Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Detection method of lincomycin fermenting liquid

A lincomycin and detection method technology, applied in the detection field of lincomycin fermentation broth, can solve problems such as insufficient accuracy and reproducibility, achieve reagent consumption and labor saving, good peak shape, and good separation Effect

Active Publication Date: 2018-11-23
JIANGXI GUOYAO PHARMA LLC
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of lincomycin fermentation liquid unit is most commonly used by biological detection or optical rotation detection, but the accuracy and reproducibility are not good enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of lincomycin fermenting liquid
  • Detection method of lincomycin fermenting liquid
  • Detection method of lincomycin fermenting liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The fermentation broth of lincomycin was prepared, and the strain used was StreptomyceslincolnensisCPCC 280065 (source: China Medical Microorganism Collection and Management Center).

[0030] Culture conditions:

[0031] The strains were cultured in a slant medium at 30°C for 6 days. The composition of the slant medium was (parts by mass): starch 2.0, cold soybean meal 0.5, K 2 HPO 4 0.05, NaCl 0.05, MgSO 4 ·7H 2 O 0.05, agar 2.5, pH 7.0; after that, the digging pieces of bacteria grown on the slope are inoculated into the seed culture medium for 2 days, the culture temperature is 30°C, and the composition of the seed culture medium is (mass parts): starch 2.0, glucose 2.5, soybean meal 2.0, corn syrup 2.5, ammonium sulfate 0.2, sodium chloride 0.07, calcium carbonate 0.8, pH 7.0;

[0032] Lincomycin fermentation conditions:

[0033] The cultivated seeds were inoculated into the fermentation medium at an inoculum of 10% and cultivated for 7 days at a temperature of 30°C. The c...

Embodiment 2

[0035] Methodological experiment:

[0036] 1. Repeatability test

[0037] Test operation: Take the same batch of lincomycin fermentation broth, process it according to the above method, inject 20.0μL, inject two injections of each sample, record the chromatogram and chromatographic data, and calculate the lincomycin content according to the external standard method , RSD% shall not be greater than 2%, the results are shown in Table 1.

[0038] Table 1 Repetitive test results of lincomycin fermentation broth

[0039]

[0040] 2. Intermediate precision test

[0041] Test operation: Choose three batches of lincomycin fermentation broth, and different analysts will determine the content of lincomycin in the same laboratory for three days, and then compare the determination results to determine whether the intermediate precision meets the requirements, accept Standard: The relative standard deviation shall not be greater than 2%. The results are shown in Table 2-4.

[0042] Table 2 Intermed...

Embodiment 3

[0066] A method for detecting lincomycin fermentation broth includes the following steps:

[0067] S1: Preparation of the test solution: Take the lincomycin fermentation broth, centrifuge at 4000r / min for 5 minutes to separate and remove the mycelium and solid impurities to obtain the supernatant, take the supernatant, dilute 1 times the volume with the mobile phase, and use The polyethersulfone membrane with a pore size of 0.22μm is filtered as the test solution;

[0068] Among them, the polyethersulfone membrane is pre-activated before filtration: 95% ethanol is filtered with a polyethersulfone membrane for 15 minutes;

[0069] S2: Preparation of reference substance solution: accurately weigh 60mg of lincomycin reference substance into a 50ml volumetric flask, dissolve and dilute to the mark with mobile phase, and shake well to obtain reference substance solution;

[0070] S3: Detection method: accurately draw 15 μL of the reference solution and the test solution respectively, and i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention discloses a detection method of a lincomycin fermenting liquid. The detection method has the advantages that the pretreatment method of the lincomycin fermenting liquid is optimized; a fermenting supernatant is diluted by a flow phase containing a small amount of dodecyl dimethyl betaine, and an activated polyether sulfone film is used for filtering, so as to greatly reduce the amount of impurities in the lincomycin fermenting liquid; a HPLC (high performance liquid chromatography) detection method of the lincomycin fermenting liquid is optimized, the pH (potential of hydrogen) value of a phosphate buffer solution is adjusted by ammonia water, and then the phosphate buffer solution is mixed with acetonitrile and methanol to prepare the flow phase, so that the peak emitting time is shortened, the peak shape is good, the trailing is avoided, the peak is easy to separate from the nearby impurity peak, and the theoretical plate numbers of column pressure and main peak are notobviously changed after a chromatographic column is used for continuously analyzing a plurality of samples.

Description

Technical field [0001] The invention relates to a detection method of lincomycin fermentation broth, belonging to the field of drug detection. Background technique [0002] Lincomycin belongs to the lincomycin class of antibiotics. It is effective against most Gram-positive bacteria and some anaerobic Gram-negative bacteria. The effect on positive bacteria is similar to erythromycin. It is mainly used for abdominal cavity and gynecological infections caused by anaerobic bacteria. It is also commonly used for various serious infections caused by sensitive positive bacteria. Because it is concentrated in bone tissue, it is the first choice for staphylococcus aureus osteomyelitis. Its clinical application More extensive. At present, the most commonly used detection of lincomycin fermentation broth unit is biological detection or polarimetric detection, but the accuracy and reproducibility are not good enough. [0003] Compared with biological detection or polarimetric detection, hig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 詹业奇万义斌葛友群左飞鸿杨明于莲欣李进进罗进艾嘉皓
Owner JIANGXI GUOYAO PHARMA LLC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com