A molecularly imprinted electrochemical sensor for detecting patulin and its preparation method

A technology of molecular imprinting and patulin, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of high sensitivity and good selectivity, expensive equipment and complicated operation process, etc., and achieves low price and large ratio. Surface area, easy to prepare effect

Active Publication Date: 2019-11-15
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In recent years, the detection methods of patulin mainly include gas phase / liquid chromatography, gas phase / liquid chromatography tandem mass spectrometry, etc. These detection methods have high sensitivity and a large amount of sample detection, and are also very suitable for the separation and analysis of complex samples; but Its instruments and equipment are expensive and the operation process is cumbersome, especially not conducive to the realization of on-site on-line detection
Although electrochemical detection can achieve fast on-line detection, and is easy to operate and low in analysis cost, it is difficult to achieve high sensitivity and good selectivity.

Method used

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  • A molecularly imprinted electrochemical sensor for detecting patulin and its preparation method
  • A molecularly imprinted electrochemical sensor for detecting patulin and its preparation method
  • A molecularly imprinted electrochemical sensor for detecting patulin and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] The preparation of molecularly imprinted electrochemical sensors is mainly divided into the following three steps:

[0050] 1. The preparation of Th-PtNPs-NGEs (thionine-Pt nanoparticles-nitrogen-doped graphene) composite nanomaterials was synthesized by a two-step method, as follows:

[0051] The first step: 4.5mL of H 2 PtCl 6 ·6H 2 O aqueous solution (20mmol L -1 ) And 110μL hydrochloric acid aqueous solution (6mol L -1 ) Mix, disperse 50 mg of nitrogen-doped graphene in the mixed solution, and mix well with ultrasound. Then add 5.0mL of ascorbic acid aqueous solution (0.1mol L -1 ) As a reducing agent, ultrasonic 4h at room temperature for reduction reaction. After the reaction, the black reaction product was separated by centrifugation, washed three times with acetonitrile and distilled water, and dried at 50° C. to prepare Pt nanoparticles-nitrogen-doped graphene composite nanomaterials, and stored at room temperature for later use.

[0052] Step 2: Add 5 mg of the Pt n...

Embodiment 2

[0062] Patulin molecularly imprinted electrochemical sensor for determination of patulin in samples

[0063] All electrochemical experiments were carried out on the CHI 660 electrochemical analyzer (Shanghai Chenhua Instrument Company). The three-electrode system included: the molecularly imprinted electrochemical sensor prepared in Example 1 was used as the working electrode, and the saturated calomel electrode (SCE , Produced by Jiangsu Electrochemical Instrument Factory) as the reference electrode, and platinum wire electrode as the counter electrode.

[0064] All experiments were done at room temperature.

[0065] Differential pulse voltammetry (DPV) is used for testing, and the electrolyte is 0.1mol L -1 Phosphate buffer solution (PBS, pH 6.0), DPV scanning potential is from 0.2V to -0.6V. Finally, the patulin concentration in the solution to be tested is calculated and determined by the standard curve between the logarithm of the concentration of patulin in the solution (log C...

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Abstract

The invention provides a molecularly imprinted electrochemical sensor for detecting patulin. The sensor comprises a glassy carbon electrode, a thionine-Pt nanoparticle-nitrogen-doped graphene composite nanomaterial layer and a molecularly imprinted polymer layer. The molecularly imprinted electrochemical sensor can effectively amplify the signal, has high sensitivity and has good selectivity, a high recovery rate and good reproducibility. The molecularly imprinted electrochemical sensor is free of expensive large instruments, has a simple pretreatment process, ensures the accuracy of the results, realizes fast detection, greatly saves a cost and improves detection efficiency.

Description

Technical field [0001] The invention relates to the technical field of molecularly imprinted electrochemical sensors, in particular to a molecularly imprinted electrochemical sensor for detecting patulin and a preparation method thereof. Background technique [0002] Patulin (Patulin, PAT) also known as patulin, 4-hydroxy-4-hydrofuran(3,2C) pyran-2(6H)one (4-hydroxy-4H-furo[3,2C] pyran-2(6H)-one), the molecular formula is C 7 H 6 O 4 , Is a secondary metabolite of neurotoxic fungi, with potential teratogenicity, carcinogenicity and mutagenicity. Patulin is widely found in fruits and their products, and has a serious impact on human health and economy. International organizations and many countries have established the maximum limit of patulin. my country also stipulates that the limit of patulin in apples, hawthorn and their products cannot exceed 50μg kg -1 . Therefore, the detection and analysis method of patulin is very important and has become an international research hots...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 韩铮聂冬霞赵志辉蒋可秋黄晴雯郭文博孟佳佳
Owner SHANGHAI ACAD OF AGRI SCI
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