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An oxygen-enriched nano-biological enzyme electrode, sensor device, preparation method and application thereof

A sensor device, bio-enzyme technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that affect the accuracy of glucose detection, the inability to detect low-concentration glucose solution, toxicity, etc.

Active Publication Date: 2020-07-31
SCITECH CENTRY SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insufficient oxygen concentration limits the linear range of glucose detection;
[0004] 2) The oxygen content in the solution to be tested fluctuates, which limits the detection accuracy of the sensor;
[0005] 3) The detection of hydrogen peroxide usually needs to be carried out at a high potential of >0.6V. However, at this potential, most endogenous interfering substances (ascorbic acid, uric acid, etc.) and reducing drugs (acetaminophen, acetylsalicylic acid, etc.) acid, etc.) can also be oxidized on the electrode, thereby interfering with the glucose response signal and affecting the accuracy of glucose detection
These electronic mediators have certain toxicity to the human body, and are easy to fall off from the electrodes, with poor stability
[0008] 2) The electronic medium will compete with oxygen for electrons, resulting in oxygen interference, which will affect the detection accuracy of the glucose sensor, especially for low-concentration glucose solutions.
[0009] In the prior art, a three-phase oxygen-enriched electrode is used to solve the above problems. One end is in contact with the liquid to be measured, and the other end is in contact with an oxygen gas system. However, although this two-electrode or three-electrode system can be used for continuous measurement, the electrode structure is difficult. It is not easy to operate and carry, and cannot meet people's daily needs and mass production

Method used

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  • An oxygen-enriched nano-biological enzyme electrode, sensor device, preparation method and application thereof
  • An oxygen-enriched nano-biological enzyme electrode, sensor device, preparation method and application thereof
  • An oxygen-enriched nano-biological enzyme electrode, sensor device, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0106] Hollow mesoporous silica spheres were prepared by the following method:

[0107] (1) 25ml aqueous solution including 100nm polystyrene microspheres 0.2wt%, cetyltrimethylammonium bromide 0.2wt%, sodium hydroxide 0.056wt% and 1,2-bis(triethoxysilyl) Ethane 0.768wt%, stirred and reacted at 80°C for 2 hours, then centrifuged and washed 3 times with water and ethanol to obtain a sample.

[0108] (2) Drop the polystyrene@mesoporous silica balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcine for 6 hours with oxygen at 550°C.

[0109] (3) Scrape the hollow mesoporous silica sphere prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of hollow mesoporous silica sphere ethanol or aqueous solution.

[0110] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven in ...

Embodiment 3

[0119] Hollow mesoporous silica spheres were prepared by the following method:

[0120] (1) 25ml aqueous solution including 100nm polystyrene microspheres 0.2wt%, cetyltrimethylammonium bromide 0.2wt%, sodium hydroxide 0.056wt% and 1,2-bis(triethoxysilyl) Ethane 0.576wt%, stirred and reacted at 80°C for 2 hours, then centrifuged with water and ethanol for 3 times to obtain a sample.

[0121] (2) Drop the polystyrene@mesoporous silica balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcine for 6 hours with oxygen at 550°C.

[0122] (3) Scrape the hollow mesoporous silica sphere prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of hollow mesoporous silica sphere ethanol or aqueous solution.

[0123] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven in an oven...

Embodiment 4

[0132] Prepare hollow alumina spheres by the following method:

[0133] (1) Dissolve 0.6303g of ammonium formate in 50ml of deionized water, and adjust the pH to 4.4 with formic acid. 3 ml (2.5 wt %) of an aqueous solution of 100 nm polystyrene microspheres and 0.351 g of aluminum sulfate sonicated for 15 minutes were added. After reacting at 70°C for two hours, centrifuge and wash with water and ethanol.

[0134] (2) Drop the polystyrene microspheres@alumina balls washed in (1) on the cleaned glass slide, put them into a tube furnace, and calcinate at 550°C for 2 hours with oxygen.

[0135] (3) Scrape the hollow alumina ball prepared in (2) from the glass slide with a clean blade, add a certain amount of ethanol or deionized water, and make a certain amount of ethanol or aqueous solution of the hollow alumina ball.

[0136] The above slide cleaning method is to use ethanol: acetone: water as a 1:1:1 organic solvent as a cleaning agent, ultrasonic for 15 minutes, and an oven...

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Abstract

An oxygen-enriched nano bio-enzyme electrode, a sensor device, a preparation method, and application. According to the oxygen-enriched nano bio-enzyme electrode, an electrode substrate is modified with a hollow nano material having an oxygen enriching function, hydrogen peroxide catalyst particles, and an oxidase corresponding to an object under test. The oxygen-enriched nano bio-enzyme electrode and device have a wide detection linear range, high sensitivity, and good interference resistance performance, and can be mass produced. These advantages mainly derive from the hollow material having an oxygen storage function.

Description

technical field [0001] The invention relates to the technical field of electrode material preparation and electrochemical detection, in particular to an oxygen-enriched nano-biological enzyme electrode material, a sensor device, a preparation method and application thereof. Background technique [0002] Glucose is a worldwide public health problem and has become a dangerous disease second only to cardiovascular disease, namely cancer. Today, there are as many as 300 million people with diabetes in the world, among which there are as many as 100 million people with severe diabetes who need real-time control of blood sugar concentration. Therefore, it is of great significance to realize accurate and rapid detection of blood sugar of diabetic patients in daily life. Since 1962, when Leland Clark first proposed a theoretical model for measuring glucose with an enzyme electrode, the research on glucose sensors has never stopped. Nowadays, amperometric glucose sensors are mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 封心建唐鹤鸣
Owner SCITECH CENTRY SUZHOU BIOTECH CO LTD