and bitter melon polysaccharide crude extract: Weigh 5g dried bitter melon powder in 350g isopropanol / (NH 4 ) 2 SO 4 In the two-phase aqueous system, shake and mix well, and use CW-2000 ultrasonic-microwave cooperative extraction / reaction apparatus for extraction. The ultrasonic power was 50W, the microwave power was 800W, the ex...

Example Embodiment

[0069] Example 2

[0070] Comparison and evaluation of the hypoglycemic activity of momordica charantia saponins and momordica charantia polysaccharides in vitro

[0071] In this example, the samples are acarbose, momordica charantia saponins, and momordica charantia polysaccharide. Momordica charantia saponins and Momordica charantia polysaccharides in vitro hypoglycemic activity test indicators are evaluated by their inhibition rate of α-glucosidase and α-amylase activity.

[0072] 1. Alpha-glucosidase system

[0073] Enzyme activity unit definition: at 37℃, pH=6.8, α-glucosidase hydrolyzes PNPG within 1 min to release 1μmol PNP; inhibitor activity unit: under the same conditions, it needs to reduce 1 enzyme activity unit The amount of inhibitor.

[0074] Reaction principle:

[0075] Reaction process: Using 50% dimethyl sulfoxide as solvent, prepare sample bitter melon saponin solution (concentration of 0.25, 0.50, 1.00, 2.00, 4.00 mg / mL) and bitter melon polysaccharide solution (c...

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PUM

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Abstract

The invention relates to a double-aqueous-phase one-step extraction method for bitter gourd saponin and polysaccharide with hypoglycemic activity. The double-aqueous-phase one-step extraction method comprises the steps: uniformly mixing dried bitter gourd powder with an isopropanol/(NH4)2SO4 double-aqueous-phase system, carrying out ultrasonic-microwave synergistic extraction, and then, separatinga crude bitter gourd saponin extracting solution serving as an upper phase and a crude bitter gourd polysaccharide extracting solution serving as a lower phase; carrying out rotary evaporation untilthe crude bitter gourd saponin extracting solution has no alcohol smells to prepare a bitter gourd saponin extracting solution, carrying out static adsorption and purification by using AB-8 type macroporous resin to obtain a refined bitter gourd saponin extracting solution, and carrying out freeze-drying to prepare a finished product of bitter gourd saponin; and carrying out absolute ethyl alcoholprecipitation, acetone washing and dialysis on the crude bitter gourd polysaccharide extracting solution to obtain a refined bitter gourd polysaccharide extracting solution, and carrying out freeze-drying to obtain a finished product of bitter gourd polysaccharide. The crude bitter gourd saponin extracting solution and the crude bitter gourd polysaccharide extracting solution are prepared at onestep by using an extraction method in which an ultrasonic-microwave assisted double aqueous phase system is adopted, the extraction process is simple, the cost is low, the extraction time can be effectively shortened, the extraction purity can be increased, the extraction steps can be reduced, and the effect on inhibiting alpha-glucosaccharase and alpha-amylase is relatively strong.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a two-phase aqueous phase one-step extraction method of bitter melon saponin and bitter melon polysaccharide and an in vitro hypoglycemic activity investigation. Background technique [0002] Bitter gourd, also known as bitter gourd, is a climbing plant of the genus Bitter gourd in the family Cucurbitaceae. It is widely planted in tropical and subtropical regions. It is a common vegetable in people’s lives. It has the effects of treating diabetes and so on, so it is known as "medicinal vegetable". Modern studies have shown that saponins and polysaccharides in bitter gourd are the main active components of its hypoglycemic effect. Therefore, the establishment of extraction technology for saponins and polysaccharides in bitter gourd is of great significance for guiding the deep processing of bitter gourd and the development of functional foods. At prese...

Claims

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Application Information

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IPC IPC(8): C07J17/00C08B37/00A61P3/10
CPCA61K36/42A61P3/10C07J17/005C08B37/0003C08B37/006
Inventor 刘杨王琦张杰良肖湘伦镜盛钟名其赵永杰
Owner SHANTOU UNIV
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