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Aggregation-induced emission material based nano microsphere, and preparation method and application of nano microsphere

A technology of aggregation-induced luminescence and nano-microspheres, which is applied in the direction of luminescent materials, microsphere preparation, and material analysis through optical means, which can solve problems that have not been seen before, achieve high stability, improve stability, and uniform fluorescence signals good sex effect

Pending Publication Date: 2018-09-28
ZHEJIANG ORIENT GENE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has not been used in fluorescent immunoassay techniques such as immunochromatography, microarray chips, and liquid phase chips.

Method used

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  • Aggregation-induced emission material based nano microsphere, and preparation method and application of nano microsphere
  • Aggregation-induced emission material based nano microsphere, and preparation method and application of nano microsphere
  • Aggregation-induced emission material based nano microsphere, and preparation method and application of nano microsphere

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Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0040] The nano-microspheres based on the aggregation-induced luminescent material prepared in this example, that is, the nano-microspheres are HPS / PSMA nano-microspheres, wherein the aggregation-induced luminescent material is 1,1,2,3,4,5-hexaphenylthiophene Sorrel (HPS), the polymer is styrene-maleic anhydride copolymer (PSMA), and the specific preparation steps are:

[0041] Step 1, 0.24g of 1,1,2,3,4,5-hexaphenylsilole (HPS) and 1g of styrene-maleic anhydride copolymer (PSMA) were dissolved in 48mL of toluene to form a dispersed phase ;

[0042] Step 2, the sodium dodecyl sulfate (SDS) of 8g is dissolved in the ultrapure water of 1.6L, forms continuous phase;

[0043] Step 3, using an SPG membrane with a pore size of 0.3 μm, under a nitrogen pressure of 200 kPa, under the shear force of the continuous phase, to prepare a uniform and stable oil-in-water emulsion;

[0044] Step 4, at room temperature, magnetically stir the obtained oil-in-water emulsion overnight, so that th...

preparation Embodiment 2

[0048] The nano-microspheres based on the aggregation-induced luminescent material prepared in this embodiment, that is, the nano-microspheres are TPE / PSMA nano-microspheres, wherein the aggregation-induced luminescent material is tetraphenylethylene (TPE), and the polymer is styrene-maleic anhydride Copolymer (PSMA), concrete preparation steps are:

[0049] Step 1, 0.5g of tetraphenylethylene (TPE) and 1g of styrene-maleic anhydride copolymer (PSMA) are dissolved in 48mL of trichlorotoluene to form a dispersed phase;

[0050] Step 2, the sodium dodecyl sulfate (SDS) of 8g is dissolved in the ultrapure water of 1.6L, forms continuous phase;

[0051] Step 3, using an SPG membrane with a pore size of 0.1 μm, under a nitrogen pressure of 600 kPa, under the shear force of the continuous phase, to prepare a uniform and stable oil-in-water emulsion;

[0052] Step 4, at room temperature, magnetically stir the obtained oil-in-water emulsion overnight, so that the solvent is volatiliz...

preparation Embodiment 3

[0056] The nano-microspheres based on the aggregation-induced luminescent material prepared in this embodiment, that is, the nano-microspheres are BDABFN / MOTAS nano-microspheres, wherein the aggregation-induced luminescent material is triphenylamine-fumaric acid nitrile compound (BDABFN), and the polymer is benzene Ethylene-acrylic acid copolymer (MOTAS), the specific preparation steps are:

[0057] Step 1, 0.1g of triphenylamine-fumaric acid nitrile compound (BDABFN) and 1g of styrene-acrylic acid copolymer (MOTAS) are dissolved in 48mL toluene to form a dispersed phase;

[0058] Step 2, the sodium dodecyl sulfate (SDS) of 4g and the polyvinyl alcohol of 4g are dissolved in the ultrapure water of 1.6L, form continuous phase;

[0059] Step 3, using an SPG membrane with a pore size of 0.3 μm, under a nitrogen pressure of 200 kPa, under the shear force of the continuous phase, to prepare a uniform and stable oil-in-water emulsion;

[0060] Step 4, at room temperature, magnetica...

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Abstract

The invention discloses an aggregation-induced emission material based nano microsphere and relates to the technical field of nano biological detection. The nano microsphere comprises the following ingredients: a polymer and an aggregation-induced emission material, wherein the aggregation-induced emission material is arranged in the polymer to form the nano microsphere; a particle size of the nano microsphere is 50-500nm; and a variation coefficient of the nano microsphere is less than 7%. The invention further provides the preparation method of the aggregation-induced emission material basednano microsphere. The aggregation-induced emission material based nano microsphere is controllable in emission wavelength and fluorescence intensity and easy in biomarking, and has the characteristics of high fluorescence intensity, high stability and good fluorescence signal uniformity, and a high stability, high repeatability and high sensitivity fluorescent immunodetection technology is obtained.

Description

technical field [0001] The invention relates to the technical field of nano-biological detection, in particular to a nano-microsphere based on an aggregation-induced luminescence material, a preparation method and an application thereof. Background technique [0002] Immunofluorescence technology is based on the specific recognition and combination between antigens and antibodies, and uses fluorescent materials to fluorescently label the detection target, and then detects the fluorescent signal generated by it at a certain excitation wavelength, and then performs qualitative and quantitative detection. This immunoassay method plays an important role in clinical medicine, modern life science, food science, ecology and other fields. Currently commonly used immunofluorescence techniques include: immunochromatography, microarray chip, liquid phase chip, etc. [0003] Based on these immunoassay techniques, currently commonly used fluorescent probes include: traditional organic f...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02B01J13/12G01N21/64
CPCB01J13/125G01N21/6428C09K11/025C09K11/06G01N2021/6432
Inventor 李万万武卫杰沈梦飞姜朝
Owner ZHEJIANG ORIENT GENE BIOTECH
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