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Sartorya Vuill W-10 with high cellulase production, and fermentation method and application thereof

A cellulase and Aspergillus technology, which is applied in the field of fermentation production of cellulase, can solve problems such as affecting the economic benefits of enterprises and increasing production costs, and achieve the effects of being beneficial to commercial application and implementation, increasing production, and improving economic benefits of enterprises

Active Publication Date: 2019-01-11
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing fungi that produce cellulase are mostly compound bacterial agents, and sometimes cellulase needs to be added in the production process to improve the enzymatic hydrolysis rate of lignocellulose, thereby increasing the final ethanol yield. Increase production costs and affect the economic benefits of enterprises

Method used

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  • Sartorya Vuill W-10 with high cellulase production, and fermentation method and application thereof
  • Sartorya Vuill W-10 with high cellulase production, and fermentation method and application thereof
  • Sartorya Vuill W-10 with high cellulase production, and fermentation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The specific preparation process of cellulase prepared by Aspergillus mycelium (Sartorya sp.Vuill W-10) is as follows:

[0045] (1) Preparation of culture medium: Preparing according to potassium nitrate 4g, lactose 4g, urea 0.2g, dipotassium hydrogen phosphate 1.5g, Mandel's nutrient solution 3ml, bran 6g, magnesium sulfate 0.5g, calcium chloride 0.3g, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 3 with 1mol / L hydrochloric acid; then, according to the bottled volume of 43mL / 250mL, the prepared The culture medium is divided into the fermentation vessel;

[0046] (2) Inoculation of Aspergillus miliflora seed liquid: according to 10% of the volume of the culture medium, inoculate the Aspergillus miliflora seed liquid into a fermentation container filled with culture medium;

[0047] (3) Preparation of cellulase: fermen...

Embodiment 2

[0050] The specific preparation process of cellulase prepared by Aspergillus mycelium (Sartorya sp.Vuill W-10) is as follows:

[0051] (1) Preparing the culture medium: 4g of potassium nitrate, 4g of lactose, 0.2g of urea, 1.5g of dipotassium hydrogen phosphate, 6ml of Mandel's nutrient solution, 12g of bran, 0.5g of magnesium sulfate, 0.3g of calcium chloride, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 2.5 with 1mol / L hydrochloric acid; then, according to the bottling volume of 60mL / 250mL, the The culture medium is divided into the fermentation vessel;

[0052] (2) Inoculation of Aspergillus miliformis seed liquid: according to 8% of the medium volume, inoculate the Aspergillus militicum seed liquid in a fermentation vessel filled with medium;

[0053] (3) Preparation of cellulase: ferment the culture inoculated with Asp...

Embodiment 3

[0056] The specific preparation process of cellulase prepared by Aspergillus mycelium (Sartorya sp.Vuill W-10) is as follows:

[0057] (1) Preparation of culture medium: Preparing according to potassium nitrate 4g, lactose 4g, urea 0.2g, dipotassium hydrogen phosphate 1.5g, Mandel's nutrient solution 2ml, bran 4g, magnesium sulfate 0.5g, calcium chloride 0.3g, sodium hydroxide The treated straw powder was prepared at a ratio of 30g to obtain a medium, and the volume was adjusted to 1000mL with deionized water, and the pH value of the medium was adjusted to 3.5 with 3mol / L hydrochloric acid; then, according to the bottled volume of 40mL / 250mL, the prepared The culture medium is divided into the fermentation vessel;

[0058] (2) Inoculation of Aspergillus miliformis seed liquid: according to 15% of the volume of the culture medium, inoculate the Aspergillus miliformis seed liquid in a fermentation container filled with medium;

[0059] (3) Preparation of cellulase: Ferment the ...

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Abstract

The invention discloses a Sartorya Vuill W-10 with high cellulase production, with a preservation No. CGMCC No. 11991. The invention can obtain high-yield cellulose crude enzyme liquor by fermentationof Sartorya Vuill W 10, wherein the filter paper enzyme activity of the cellulase can reach 1.26 U / ml; The cellulase from fermentation can be used in the production of bioethanol, which can effectively improve the enzymatic hydrolysis rate of straw residue and ethanol yield, and has a certain application value. The invention not only has low production cost and high ethanol yield, which is favorable for commercial application and implementation of bioethanol, but also provides a way for comprehensive utilization of waste organisms.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and relates to an aspergillus amylospora capable of realizing high yield of cellulase and a method for fermenting and producing cellulase by using the aspergillus amyrrhizae. Background technique [0002] The resource utilization of lignocellulosic waste such as straw can not only reduce its negative impact on the environment, but also become an effective source of renewable and sustainable energy. For second generation bioethanol production, lignocellulose has become the most viable technology option (Belal, E.B. Bioethanol production from rice straw residues. Braz JMicrobiol. 2013, 44(1):225-234). During the ethanolization process of lignocellulose, cellulase is used to hydrolyze lignocellulose, and the hydrolyzate is fermented to produce bioethanol; among them, the production of cellulase directly affects the hydrolysis of lignocellulose and the subsequent fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12P7/10C12P19/14C12P19/02C12R1/645C12R1/865
CPCC12N9/2437C12P7/10C12P19/02C12P19/14C12N1/145C12R2001/645Y02E50/10
Inventor 刘洋邱忠平孟涛龚正君王冬梅樊超李明星汤国雄
Owner SOUTHWEST JIAOTONG UNIV
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