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Device and method for online heating separation of microbial oil, and microbial oil

A technology of microbial oil and oil-producing microorganisms, applied in the direction of fat production, fat oil/fat refining, fat oil/fat production, etc., can solve the problems of aggravated oil oxidation, long time, poor oil quality, etc., to slow down the speed and degree, heat The effect of shortening the time and reducing the production cost

Active Publication Date: 2019-01-11
梁云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The addition of electrolytes will cause the generation and pollution of harmful substances such as 3-chloropropanol, which will aggravate the generation of the pollutants; the method of increasing the temperature of the feed liquid is usually heated by fermentation tanks or enzymatic hydrolysis tanks in the industry, and the inner coil is connected with the outer coil. The coil is preheated to about 70°C, and then directly heated by steam, but the heating process takes a long time, and it takes more than 10 tons of enzymatic hydrolysis liquid to be heated to the end of centrifugation. In this way, this long-term maintenance heating and demulsification method will intensify the oxidation of the microbial oil that has been freed from the cell wall, resulting in poor quality and large loss of oil; the acid added in the acid liquid demulsification It is usually citric acid or phosphoric acid, which will increase the production cost, maintain a certain demulsification time, lead to an increase in peroxide value, and reduce the quality of oil
[0005] In addition, the existing separation method also has a high amount of residual oil in the water phase and residue phase after separation, resulting in low extraction efficiency

Method used

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  • Device and method for online heating separation of microbial oil, and microbial oil
  • Device and method for online heating separation of microbial oil, and microbial oil
  • Device and method for online heating separation of microbial oil, and microbial oil

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preparation example Construction

[0062] Therefore, preferably, the lysate is an enzymatic hydrolyzate of the fermentation liquid of oleaginous microorganisms, and the preparation method of the enzymatic hydrolyzate comprises: contacting the fermented liquid of oleaginous microorganisms with a cell wall lyase in a sterile environment, The enzymolysis solution is obtained by enzymatically hydrolyzing the oleaginous microorganisms in the fermentation broth.

[0063] What needs to be explained in the present invention here is that the sterile environment means that the enzymatic hydrolysis system is in a sterile environment except for the oil-producing microorganisms contained in the fermentation broth.

[0064] According to the present invention, the fermented liquid of the oleaginous microorganism is the fermented liquid obtained directly after the fermentation of the oleaginous microorganism, and does not need any further treatment. The method also saves the concentration of the fermentation broth, the inactiv...

Embodiment approach

[0068] Therefore, according to a preferred embodiment of the present invention, the cell wall lyase includes alkaline protease. In addition, preferably, the cell wall lyase may also include at least one of cellulase, hemicellulase, pectinase, helicase, chitinase and ligninase.

[0069]According to the present invention, the dosage of the cell wall lyase can be selected within a wide range, as long as the cell wall of the oleaginous microorganism can be fully lysed, so as to release the microbial oil. Preferably, the amount of the cell wall lyase is 1-5 g per liter of the fermentation broth.

[0070] According to the present invention, the conditions of the enzymatic hydrolysis can be the conditions conventionally used to crack the cell wall of oleaginous microorganisms by enzymatic hydrolysis, but the inventors of the present invention have found that by performing the enzymatic hydrolysis under aerobic conditions, it is possible to The enzymatic hydrolysis efficiency is furt...

Embodiment 1

[0104] This embodiment is used to illustrate microbial grease provided by the present invention and preparation method thereof

[0105](1) lye preparation: add 200L of demineralized water in the alkali tank, take food-grade NaOH according to the concentration of 20% by weight and put it into the alkali tank to fully dissolve it, and place the prepared lye in a pressure of 0.14MPa. Sterilize in saturated steam at 145°C for 1 hour, then pass sterile compressed air into the alkali tank to make the tank pressure of the alkali tank 0.02-0.05MPa, and pass cooling water into the jacket of the alkali tank to cool the alkali solution to 35-45℃, standby;

[0106] (2) Preparation of cell wall lysate: sterilize the series-connected secondary liquid sterile filtration system with a pressure of 0.14 MPa and a temperature of 145° C. with saturated steam for 1 h, and then use sterile compressed air to keep the pressure for standby; the sterile filtration The filter specification of the syste...

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Abstract

The invention relates to a device and method for online heating separation of microbial oil, and the microbial oil, belonging to the field of extraction of the microbial oil. The device comprises a raw material tank, a heat exchanger, a steam generator, a three-phase centrifuge and a water supply unit, wherein the heat exchanger is separately connected with the raw material tank, the steam generator, the water supply unit and the three-phase centrifuge. The method comprises the following steps: exchanging heat between water and steam to allow produced water to reach a first predetermined temperature, and then allowing the produced water to enter the three-phase centrifuge and to reach a second predetermined temperature; exchanging heat between a lysate and the steam to enable outlet liquidto reach a third predetermined temperature; and allowing the outlet liquid to enter the three-phase centrifuge for separation so as to obtain the microbial oil, wherein the lysate is a lysate of thefermentation broth of oil-producing microorganisms. The device and method of the invention improve the safety of the microbial oil, increase the yield of the microbial oil to 98-99%, allows the quality indexes such as a peroxide value in crude oil to be remarkably improved, and lowers the pollution degree of pollutants such as trichloropropanol.

Description

technical field [0001] The invention relates to the field of separation of microbial oils, in particular to an on-line heating and separation device for microbial oils, a method for on-line separation of microbial oils, and microbial oils extracted by the method. Background technique [0002] Microbial oils are biosynthesized and transformed by microorganisms such as yeast, mold, bacteria, and algae under certain conditions using carbohydrates, hydrocarbons, and ordinary oils as nitrogen sources, carbon sources, and trace elements. [0003] At present, microbial oil extraction mainly has two extraction processes: wet method and dry method. Because the dry extraction process has problems such as high temperature dehydration resulting in large oil oxidation loss, the need to use organic solvent extraction, and high energy consumption, more and more microbial oil extraction will use the wet extraction process. In the wet process, the primary process task is to break the cell w...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/00C11B3/16
CPCC11B1/00C11B1/025C11B3/16
Inventor 瞿瀚鹏曹晟王身健
Owner 梁云
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