Fluorescence detection method for risky material fluorine in tea leaves

A fluorescence detection and tea technology, which is applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, etc., can solve the problems of the influence of equipment and reagent conditions, poor detection sensitivity, long detection time, etc., and achieve high sensitivity , low cost, and wide linear detection range

Inactive Publication Date: 2019-01-11
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical defects existing in the prior art, such as: poor detection sensitivity, easily affected by equipment and reagent conditions, high detection cost, long detection time, etc. The present invention provides a Fluorescence detection method of fluorine, through nano-controllable self-assembly to prepare fluorescence signal enhancement substrate, construct a steady-state specific fluoride ion detection system, eliminate background fluorescence, other anion interference and the influence of tea soup color, and realize low-cost and high-efficiency fluorine in tea. Sensitive and specific detection

Method used

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  • Fluorescence detection method for risky material fluorine in tea leaves
  • Fluorescence detection method for risky material fluorine in tea leaves
  • Fluorescence detection method for risky material fluorine in tea leaves

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Embodiment 1

[0035] The invention discloses a fluorescence detection method for the risk substance fluorine in tea. The specific steps are:

[0036] (1) Controllable synthesis of hexagonal upconversion nanocrystals:

[0037] Accurately weigh 0.2367g of yttrium chloride, 0.0775g of ytterbium chloride and 0.0066g of holmium chloride, ultrasonically dissolve them in 12mL of methanol and transfer them to a 250mL three-necked round bottom flask, add 18mL of oleic acid and 42mL of 1-octadecene, Heating and stirring for the first time at 160°C under the protection of argon for 30min, cooling naturally to 50°C, adding 10mL of a methanol mixture with a concentration of 1.482g / mL ammonium fluoride and 1g / mL sodium hydroxide dropwise, stirring and stirring for the second time Heating to 70°C for the first time and maintaining for 50min, heating to 310°C for the third time and maintaining for 1h under the protection of argon, cooling to room temperature, adding ethanol and centrifuging the obtained pr...

Embodiment 2

[0055] For 12 tea standard samples, the method and steps described in Example 1 of the present invention were used to measure the fluorine content of the risk substance in the tea. 5%, can detect the fluorine content in tea with high precision.

[0056] Table 1 detection method of the present invention detects coincidence rate and coefficient of variation of tea standard sample

[0057]

[0058]

[0059] Use the constructed detection method for the detection of other anion standard solutions, ions such as H 2 PO 4 - , Fe 2+ , AcO - , HSO 4 - , NO 3 - , ClO 4 - ,OH - , CN - , I - , Br - , Cl - The addition of ten times the amount of fluoride will not cause the change of the fluorescence signal. Only when the fluoride ion is detected, the color of the system will obviously change to purple, and the up-conversion fluorescence will be significantly weakened, indicating that the constructed method has higher specificity; in addition, in the presence of the abo...

Embodiment 3

[0062] The specific detection method of the present embodiment just changes the time of continuing the reaction in step (2) into 4h and then stops, and other steps and methods are the same as in Example 1.

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Abstract

The invention specifically relates to a fluorescence detection method for risky material fluorine in tea leaves and belongs to the technical field of food safety detection. The fluorescence detectionmethod for risky material fluorine in tea leaves provided by the invention specifically comprises the following steps: taking a methyl alcohol mixed liquor of yttrium chloride, ytterbium chloride, holmium chloride, oleic acid, 1-octadecene, ammonium fluoride and sodium hydroxide as a raw material for preparing a hexagonal up-conversion nano-crystal; adding cyclohexane, IGEPAL CO-520, tetraethyl orthosilicate and APTES, thereby acquiring an aminated hexagonal up-conversion nano-crystal; mixing with a curcumin aqueous solution, thereby acquiring a specific mixed detection system; establishing afluorine ion detection standard curve; determining the fluorine content in the to-be-detected tea leaves. According to the invention, high sensitivity and specificity detection for fluorine in tea leaves is realized in the manner of preparing a fluorescence signal enhanced substrate through nanometer controllable self-assembling and establishing a stable specific fluorine ion detection system, sothat the fluorescence detection method has wider linear detection scope and lower detection limit and is expected to be applied to large-scale detection for risky material fluorine in tea leaves.

Description

technical field [0001] The invention specifically relates to a fluorescence detection method for fluorine, a risk substance in tea, and belongs to the technical field of food safety detection. Background technique [0002] China is the hometown of tea and the birthplace of tea culture. Tea is a pure natural, hygienic, safe, healthy and pollution-free drink that conforms to the physiological health of the human body. Tea tree is a kind of plant that is rich in fluorine element. The leaves are an important place for fluorine element enrichment. The fluorine content per kilogram of tea leaves varies from tens to thousands of milligrams depending on the variety of tea tree and the degree of tenderness of leaves. Appropriate amount of fluoride helps to prevent dental caries, but long-term intake of excessive fluoride will poison the human body, and may even lead to cancer, neurological diseases and dysfunction of the endocrine system. Long-term consumption of brick tea and edge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 郭志明陈全胜张正竹邹小波赵杰文尹丽梅欧阳琴李欢欢
Owner JIANGSU UNIV
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