Side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof

A technology for luminescent detection and markers, which is applied in the field of immunological detection, can solve the problems of thermal damage detection background signal of biomolecules, and achieve good luminous effect

Active Publication Date: 2018-11-23
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the technical problems of thermal damage to biomolecules and high detection background signal of existing nanomaterials used in biological detection, and provides a lateral flow test based on near-infrared excitation and emission for serum marker luminescence detection. Paper strip and its preparation and method

Method used

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  • Side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof
  • Side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof
  • Side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof

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Experimental program
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Embodiment approach 1

[0043] Embodiment 1: The lateral flow test strip based on near-infrared excitation and emission detection of serum marker luminescence in this embodiment is composed of a polyvinyl chloride support backboard 1, a detection pad 2, a binding pad 3, a sample pad 4 and an absorption pad 5 composition;

[0044] Wherein the detection pad 2 is composed of a nitrocellulose membrane 2-1 and a detection line 2-2 and a control line 2-3 drawn parallel on the nitrocellulose membrane; the detection line 2-2 is a serum marker of 1-2 mg / mL The line drawn by the antibody has a line width of 1-1.2 mm, and the dropping volume is 1-1.2 μL / cm; the control line 2-3 is a line drawn with an IgG antibody solution of 1-2 mg / mL, and the line width is 1~1.2mm, drop volume is 1~1.2μL / cm;

[0045] Conjugation pad 3 is upconverting β-NaYF loaded with binding antibody 4 :Yb,Tm@NaYF 4 Nanocrystal probes and upconverting β-NaYF bound to mouse IgG 4 :Yb,Tm@NaYF 4 Nanocrystalline probe; where β-NaYF 4 :Yb,...

specific Embodiment approach 2

[0048] Specific embodiment two: The preparation method of the lateral flow test strip based on the near-infrared excitation and emission detection of the serum marker luminescent detection described in the specific embodiment one is carried out according to the following steps:

[0049] 1. Preparation of nuclear β-NaYF 4 :Yb,Tm, where β-NaYF 4 : Yb, the doping mole percentage of Yb element in Tm is 10%-99.8%, and the doping mole percentage of Tm element is 0.2%-10%:

[0050] a. According to β-NaYF 4 The stoichiometric ratio of Yb, Tm and the doping concentration of Yb and Tm take by weighing yttrium chloride, ytterbium chloride, thulium chloride, ammonium fluoride and sodium hydroxide;

[0051]b. First mix yttrium chloride, ytterbium chloride and thulium chloride in a container, then add oleic acid (OA) and octadecene (ODE), heat to 150-180°C and keep for 30-50 minutes to remove them moisture and oxygen, after cooling, a mixed solution is obtained;

[0052] c. Dissolve sod...

specific Embodiment approach 3

[0070] Specific embodiment three: what this embodiment is different from specific embodiment two is that the volume ratio of the total substance amount of yttrium chloride, ytterbium chloride and thulium chloride and oleic acid in step one (b) is 1mmol: (6 ~10) mL; the volume ratio of the total amount of yttrium chloride, ytterbium chloride and thulium chloride to octadecene is 1 mmol: (12-15) mL. Others are the same as in the second embodiment.

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Abstract

The invention provides a side flow test paper strip for serum marker luminescence detection based on near infrared excitation and emission and preparation and use methods thereof, and relates to a side flow test paper strip and preparation and use methods thereof. The technical problems of heat damage of the existing detection material on biomolecules and high background signals can be solved. Theside flow test paper trip consists of a polyvinyl chloride support back plate, a detection pad, a combination pad, a sample pad and an absorption pad, wherein the combination pad is loaded with an upper conversion beta-NaYF4:Yb,Tm@NaXF4 nanometer crystal probe combined with antibodies and an upper conversion beta-NaYF4:Yb,Tm@NaXF4 nanometer crystal probe combined with mouse IgG. The preparation method comprises the following steps of 1, preparing a core; 2, coating a shell; 3, assembling a luminous probe; 4, assembling the side flow test paper strip. In the use process, the laser is used forirradiating the detection line to detect the emission spectrum; the detection range is 0.05 to 50ng/mL; the detection limit is 0.02ng/mL; the side flow test paper strip can be used in medical care detection.

Description

technical field [0001] The invention relates to a lateral flow test strip for detecting serum markers and a preparation method thereof, belonging to the field of immunological detection. Background technique [0002] In recent years, with the continuous development of mobile medical care and home medical care, researchers are constantly seeking new types of luminescent materials as optical probes to obtain higher sensitivity and stability testing methods. Nowadays, people can detect various disease markers in serum, including myocardial infarction markers (CTNI and MYO); bacterial and viral infection markers (CRP, SAA and PCT); diabetes markers (glycosylated hemoglobin); Pro-infectious disease markers (HIV, syphilis, hepatitis B and C), etc. Among them, the detection method based on gold nanoparticles has developed rapidly, and has been rapidly applied in the fields of pregnancy detection and infection detection. However, the detection method based on gold nanoparticles ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63G01N33/68
CPCG01N21/63G01N33/6854G01N2333/47
Inventor 陈冠英冀天星王新栋
Owner HARBIN INST OF TECH
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