Paenibacillus polymyxa with saline-alkali resistant effect and application thereof

A polymyxa-like, salt-alkali-resistant technology, applied in the field of agricultural biology, to achieve strong salt-alkali tolerance, promote plant growth, and reduce continuous cropping obstacles

Active Publication Date: 2018-12-21
SHANDONG ZOETICLAND BIOLOGICAL TECH +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to reports, many microorganisms have the ability to degrade phenolic acids, including more than 20 species such as Phanerochaete chrysosporium, Serratia marcescens, Azospirillum adipogenicum, and Bacillus amyl

Method used

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  • Paenibacillus polymyxa with saline-alkali resistant effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening of Paenibacillus polymyxa PAPM17

[0026] Paenibacillus polymyxa PAPM17 was isolated from cotton rhizosphere soil in saline-alkali land. The soil was sampled in Yongan Town, Kenli District, Dongying City, Shandong Province, which was a saline-alkali soil where cotton had been planted continuously for more than 5 years. The specific separation method is as follows: weigh 5 g of the mixed soil sample, put it into 100 mL of enrichment medium, and culture it with shaking at 37°C and 150 rpm for 5 days. Take 1 mL of the enriched culture for 10 -1 ~10 -5 Serial dilution, then take 10 -3 、10 -4 、10 -5 The three dilutions were spread on the plate containing the selection medium, and cultured at 37°C for 5 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured at 37°C for 5 days. Pick a single colony and transfer it to the slant of the preservation medium test tube, culture it at 37°C for 2 days, and after...

Embodiment 2

[0033] Example 2: Identification of Paenibacillus polymyxa PAPM17

[0034] (1) Morphological and physiological and biochemical characteristics

[0035] The morphological characteristics of the PAPM17 strain are: cultured at 37°C for 36 hours on a nutrient agar medium, the colony is milky white, round, raised, shiny, smooth, moist, sticky, with neat edges, and difficult to pick; Rod-shaped, Gram-positive, spore-forming, oval-shaped spores, not enlarged, and without paraspore crystals. The formula of the nutrient agar medium is 10 g of peptone, 3 g of beef extract, 5 g of NaCl, 20 g of agar, 1000 ml of water, and pH 7.5.

[0036] The physiological and biochemical characteristics of the PAPM17 strain are: negative indole test, positive nitrate reduction test, positive v-p test, positive casein hydrolysis test, positive starch hydrolysis test, positive 5% NaCl test, positive L-arabinose test, D - Positive glucose test, positive D-xylose test, negative citrate utilization test, p...

Embodiment 3

[0040] Example 3 Degradation test of Paenibacillus polymyxa PAPM17 to gallic acid, p-hydroxybenzoic acid and ferulic acid

[0041] Prepare a medium containing p-hydroxybenzoic acid, its formula is: p-hydroxybenzoic acid 1000mg, peptone 5g, ammonium sulfate 0.5g, magnesium sulfate 0.1g, potassium chloride 0.2g, sodium chloride 0.5g, water 1000ml, pH 7.5 .

[0042] A culture medium containing gallic acid was prepared, and its formula was: 1000 mg of gallic acid, 5 g of peptone, 0.5 g of ammonium sulfate, 0.1 g of magnesium sulfate, 0.2 g of potassium chloride, 0.5 g of sodium chloride, 1000 ml of water, and pH 7.5.

[0043] A culture medium containing ferulic acid was prepared, and its formula was: 1000 mg of ferulic acid, 5 g of peptone, 0.5 g of ammonium sulfate, 0.1 g of magnesium sulfate, 0.2 g of potassium chloride, 0.5 g of sodium chloride, 1000 ml of water, and pH 7.5.

[0044] Paenibacillus polymyxa PAPM17 was transferred into the above-mentioned three kinds of medium, ...

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Abstract

The invention discloses paenibacillus polymyxa with saline-alkali resistant effect and application thereof. The paenibacillus polymyxa is paenibacillus polymyxa PAPM17 isolated from the rhizosphere soil of cotton in a saline-alkali land, and has been collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on July 17th, 2018; the microorganism collection number is CGMCC No.16118. The paenibacillus polymyxa has the advantages that the paenibacillus polymyxa can survive in the soil of the saline-alkali land, the better degrading effect is realized onthe self-poisonous matters of plants, such as gallic acid, p-hydroxybenzoic acid and ferulic acid, the continuous cropping obstacles are reduced, and the functions of accelerating growth and increasing output are realized on the cultivated cotton in the saline-alkali land; the paenibacillus polymyxa is inoculated into a fermenting culture medium using soybean meal powder, corn pulp and molasses as the main nutrient sources to ferment, added with an additive, and spray-dried, so as to obtain the live bacterial preparation; the fermenting cycle is short, the loss of live bacteria in the spray-drying process is low, and the stability is high in the preservation process.

Description

technical field [0001] The invention relates to a saline-alkali-tolerant Paenibacillus polymyxa and its application, belonging to the field of agricultural biotechnology. Background technique [0002] Saline-alkali land is an important land resource in my country and has great potential for development. According to the Nanjing Soil Research Institute of the Academy of Agricultural Sciences, the total area of ​​saline-alkali land and saline-alkali barrier cultivated land in my country exceeds 500 million mu, of which there are as many as 200 million mu with agricultural utilization potential, accounting for about 10% of China's cultivated land area, mainly distributed in Northwest my country, Northeast and eastern coastal areas. Rational development and utilization of saline-alkali land is of great significance to ensure food security in my country, promote sustainable agricultural development, improve ecological environment and promote coordinated development of regional ec...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/04C05F11/08A01N63/00A01P21/00C12R1/01C05G3/80
CPCA01N63/00C05F11/08C12N1/20C05G3/80C12N1/205C12R2001/01Y02A40/10
Inventor 孙中涛辛寒晓姚强孙国科陈君君
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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