Preservative agent for preserving Lactobacillus acidophilus and freeze-drying preservation method thereof

Abstract

The invention relates to a preservative agent for preserving Lactobacillus acidophilus and a freeze-drying preservation method thereof. The method mainly achieves the purpose by adjusting the formulaof the protective agent and optimizing the freeze-drying process, and the preservation method comprises: increasing the Lactobacillus acidophilus in the culture dish add the protective agent to the culture dish, and spread the coating solution to obtain the mixed bacterial solution. The mixed bacterial liquid is dispensed into 10 mL penicillin bottle, and each bottle is packed with 0.8-1 mL, and the lid is allowed to stand for 30 minutes. Pre-freeze, freeze and vacuum dry. The protective agent comprises the following components: trehalose 4 to 5%, skim milk powder 10 to 12%, sodium glutamate 0.4 to 0.5%, potassium tripolyphosphate 2 to 3%, gelatin 0.1-0.15%, ascorbic acid 3 to 4%, borate buffer (BBS, pH 8.4) 1 to 3%, the lyophilized Lactobacillus acidophilus of the present invention has high purity, high viable count and long storage period .

Description

Technical field [0001] The invention relates to the technical field of bacterial species preservation, in particular to a preservation and protection agent for Lactobacillus acidophilus and a freeze-drying preservation method. Background technique [0002] Lactobacillus acidophilus belongs to the genus Lactobacillus, a gram-positive bacillus with a slender rod shape. It is a kind of anaerobic or facultative anaerobic microorganisms. It has strong acid resistance and can grow and reproduce in environments where other lactic acid bacteria cannot grow. . A variety of sugars can be used for fermentation. Lactobacillus acidophilus is widely present in the intestines of humans and some animals. It belongs to the beneficial microbial flora. When there is a certain amount, it can beneficially regulate the balance between the beneficial and harmful microbial flora in the intestinal tract, and is useful for maintaining the gastrointestinal tract. Physiological function plays an important...

AI Technical Summary

Problems solved by technology

[0003] With the development of the aquaculture industry in recent years, the problem of antibiotics has continued to emerge, and people have begun to pay attention to microecological preparations. Lactobacillus acidophilus is a strain that is widely used. In the production process of Lactobacillus acidophilus, It is necessary to freeze-dry the Lactobacillus acidophilus strains, that is, to sublimate the microbial cells to be preserved under vacuum conditions and in a frozen state, and finally achieve drying. However, after freeze-drying in an unprotected state, the bacteria will be damaged Mechanical damage, cell membrane permeability changes, dynamic balance is destroyed, and bacteria will die. At present, most of the freeze-drying protective agents for Lactobacillus acidophilus on the market are single substances, which cannot fully protect Lactobacillus acidophilus. The effect is mediocre, and most of the current freeze-drying processes are relatively backward. Therefore, it is particularly important to screen compound protective agents and freeze-drying processes that can maximize the survival rate of bacteria and increase the shelf life.

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