Method for preparing sugar ester through immobilized enzyme catalysis under supercritical CO2 process
An immobilized enzyme, catalytic preparation technology, applied in biochemical equipment and methods, immobilized on/in organic carriers, enzymes, etc., can solve the problems of a large number of organic solvents, long reaction time, complex process, etc. The effect of less environmental pollution, high utilization rate of raw materials, and non-toxic reaction conditions
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Embodiment 1
[0026] (1) Ethylation modification of lipase:
[0027] Dissolve 10 mmol of bromopropionic acid in 10 mL of acetonitrile and add it to a dry round-bottomed flask. After the dissolution is complete, slowly add 1.1 mmol of vinylimidazole dropwise, and reflux at 60 °C for 5 hours with magnetic stirring. After the reaction, use Extract twice with anhydrous ether (5*10mL), remove the yellow oily substance in the lower layer, and vacuum-dry at 70°C for 24 hours to obtain a yellow thick liquid 1-vinyl 3-propionyl imidazolium bromide (modifier);
[0028] Take 1 mmol of 1-vinyl 3-propionyl imidazolium bromide, add 1 mmol carbonyldiimidazole, stir magnetically under the action of 2 mL of anhydrous dimethyl sulfoxide, react at room temperature for 2 h and stop, after the reaction is completed , to obtain the active body; add 90 μL of the active body to 5 mL of Candida rugosa lipase solution with a concentration of 150 μM, stop the reaction after 8 h of magnetic stirring at 0 °C, dialyze f...
Embodiment 2
[0034] (1) Ethylation modification of lipase:
[0035] Dissolve 10 mmol of bromopropionic acid in 10 mL of acetonitrile and add it into a dry round-bottomed flask. After the dissolution is complete, slowly add 1.1 mmol of propenylimidazole dropwise, and reflux at 80°C for 7 hours with magnetic stirring. After the reaction is complete, use Extract twice with anhydrous ether (5*10mL), remove the yellow oily substance in the lower layer, and vacuum-dry at 70°C for 24 hours to obtain a yellow thick liquid 1-propenyl 3-propionyl imidazolium bromide (modifier);
[0036] Take 1 mmol of 1-propenyl 3-propionyl imidazolium bromide, add 1 mmol of carbonyldiimidazole, stir magnetically under the action of 2 mL of anhydrous dimethyl sulfoxide, react at room temperature for 5 h and stop, after the reaction is completed , to obtain the active body; add 90 μL of the active body to 5 mL of Candida antarctica lipase solution with a concentration of 200 μM, stop the reaction after magnetic stirr...
Embodiment 3
[0042] (1) Ethylation modification of lipase:
[0043] Dissolve 10 mmol of bromopropionic acid in 10 mL of acetonitrile and add it into a dry round-bottomed flask. After the dissolution is complete, slowly add 1.1 mmol of vinylimidazole dropwise, and reflux at 100 °C for 10 h with magnetic stirring. After the reaction is complete, use Extract twice with anhydrous ether (5*10mL), remove the yellow oily substance in the lower layer, and vacuum-dry at 70°C for 24 hours to obtain a yellow thick liquid 1-vinyl 3-propionyl imidazolium bromide (modifier);
[0044] Take 1 mmol of 1-vinyl 3-propionyl imidazolium bromide, add 1 mmol carbonyldiimidazole, stir magnetically under the action of 2 mL of anhydrous dimethyl sulfoxide, react at room temperature for 8 h and stop, after the reaction is completed , to obtain the active body; add 90 μL of the active body to 5 mL of porcine pancreatic lipase solution with a concentration of 350 μM, stop the reaction after 24 hours of magnetic stirri...
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