Herbivorous fish protein feed made from sugarcane shoots and leaves and manufacturing method thereof
A production method and herbivorous technology, applied in the field of herbivorous fish sugarcane tip leaf protein feed and its production, can solve the problems of large volume, inconvenient transportation, waste of resources, high water content of sugarcane tip leaves, etc. Feed protein content, improve fermentation efficiency, and broaden the effect of application fields
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Embodiment 1
[0027] 1. Screening, purification, preservation, activation and expansion of strains
[0028] Aspergillus aculeatus and white rot fungus were isolated and screened from bagasse that had been piled up for 3 years. After purification and cultivation, they were placed in a -80°C refrigerator.
[0029] Get the following strains in parts by weight: 0.8 part of Aspergillus aculeatus, 0.8 part of white rot fungus, 0.8 part of Bacillus subtilis, 1 part of Candida utilis and 1 part of Saccharomyces cerevisiae, after activation, according to the percentage by weight, Use 20% of raw molasses, 0.15% of urea, 0.13% of concentrated phosphoric acid and add 32°C of added temperature to evaporate condensed water to 100% as a culture medium, insert bacteria, expand and cultivate in a culture tank for 2 days, and obtain composite bacteria for use.
[0030] 2. Sugarcane tip leaf pretreatment
[0031] Cut 600 parts of sugarcane tip leaves into 4-8cm and rub for 8 minutes.
[0032] Spray evaporat...
Embodiment 2
[0043] 1. Screening, purification, preservation, activation and expansion of strains
[0044] Aspergillus aculeatus and white rot fungus were isolated and screened from the bagasse that had been stacked for 2 years. After purification and cultivation, they were placed in a -80°C refrigerator.
[0045] Get the following bacterial species in parts by weight: 1 part of Aspergillus aculeatus, 1 part of white rot fungus, 1 part of Bacillus subtilis, 1.2 parts of Candida utilis and 1.2 parts of brewer's yeast. After activation, according to the weight percentage, Using 25% raw molasses, 0.18% urea, and 0.15% concentrated phosphoric acid as the medium, and adding condensed water at a temperature of 25°C to 100%, inserting the strains, expanding the culture in the culture tank for 3 days, and obtaining the composite bacteria for use.
[0046] 2. Sugarcane tip leaf pretreatment
[0047] Cut 800 sugarcane tip leaves into 4-8cm and rub for 10min.
[0048] On the fresh sugarcane tip lea...
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