Anthocyanin-enriched aronia melanocarpa fruit vinegar drink and preparation method thereof
A fruit vinegar drink containing anthocyanin technology, applied in the field of food processing, can solve the problems of bitter taste, reduce the type and amount of additives, and not easy to drink, and achieve the effects of easy acceptance, avoiding yeast inhibition, and avoiding degradation
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[0064] Preparation of acetic acid bacteria fermentation medium:
[0065] Yeast extract 10g / L, fishmeal peptone 20g / L, glucose 20g / L, dissolved in 1L water in proportion, autoclaved at 115°C for 15min and cooled to room temperature, adding 3mL absolute ethanol to every 100mL medium.
[0066] The detection method that the present invention relates to is as follows:
[0067] Alcohol testing method: refer to the alcohol meter method in GB 5009.225-2016. Add 50mL sample liquid, 50mL deionized water and 3 glass beads (to prevent bumping) into the distillation flask; open the condensed water; heat, and use a 50ml measuring cylinder to receive the distillate; when the volume of the distillate reaches about 45ml, remove the measuring cylinder , add deionized water until the concave surface is flush with the 50ml scale; mix well with an alcohol meter, read the reading of the alcohol meter, then insert the thermometer, and read the reading of the thermometer; convert it to Alcohol cont...
Embodiment 1
[0087] Embodiment 1: Alcoholic fermentation
[0088] (1) Squeeze aronia to produce juice, and separate the squeezed juice from pomace and peel to obtain aronia juice, aronia pomace, and peel;
[0089] (2) Measure the initial sugar concentration of aronia juice, add sugar to 220g / L at one time, and stir evenly;
[0090] (3) Add 160 mg / L of potassium metabisulfite and 100 mg / L of pectinase, and stir evenly;
[0091] (4) Take the Saccharomyces cerevisiae BYC3 bacterial solution with a bacterial concentration of 2‰ and activate it in ten times the volume of aronia berry juice with an initial sugar concentration of 30g / L, 40g / L, 50g / L, 60g / L, and 70g / L , activated at 28°C for 18 hours, 50g / L was the implementation group, and the rest were the comparison group;
[0092] (5) Put the activated yeast seed liquid into aronia berry juice, and ferment at a controlled temperature of 22°C. After 7 days of fermentation, when the surface of the wine body is calm and there are few bubbles, t...
Embodiment 2
[0097] Embodiment 2: acetic fermentation
[0098] (1) prepare the acetic acid bacteria fermentation medium, put the medium into the fermenter;
[0099] (2) Take Acetobacter pasteurii CYD159 and inoculate it into YPD medium with 2% inoculum amount, 30°C, 200r / min, shake the constant temperature for 24h, and then use it as the primary seed liquid; draw 1mL of the primary seed liquid and insert it into the YPD liquid In the culture medium, under the conditions of 30°C and 200r / min, shake the constant temperature culture for 10h, then put it into the fermenter, which is the secondary seed liquid;
[0100] (3) Add YPD medium in advance in the fermenter, sterilize at 121°C and 0.1MPa for 15min, add the aronia fruit wine obtained in Example 1 after cooling (where the fruit wine is obtained from the implementation group in Example 1), control The alcohol content is not more than 3%, inoculated into 10% secondary seed liquid, and the conditions are strictly controlled during the ferme...
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