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Method for producing GO(graphene oxide)-PDA(polydiallyl phthalate)/PHBV(polyhydroxylbutyrate valerate) composite bone stent from dopamine modified oxidized graphene

A dopamine and bone scaffold technology, applied in the field of artificial bone scaffold materials, can solve the problems of poor polymer modification effect, inactivation of cells, and poor GO dispersion, and achieve the effects of improving biological properties, improving interaction, and promoting dispersion.

Active Publication Date: 2018-11-06
西安勃奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problems of poor hydrophilicity, low mechanical strength and poor cell compatibility of PHBV composite bone scaffold materials in the prior art, as well as poor dispersion of GO, the effect of polymer modification is poor, and the sharp edges of graphene oxide sheets can penetrate cell membranes and Destruction of the normal function of the cells and the loss of cell activity lead to limitations in its application in biomaterial modification. The purpose of the present invention is to provide a method for preparing GO-PDA / PHBV composite bone scaffold materials by modifying GO with dopamine. This method uses dopamine to modify GO to promote the dispersion of GO and prevent its agglomeration. At the same time, the sharp edges of graphene oxide sheets are modified to avoid damage to normal cells, which can better exert the biological activity of GO and obtain biological and mechanical properties. Better GO-PDA / PHBV Composite Bone Scaffold

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Disperse GO powder in deionized water with a mass concentration of 0.2mg / ml, and use a power of 500W for 50min to obtain an aqueous suspension of GO; first prepare a certain amount of 1mol / L PDA hydrochloride aqueous solution, take Mix 5 mL with 5 mL of the GO aqueous solution obtained above, stir at room temperature for 50 minutes, and raise the temperature. When the temperature of the reaction solution rises to 40°C, add a certain amount of 1 mol / L NaOH solution to adjust the pH of the reaction solution to about 8, and stir for 12 hours. , to finally obtain a uniform solution, then high-speed centrifugal washing, drying to obtain PDA functionalized GO nanocomposites (GO-PDA);

[0030] 2) Use an electronic balance to weigh 0.1 g of GO-PDA powder and 9.9 g of PHBV powder, and then add them to a beaker filled with 50 ml of absolute ethanol, and then the solution is mixed evenly by magnetic stirring and ultrasonic dispersion technology;

[0031] 3) After the mixed solu...

Embodiment 2

[0035] 1) Disperse GO powder in deionized water with a mass concentration of 0.3mg / ml, and use an ultrasonic wave with a power of 1000W for 40min to obtain an aqueous suspension system of GO; first prepare a certain amount of 1mol / L PDA hydrochloride aqueous solution, Take 5 mL, mix it with 5 mL of the GO aqueous solution obtained above, stir at room temperature for 50 minutes, and raise the temperature. When the temperature of the reaction solution rises to 60 ° C, add a certain amount of 1 mol / L NaOH solution to adjust the pH value of the reaction solution to about 9, and stir to react After 12 hours, a uniform solution was finally obtained, then washed by high-speed centrifugation, and dried to obtain PDA-functionalized GO nanocomposites (GO-PDA);

[0036] 2) Weigh 0.3 g of GO-PDA powder and 9.7 g of PHBV powder with an electronic balance, then add them into a beaker filled with 50 ml of absolute ethanol, and then mix the solution evenly by magnetic stirring and ultrasonic dis...

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Abstract

The invention discloses a method for producing a GO(graphene oxide)-PDA(polydiallyl phthalate) / PHBV(polyhydroxylbutyrate valerate) composite bone stent from dopamine modified oxidized graphene. The method comprises the following steps: depositing dopamine on the surface of oxidized graphene to obtain a GO-PDA powder; mixing the GO-PDA powder and a PHBV powder by using a liquid phase, performing solid-liquid separation to obtain a solid, drying and grinding the obtained solid to obtain a composite GO-PDA / PHBV powder; and performing selective laser sintering on the composite GO-PDA / PHBV powder to obtain the composite GO-PDA / PHBV bone stent. For the method, dopamine is utilized for modifying the oxidized graphene so as to promote the dispersion of the oxidized graphene, prevent the agglomeration while modifying sharp edges of oxidized graphene sheets to avoid damages on normal cells, so as to relatively well exert the biological activity of GO. Therefore, the composite GO-PDA / PHBV bone stent with relatively good biological performance and mechanical performance is obtained.

Description

technical field [0001] The invention relates to a composite bone support, in particular to a method for improving the performance of a polyhydroxybutyrate copolyester (PHBV) bone support by modifying graphene oxide with dopamine, and belongs to the technical field of artificial bone support materials. Background technique [0002] Polyhydroxybutyrate valeric acid copolyester (PHBV) is a biopolymer polyester with good biocompatibility and degradability. As an artificial bone scaffold implant material, it has attracted extensive attention from domestic and foreign scholars . However, PHBV itself has problems such as poor hydrophilicity, low mechanical strength, and poor cytocompatibility, which greatly limit its application in bone repair. Therefore, research on the modification of PHBV bone scaffold materials has become a hot spot. Adding a reinforcing phase with good compatibility with the matrix material is an effective method to improve the mechanical properties and hydro...

Claims

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Application Information

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IPC IPC(8): A61L27/44
CPCA61L27/443A61L2430/02C08L67/04
Inventor 帅词俊冯佩彭淑平王国勇叶恒云
Owner 西安勃奥生物科技有限公司
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