Method for inducing resistance to broccoli gray mold by using exogenous salicylic acid
A technology of salicylic acid and broccoli, applied in the field of plants, can solve problems such as pollution, residue, health and environmental hazards, and achieve the effects of shortening time, reducing complicated work, and improving work efficiency
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Embodiment 1
[0017] Example 1 Salicylic acid (SA) is processed to the mensuration of the bacteriostatic activity of Botrytis cinerea of broccoli
[0018] 1. Preparation of 10mmol / L salicylic acid (SA) mother liquor: Take SA0.6906g in a beaker, add 400ml of sterile water, 2.5ml of absolute ethanol, heat in a water bath at 55°C and stir continuously with a glass rod to promote Dissolution of salicylic acid. After the salicylic acid is completely dissolved, adjust the pH to 6.8 with 1mol / L NaOH, and set the volume to 500ml for use.
[0019] 2. Preparation of potato dextrose agar (PDA) medium: take 200g of potatoes, wash them, remove the outer skin, cut them into small pieces, add water to boil for 20-30min, and poke them with a glass rod to form a homogenate. Filter with four layers of gauze, then add glucose and agar according to actual needs, continue heating and stirring to mix evenly, add water to 1000ml after cooling down, and pack in conical flasks with volumes of 12.5ml (a), 20ml ( ...
Embodiment 2
[0027] Example 2 Salicylic acid (SA) treatment to the induction effect determination of broccoli leaf gray mold resistance
[0028] 1. Preparation of different concentrations of salicylic acid (SA): Take the prepared 10mmol / L SA solution and dilute it into SA with concentrations of 0.002mmol / L, 0.02mmol / L, 0.2mmol / L, and 2mmol / L respectively. Solution, add 0.1% (V / V) Tween20.
[0029] 2. Salicylic acid (SA) treatment of broccoli leaves: put SA of each concentration in step 1 into the sprayer respectively, and spray the front and back of the leaves of the broccoli to be tested (to ensure that the growth status of each pot of broccoli is the same). Spray 3 pots for each concentration, and spray distilled water plus 0.1% (V / V) Tween20 as a control. Make a good mark and process for 3 consecutive days.
[0030] 3. Prepare broccoli gray mold microspore suspension: select a gray mold colony that has grown well for 10 days on the PDA medium, add a small amount of sterilized water on...
Embodiment 3
[0039] Example 3 Effect of Salicylic Acid Treatment on Broccoli Defense Enzyme Activity
[0040] 1. Salicylic acid (SA) treatment of broccoli leaves and sampling: put the prepared 2mmol / L SA into the sprayer, and repeatedly spray the front and back of broccoli leaves at the 4-leaf and 1-heart stage, every morning at 9 :00 Spray once at regular intervals for 3 consecutive days. The 4th day was recorded as the 1st day, and the leaves of 1, 3, 5, 7, 9, and 11 days were taken to measure the physiological indicators, and the physiological indicators measured by the untreated broccoli were used as the control, which was recorded as the 0th day of treatment.
[0041] 2. Enzyme solution extraction: Take 0.5 g of each treated broccoli leaf, add an appropriate amount of polyvinylpyrrolidone (PVP) and 5 ml of cold (4° C.) 0.5 mol / L pH 6.8 phosphate buffer. Add a small amount of liquid nitrogen to the mortar and cool it down, grind in an ice bath to break up the tissue, and centrifuge at...
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