Multi-drug-carrying targeted nanoparticle and preparation method and application thereof

A nanoparticle, multi-drug loading technology, applied in the field of biomedicine, to achieve the effect of prevention and treatment of acute liver failure

Inactive Publication Date: 2018-12-21
CHONGQING UNIV OF EDUCATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lack of chemoprevention and adjuvant therapy remain major barriers to successful sepsis treatment

Method used

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  • Multi-drug-carrying targeted nanoparticle and preparation method and application thereof
  • Multi-drug-carrying targeted nanoparticle and preparation method and application thereof
  • Multi-drug-carrying targeted nanoparticle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of multidrug-loaded targeting nanoparticles

[0063] 1. Preparation of gold-coated iron oxide core-shell nanoparticles (GNPs)

[0064] 0.1M FeSO 4 ·7H 2 O aqueous solution and 0.2M FeCl 3 · 6HO was placed in 90ml solution containing 0.1M HCl. Then 80 ml of 2M sodium hydroxide were carefully added dropwise and the reaction was allowed to distribute well for 70 minutes with continuous stirring and nitrogen protection. The resulting black suspension was collected using a high power neodymium alloy magnet (2500G) and washed 3-4 times with nitrogen-treated double-steamed water. A 0.1 g / mL citrate working solution was subjected to a stable iron oxide nanoparticle suspension at pH 6.8, and the mixture was stirred continuously and heated to 80 °C for 2 h. The solution was then kept to cool down to room temperature to obtain citric acid-stabilized Fe3O4 nanoparticles. 60 mg of citric acid-coated iron oxide nanoparticles were dispersed in 15 mL of doub...

Embodiment 2

[0067] Example 2 Characterization of multidrug-loaded targeting nanoparticles

[0068] Electron micrographs of FN nanoparticles were examined using TEM (Tecnai G2 20200 kV TEM (Fei, Electron Optics)). A small drop of the sample solution in double-distilled water was dried on a carbon-coated copper grid for TEM analysis. In addition, the average size of the FN nanoparticles we obtained above was checked using dynamic light scattering (DLS) with a LB-550DLS particle size analyzer (Horiba Scientific, Edison, NJ). Magnetic properties of the nanoparticles were examined at room temperature (25°C) using a Lake Shore 735VSM.

[0069] TEM analysis of the nanoparticles showed that the nanoparticles had a spherical morphology ( image 3). Dynamic light scattering revealed that these nanoparticles had an average hydrodynamic diameter of 15 nm ( Figure 4 ). The gold-coated iron oxide nanoparticles appear darker in the studied region due to the high electron density of gold. In addit...

Embodiment 3

[0070] Example 3 Multidrug-loaded targeted nanoparticles inhibit LM-induced metabolic abnormalities and systemic inflammation in mice

[0071] To identify Listeria monocytogenes (LM)-induced lethal sepsis as the cause of metabolic system disturbance and systemic inflammatory response, and then evaluate the effect of FN nanoparticle intervention on improving the abnormal physiological state. In our study, LM administration significantly induced metabolic disorders compared with control mice. All mice in the model group died within 42 h after injection of LM. The survival rates of mice in the FN nanoparticle intervention group were 0% (25 / 25; 54h after injection; 20mg / kg FN), 36% (9 / 25; 40mg / kg FN) and 60% (15 / 25; 80mg / kg FN). kg FN)( Figure 8 A). Additionally, LM infection altered respiratory rate, blood pressure and body temperature in mice. Figure 8 B-8D shows increased respiratory rate, hypothermia and dyskinesia in LM-infected mice. However, FN nanoparticle treatment...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a multi-drug-carrying targeted nanoparticle and a preparation method and application thereof. The multi-drug-carrying targeted nanoparticle is prepared by loading a polyphenol compound, iRhom2 siRNA and TNF-Alpha inhibitor on a gold-coated iron oxide core-shell nanoparticle (GNPs), and the molar ratioof the gold-coated iron oxide core-shell nanoparticle, the polyphenol compound, iRhom2 siRNA and the TNF-Alpha inhibitor is 1:(1 to 3):(1 to 3):(1 to 3). The multi-drug-carrying targeted nanoparticledisclosed by the invention increases the bioactivity and bioavailability of the polyphenol compound, and can effectively prevent and treat related diseases as the result of septicemia caused by listeria monocytogenes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a multidrug-loaded targeting nanoparticle and a preparation method and application thereof. Background technique [0002] The innate immune system is the host's key and first line of defense against foreign pathogens by eliciting highly complex adaptive immune responses. When an infectious organism is identified, the host innate immune system recognizes the invading microorganism and initiates a defense response and inflammatory processes. Dysregulated inflammatory responses are an important component of innate immunity and contribute to the occurrence of many diseases, including persistent tissue damage, balance disturbance, physiological abnormalities and even death. Sepsis is a serious inflammatory disease caused by bacterial infection. Immunosuppression and uncontrolled inflammatory responses have been demonstrated to be one of the major pathophysiological m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/352A61K31/7105A61K31/454A61K9/51A61K47/02A61P31/04A61P1/16
CPCA61K9/5115A61K31/352A61K31/454A61K31/7048A61K31/7105A61P1/16A61P31/04A61K2300/00
Inventor 徐敏轩葛晨旭谭君娄德帅李强胡林峰
Owner CHONGQING UNIV OF EDUCATION
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