Nano small peptide chelated iron for feed additive and preparation method of nano small peptide chelated iron

A feed additive and small peptide chelation technology, which is applied in the preparation method of peptides, animal feed, chemical instruments and methods, etc., can solve the problems of iron element absorption and pollution of the environment, and achieve the improvement of nutritional functionality and biological potency, Effects of cost reduction and immunity enhancement

Inactive Publication Date: 2018-10-19
河南广安生物科技股份有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned problem that directly adding inorganic iron in feed is not conducive to the absorption of iron by animals and pollutes the environment, the present invention provides a nano-small peptide chelated iron for feed additives and a preparation method thereof, the purpose of which is to: Nano-peptide chelated iron is used as a feed additive to enhance the nutritional functionality of iron in feed, increase the biological value of iron, and reduce environmental pollution

Method used

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  • Nano small peptide chelated iron for feed additive and preparation method of nano small peptide chelated iron
  • Nano small peptide chelated iron for feed additive and preparation method of nano small peptide chelated iron
  • Nano small peptide chelated iron for feed additive and preparation method of nano small peptide chelated iron

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preparation example Construction

[0029] A kind of preparation method of the nano small peptide chelated iron that is used for feed additive, comprises the steps:

[0030] [1] Dissolving soybean meal powder: dissolving soybean meal powder in a buffer solution with pH=2.2-8.0 to obtain a soybean meal powder solution;

[0031] [2] Preparation of dry peptide powder: heat-treat the soybean meal powder solution obtained in step [1], then add alkaline protease and flavor protease, heat for enzymolysis, then inactivate the enzyme activity in a boiling water bath, centrifugally filter to obtain the enzymolysis solution, and dry to obtain dry peptide powder. peptide powder;

[0032] [3] Preparation of nanometer small peptide chelated iron: dissolve the dry peptide powder obtained in step [2] to obtain a small peptide solution, add inorganic ferrous solution under heating and stirring conditions, and make the small peptide react with iron to form a chelate, After separation and drying, the nanometer small peptide chela...

Embodiment 1

[0045] A preparation method for feed additive nano small peptide chelated iron, comprising the steps of:

[0046] (1) Preparation of buffer solution: 1000 mL of buffer solution with pH 2.2-8.0 was prepared with 0.2M disodium hydrogen phosphate and 0.1M citric acid.

[0047] (2) Preparation of dry peptide powder: Dissolve 155g of soybean meal powder (particle size ≤0.6mm) in 1000ml of buffer solution, adjust the pH value to 4.3, acid-treat at 35-40°C for 10min; then heat-treat at 89-90°C 15min; then place the enzyme on a constant temperature shaker under the conditions that the mass ratio of alkaline protease to flavor protease is 1:1, the temperature is kept at 50-55°C, the pH is 7.0, and the enzyme concentration (M / S) is 10.5%. After hydrolysis for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, adjust the pH value to 4-4.5, then centrifuge at 3000 r / min for 10 minutes, filter to obtain the enzymatic hydrolysis solution, and spray dry to obtain dry pept...

Embodiment 2

[0053] A preparation method of feed additive nano small peptide chelated iron, the specific steps are as follows:

[0054] (1) Preparation of buffer solution: 0.2M disodium hydrogen phosphate and 0.1M citric acid were used to prepare a buffer solution with a pH of 2.2-8.0.

[0055] (2) Preparation of dry peptide powder: Dissolve soybean meal powder in the buffer solution of step (1) to make the concentration of soybean meal powder 165g / L, stir at 40-45°C for 15min; then heat-treat at 90-92°C for 20min; add Alkaline protease and flavor protease, at 55-60°C, pH=7.0, oscillating enzymolysis for 6 hours, after enzymolysis, inactivate the enzyme activity in a boiling water bath, adjust the pH value to 4.5, centrifuge, filter to obtain the enzymolysis solution, and dry , to obtain dry peptide powder;

[0056] (3) Preparation of small peptide solution: Dissolving dry peptide powder in water to make a saturated solution is small peptide solution;

[0057] (4) preparation of iron sol...

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Abstract

The invention relates to the technical field of chemical synthesis and in particular relates to nano small peptide chelated iron for a feed additive and a preparation method of the nano small peptidechelated iron. Aiming at the problems that inorganic iron mixed with feed directly is not beneficial to absorption of an iron element by pigs and the environment can be polluted, the invention has thetechnical scheme that the preparation method comprises the following steps: [1] dissolving soybean bran powder, namely dissolving soybean bran powder into a buffer solution of which the pH value is 2.2-8.0 so as to obtain a soybean bran powder solution; [2] preparing dried peptide powder, namely carrying out thermal treatment on the soybean bran powder solution obtained in the step [1], adding alkaline protease and flavourzyme, heating to carry out enzymolysis, carrying out boiling water bath enzyme deactivation, carrying out centrifugal filtration so as to obtain enzymatic hydrolysate, and drying so as to obtain dried peptide powder; [3] preparing nano small peptide chelated iron, namely dissolving the dried peptide powder obtained in the step [2] so as to obtain a small peptide solution, adding an inorganic ferrous solution under a heating stirring condition to enable small peptide and iron to react to form chelate, separating, and drying, thereby obtaining the nano small peptide chelated iron. The nano small peptide chelated iron is applicable to pig feed.

Description

technical field [0001] The invention relates to the technical field of chemical synthesis, in particular to a nanometer small peptide chelated iron used as a feed additive and a preparation method thereof. Background technique [0002] Iron is widely distributed in animals, almost all tissues contain iron. Among the parenchymal organs, the iron content in the spleen and kidney varied greatly, and in most animals, the iron content in the liver was particularly high. Iron not only plays an important role in the enzyme system in the body, but also is a component of hemoglobin and epidermal tissue. [0003] Most of the iron in animals exists in the form of protein complexes, and participates in material metabolism and energy metabolism in life activities in various forms. Iron is present in red blood cells as hemoglobin, in muscle as myoglobin, in serum as transferrin and ferritin, in the placenta as uterine ferritin, and as lactoferrin In milk, it exists in the liver and spl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/147A23K20/20C12P21/06C07K1/107
CPCA23K20/147A23K20/30C07K1/107C12P21/06
Inventor 张天勇王卫国高天增郭小云王全亮李志强万志友高翔胡启利梁晓晓陈青高鹏飞付充
Owner 河南广安生物科技股份有限公司
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