Preparation method for chicken interferon-alpha

A technology of chicken interferon and alpha gene, which is applied in the field of preparation of chicken interferon alpha, can solve problems such as lack of biological activity, and achieve the effects of simple expression scheme, favorable absorption and high yield
CN109134641AInactive Publication Date: 2019-01-04HANGZHOU JUNFENG BIOENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU JUNFENG BIOENG
Publication Date
2019-01-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of animal biological products, in particular to a preparation method for chicken interferon-alpha. The preparation method for the chicken interferon-alpha comprisesthe steps that an optimized gene sequence is utilized, the expression quantity of the optimized gene sequence is higher than that of an existing gene, a chicken interferon-alpha gene is obtained, an expression carrier is constructed, escherichia coli engineering bacteria are converted, careening, culturing and inducible expression are conducted, an expression thallus is subjected to collection andbacterium breaking, an inclusion body is subjected to washing, splitting and renaturation, and the chromatographic technology is adopted for purification to obtain the chicken interferon-alpha. The preparation method has significant value on production of the chicken interferon-alpha.
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Description

technical field

[0001] The invention relates to the field of veterinary biological products, in particular to a preparation method of chicken interferon alpha. Background technique

[0002] Infectious diseases are important diseases that endanger the healthy development of my country's poultry industry, especially viral diseases, such as vesicular stomatitis virus, Rous sarcoma virus, Newcastle disease virus, infectious bursal disease virus, infectious bronchitis Virus, Marek virus, bird flu virus, etc. Once these infectious diseases break out, they will spread very quickly and cause serious harm. Chicken infectious bronchitis is an acute highly contagious infectious disease caused by a virus. It occurs frequently in winter and spring every year. Due to the sudden onset and rapid death of the disease, it often causes large economic losses to farmers. Avian influenza is another infectious disease with high incidence. It is infectious to many kinds of animals. Highly pathogeni...

Claims

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