Heat-resistant low temperature exo-inulinase mutant MutQ23 delta 3

An exo-inulinase and mutant technology, which is applied in the field of genetic engineering, can solve the problems of poor heat resistance of low-temperature enzymes, and achieve the effect of improving thermal stability
CN108715840AActive Publication Date: 2018-10-30YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2018-10-30

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Abstract

The invention relates to the technical field of gene engineering and protein modification, in particular to a heat-resistant low temperature exo-inulinase mutant MutQ23 delta 3. Amino acid sequence ofthe mutant MutQ23 delta 3 is as shown in SEQ ID NO.1; the optimum pH of the MutQ23 delta 3 is 6.0, and the optimum temperature is 45 DEG C; the mutant MutQ23 delta 3 respectively contains 14%, 24%, 46% and 75% of enzyme activity at the temperature of 0 DEG C, 10 DEG C, 20 DEG C and 30 DEG C; the MutQ23 delta 3 still maintains 100% of enzyme activity after treatment for 60 minutes at the temperature of 50 DEG C; the enzyme can hydrolyze inulin to produce fructose. The heat-resistant low temperature exo-inulinase mutant MutQ23 delta 3 has the advantages of being applicable to industries such asfood, brewing and bioenergy.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and relates to protein transformation technology, in particular to a heat-resistant low-temperature exo-inulinase mutant MutQ23Δ3. Background technique

[0002] Inulin is a straight-chain fructan, which is connected by β-fructofuranose molecules through β-2,1 glycosidic bonds, and contains an α-glucopyranose group at its reducing end. Inulin is a plentiful renewable resource found in the roots and stems of plants such as Jerusalem artichoke, chicory, dahlia, burdock and yacon, which are prolific and can contain up to 22% fresh weight or 80% dry weight inulin. Inulinase has two modes of action: one is endo-inulinase, which hydrolyzes the β-2,1 glycosidic bonds inside the inulin polysaccharide chain to generate fructo-oligosaccharides; the other is exo-inulinase, which Hydrolyze β-2,1 glycosidic bonds one by one from the non-reducing end, and finally produce fructose and a small part o...

Claims

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